LOCUS       sequence-32184-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Wang Q, Mora-Jensen H, Weniger MA, Perez-Galan P, Wolford C, Hai T, 
            Ron D, Chen W, Trenkle W, Wiestner A, Ye Y
  TITLE     ERAD inhibitors integrate ER stress with an epigenetic mechanism to 
            activate BH3-only protein NOXA in cancer cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Feb 17. 106(7):2200-5.
  PUBMED    19164757
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Feb 17. 106(7):2200-5."
COMMENT     Sequence Label: pRK-ATF4 1-275
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(46..64)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(47..64)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(84..101)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    90..314
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      360..788
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(447..466)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     657..678
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(797..817)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtaagggttg agcggccgct cgagcatgca tctagagggc cctattctat agtgtcacct
       61 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
      121 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
      181 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
      241 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
      301 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
      361 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      421 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
      481 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
      541 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
      601 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
      661 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
      721 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
      781 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
      841 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
      901 ccaaggctcc ccagcaggca gaagtatgca aagcatgcat ctc
//
