LOCUS       sequence-32185-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Wang Q, Mora-Jensen H, Weniger MA, Perez-Galan P, Wolford C, Hai T, 
            Ron D, Chen W, Trenkle W, Wiestner A, Ye Y
  TITLE     ERAD inhibitors integrate ER stress with an epigenetic mechanism to 
            activate BH3-only protein NOXA in cancer cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Feb 17. 106(7):2200-5.
  PUBMED    19164757
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Feb 17. 106(7):2200-5."
COMMENT     Sequence Label: pRK-ATF4 1-275
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(574..597)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      597..606
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(646..665)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(678..696)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(679..696)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tatccacttc actggcccag ctctaaacta aaggaatgat ctggagtgga ggacaggcac
       61 ccctggggaa aggggaagag gttgtaagaa ggtgaagggg gcaacctggt cgggttttgt
      121 taaactttct gggagatggc caattgggtt caccgtctgg gggggctgct tattagtctc
      181 ctggactagg ggggcaaaga gatcacaagt gtcatccaac gtggtcagaa ggtcatctgg
      241 catggtttcc aggtcatcta tacccaacag ggcatccaag tcgaactcct tcaaatccat
      301 tttctccaac atccaatctg tcccggagaa ggcatcctcc ttgctgttgt tggagggact
      361 gaccaaccca tccacagcca gccattcgga ggagcccgcc ttagccttgt cgctggagaa
      421 cccatgaggt ttgaagtgct tggccacctc caggtaatca tctaagagac ctaggctttc
      481 ttcagccccc aaacccgact ggtcgaaggg ggacatcaag tcccccacca acacctcgct
      541 gctcaggaag ctcatttcgg tcatggtcga ccccttgtca tcgtcgtcct tgtagtccat
      601 ggcggcaagc ttagaaccga ggtgcagttg gacctgggag tggacacctg tggagagaaa
      661 ggcaaagtgg atgttattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
      721 atgtattaat tgtagccgcg ttctaacgaa gccaaggggg tgggcctata gactctatag
      781 gcggtactta cgtcactctt ggcacgggga atccgcgttc caatgcaccg ttcccggccg
      841 cgga
//
