LOCUS       sequence-321873-       12141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Eukaryotic expression of human SCN2A variant 1 isoform. This channel
            has been modified to be stably maintained in standard bacterial 
            strains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   DeKeyser JM, Thompson CH, George AL Jr
  TITLE     Cryptic prokaryotic promoters explain instability of recombinant 
            neuronal sodium channels in bacteria.
  JOURNAL   J Biol Chem. 2021 Jan-Jun;296:100298. doi: 
            10.1016/j.jbc.2021.100298. Epub 2021 Jan 15.
  PUBMED    33460646
REFERENCE   2  (bases 1 to 12141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2021 Jan-Jun;296:100298. doi: 10.1016/j.jbc.2021.100298. Epub 
            2021 Jan 15."
COMMENT     Sequence Label: pIR-CMV-SCN2A-Variant-1-IRES-mScarlet
FEATURES             Location/Qualifiers
     source          1..12141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..35
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        374..677
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        678..881
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     832..852
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        927..945
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      977..986
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          5534..5666
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    join(7141..7714,7715..7717,7718..7726)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7308..7325)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7535..7554
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7727..8422
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(7860..7878)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8128..8147
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    8697..8818
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8734..8753)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8788..8807
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8825..9280)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8962..8983)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9174..9193
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9307..9411
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9413..9604
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9436..9456)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             9751..10545
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9751..10545
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9805..9824)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10415..10434
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10733..10752)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10777..10824
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     repeat_region   11054..11116
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      11424..12012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11913..11932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt
       61 tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc
      121 ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt
      181 gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa
      241 ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt
      301 atagataatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
      361 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      421 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      481 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      541 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      601 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      661 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      721 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      781 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      841 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
      901 tccgctagcg attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctggag
      961 ctccaccgcg gtggcggccg ccatggcaca gtcagtgctg gtaccgccag gacctgacag
     1021 cttccgcttc tttaccaggg aatcccttgc tgctattgaa caacgcattg cagaagagaa
     1081 agctaagaga cccaaacagg aacgcaagga tgaggatgat gaaaatggcc caaagccaaa
     1141 cagtgacttg gaagcaggaa aatctcttcc atttatttat ggagacattc ctccagagat
     1201 ggtgtcagtg cccctggagg atctggaccc ctactatatc aataagaaaa cgtttatagt
     1261 attgaataaa gggaaagcaa tctctcgatt cagtgccacc cctgcccttt acattttaac
     1321 tcccttcaac cctattagaa aattagctat taagattttg gtacattctt tattcaatat
     1381 gctcattatg tgcacgattc ttaccaactg tgtatttatg accatgagta accctccaga
     1441 ctggacaaag aatgtggagt atacctttac aggaatttat acttttgaat cacttattaa
     1501 aatacttgca aggggctttt gtttagaaga tttcacattt ttacgggatc catggaattg
     1561 gttggatttc acagtcatta cttttgcata tgtgacagag tttgtggacc tgggcaatgt
     1621 ctcagcgttg agaacattca gagttctccg agcattgaaa acaatttcag tcattccagg
     1681 cctgaagacc attgtggggg ccctgatcca gtcagtgaag aagctttctg atgtcatgat
     1741 cttgactgtg ttctgtctaa gcgtgtttgc gctaatagga ttgcagttgt tcatgggcaa
     1801 cctacgaaat aaatgtttgc aatggcctcc agataattct tcctttgaaa taaatatcac
     1861 ttccttcttt aacaattcat tggatgggaa tggtactact ttcaatagga cagtgagcat
     1921 atttaactgg gatgaatata ttgaggataa aagtcacttt tattttttag aggggcaaaa
     1981 tgatgctctg ctttgtggca acagctcaga tgcaggccag tgtcctgaag gatacatctg
     2041 tgtgaaggct ggtagaaacc ccaactatgg ctacacgagc tttgacacct ttagttgggc
     2101 ctttttgtcc ttatttcgtc tcatgactca agacttctgg gaaaaccttt atcaactgac
     2161 actacgtgct gctgggaaaa cgtacatgat attttttgtg ctggtcattt tcttgggctc
     2221 attctatcta ataaatttga tcttggctgt ggtggccatg gcctatgagg aacagaatca
     2281 ggccacattg gaagaggctg aacagaagga agctgaattt cagcagatgc tcgaacagtt
     2341 gaaaaagcaa caagaagaag ctcaggcggc agctgcagcc gcatctgctg aatcaagaga
     2401 cttcagtggt gctggtggga taggagtttt ttcagagagt tcttcagtag catctaagtt
     2461 gagctccaaa agtgaaaaag agctgaaaaa cagaagaaag aaaaagaaac agaaagaaca
     2521 gtctggagaa gaagagaaaa atgacagagt ccgaaaatcg gaatctgaag acagcataag
     2581 aagaaaaggt ttccgttttt ccttggaagg aagtaggctg acatatgaaa agagattttc
     2641 ttctccacac cagtccttac tgagcatccg tggctccctt ttctctccaa gacgcaacag
     2701 tagggcgagc cttttcagct tcagaggtcg agcaaaggac attggctctg agaatgactt
     2761 tgctgatgat gagcacagca cctttgagga caatgacagc cgaagagact ctctgttcgt
     2821 gccgcacaga catggagaac ggcgccacag caatgtcagc caggccagcc gtgcctccag
     2881 ggtgctcccc atcctgccca tgaatgggaa gatgcatagc gctgtggact gcaatggtgt
     2941 ggtctccctg gtcgggggcc cttctaccct cacatctgct gggcagctcc taccagaggg
     3001 cacaactact gaaacagaaa taagaaagag acggtccagt tcttatcatg tttccatgga
     3061 tttattggaa gatcctacat caaggcaaag agcaatgagt atagccagta ttttgaccaa
     3121 caccatggaa gaacttgaag aatccagaca gaaatgccca ccatgctggt ataaatttgc
     3181 taatatgtgt ttgatttggg actgttgtaa accatggtta aaggtgaaac accttgtcaa
     3241 cctggttgta atggacccat ttgttgacct ggccatcacc atctgcattg tcttaaatac
     3301 actcttcatg gctatggagc actatcccat gacggagcag ttcagcagtg tactgtctgt
     3361 tggaaacctg gtcttcacag ggatcttcac agcagaaatg tttctcaaga taattgccat
     3421 ggatccatat tattactttc aagaaggctg gaatattttt gatggtttta ttgtgagcct
     3481 tagtttaatg gaacttggtt tggcaaatgt ggaaggattg tcagttctcc gatcattccg
     3541 gctgctccga gttttcaagt tggcaaaatc ttggccaact ctaaatatgc taattaagat
     3601 cattggcaat tctgtggggg ctctaggaaa cctcaccttg gtattggcca tcatcgtctt
     3661 catttttgct gtggtcggca tgcagctctt tggtaagagc tacaaagaat gtgtctgcaa
     3721 gatttccaat gattgtgaac tcccacgctg gcacatgcat gactttttcc actccttcct
     3781 gatcgtgttc cgcgtgctgt gtggagagtg gatagagacc atgtgggact gtatggaggt
     3841 cgctggccaa accatgtgcc ttactgtctt catgatggtc atggtgattg gaaatctagt
     3901 ggttctgaac ctcttcttgg ccttgctttt gagttccttc agttctgaca atcttgctgc
     3961 cactgatgat gataacgaaa tgaataatct ccagattgct gtgggaagga tgcagaaagg
     4021 aatcgatttt gttaaaagaa aaatacgtga atttattcag aaagcctttg ttaggaagca
     4081 gaaagcttta gatgaaatta aaccgcttga agatctaaat aataaaaaag acagctgtat
     4141 ttccaaccat accaccatag aaataggcaa agacctcaat tatctcaaag acggaaatgg
     4201 aactactagt ggcataggca gcagtgtaga aaaatatgtc gtggatgaaa gtgattacat
     4261 gtcatttata aacaacccta gcctcactgt gacagtacca attgctgttg gagaatctga
     4321 ctttgaaaat ttaaatactg aagaattcag cagcgagtca gatatggagg aaagcaaaga
     4381 gaagctaaat gcaactagtt catctgaagg cagcacggtt gatattggag ctcccgccga
     4441 gggagaacag cctgaggttg aacctgagga atcccttgaa cctgaagcct gttttacaga
     4501 agactgtgta cggaagttca agtgttgtca gataagcata gaagaaggca aagggaaact
     4561 ctggtggaat ttgaggaaaa catgctataa gatagtggag cacaattggt tcgaaacctt
     4621 cattgtcttc atgattctgc tgagcagtgg ggctctggcc tttgaagata tatacattga
     4681 gcagcgaaaa accattaaga ccatgttaga atatgctgac aaggttttca cttacatatt
     4741 cattctggaa atgctgctaa agtgggttgc atatggtttt caagtgtatt ttaccaatgc
     4801 ctggtgctgg ctagacttcc tgattgttga tgtctcactg gttagcttaa ctgcaaatgc
     4861 cttgggttac tcagaacttg gtgccatcaa atccctcaga acactaagag ctctgaggcc
     4921 actgagagct ttgtcccggt ttgaaggaat gagggttgtt gtaaatgctc ttttaggagc
     4981 cattccatct atcatgaatg tacttctggt ttgtctgatc ttttggctaa tattcagtat
     5041 catgggagtg aatctctttg ctggcaagtt ttaccattgt attaattaca ccactggaga
     5101 gatgtttgat gtaagcgtgg tcaacaacta cagtgagtgc aaagctctca ttgagagcaa
     5161 tcaaactgcc aggtggaaaa atgtgaaagt aaactttgat aacgtaggac ttggatatct
     5221 gtctctactt caagtagcca cgtttaaggg atggatggat attatgtatg cagctgttga
     5281 ttcacgaaat gtagaattac aacccaagta tgaagacaac ctgtacatgt atctttattt
     5341 tgtcatcttt attatttttg gttcattctt taccttgaat cttttcattg gtgtcatcat
     5401 agataacttc aaccaacaga aaaagaagtt tggaggtcaa gacattttta tgacagaaga
     5461 acagaagaaa tactacaatg caatgaaaaa actgggttca aagaaaccac aaaaacccat
     5521 acctcgacct gctgtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact
     5581 gggcttgtcg agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga
     5641 catccacttt gcctttctct ccacagaaca aattccaagg aatggtcttt gattttgtaa
     5701 ccaaacaagt ctttgatatc agcatcatga tcctcatctg ccttaacatg gtcaccatga
     5761 tggtggaaac cgatgaccag agtcaagaaa tgacaaacat tctgtactgg attaatctgg
     5821 tgtttattgt tctgttcact ggagaatgtg tgctgaaact gatctctctt cgttactact
     5881 atttcactat tggatggaat atttttgatt ttgtggtggt cattctctcc attgtaggaa
     5941 tgtttctggc tgaactgata gaaaagtatt ttgtgtcccc taccctgttc cgagtgatcc
     6001 gtcttgccag gattggccga atcctacgtc tgatcaaagg agcaaagggg atccgcacgc
     6061 tgctctttgc tttgatgatg tcccttcctg cgttgtttaa catcggcctc cttcttttcc
     6121 tggtcatgtt catctacgcc atctttggga tgtccaattt tgcctatgtt aagagggaag
     6181 ttgggatcga tgacatgttc aactttgaga cctttggcaa cagcatgatc tgcctgttcc
     6241 aaattacaac ctctgctggc tgggatggat tgctagcacc tattcttaat agtggacctc
     6301 cagactgtga ccctgacaaa gatcaccctg gaagctcagt taaaggagac tgtgggaacc
     6361 catctgttgg gattttcttt tttgtcagtt acatcatcat atccttcctg gttgtggtga
     6421 acatgtacat cgcggtcatc ctggagaact tcagtgttgc tactgaagaa agtgcagagc
     6481 ctctgagtga ggatgacttt gagatgttct atgaggtttg ggagaagttt gatcccgatg
     6541 cgacccagtt tatagagttt gccaaacttt ctgattttgc agatgccctg gatcctcctc
     6601 ttctcatagc aaaacccaac aaagtccagc tcattgccat ggatctgccc atggtgagtg
     6661 gtgaccggat ccactgtctt gacatcttat ttgcttttac aaagcgtgtt ttgggtgaga
     6721 gtggagagat ggatgccctt cgaatacaga tggaagagcg attcatggca tcaaacccct
     6781 ccaaagtctc ttatgagccc attacgacca cgttgaaacg caaacaagag gaggtgtctg
     6841 ctattattat ccagagggct tacagacgct acctcttgaa gcaaaaagtt aaaaaggtat
     6901 caagtatata caagaaagac aaaggcaaag aatgtgatgg aacacccatc aaagaagata
     6961 ctctcattga taaactgaat gagaattcaa ctccagagaa aaccgatatg acgccttcca
     7021 ccacgtctcc accctcgtat gatagtgtga ccaaaccaga aaaagaaaaa tttgaaaaag
     7081 acaaatcaga aaaggaagac aaagggaaag atatcaggga aagtaaaaag taaggatccg
     7141 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     7201 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     7261 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     7321 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     7381 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     7441 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     7501 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     7561 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     7621 cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg
     7681 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg tgagcaaggg
     7741 cgaggcagtg atcaaggagt tcatgcggtt caaggtgcac atggagggct ccatgaacgg
     7801 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     7861 caagctgaag gtgaccaagg gtggccccct gcccttctcc tgggacatcc tgtcccctca
     7921 gttcatgtac ggctccaggg ccttcaccaa gcaccccgcc gacatccccg actactataa
     7981 gcagtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc
     8041 cgtgaccgtg acccaggaca cctccctgga ggacggcacc ctgatctaca aggtgaagct
     8101 ccgcggcacc aacttccctc ctgacggccc cgtaatgcag aagaagacaa tgggctggga
     8161 agcgtccacc gagcggttgt accccgagga cggcgtgctg aagggcgaca ttaagatggc
     8221 cctgcgcctg aaggacggag gccgctacct ggcggacttc aagaccacct acaaggccaa
     8281 gaagcccgtg cagatgcccg gcgcctacaa cgtcgaccgc aagttggaca tcacctccca
     8341 caacgaggac tacaccgtgg tggaacagta cgaacgctcc gagggccgcc actccaccgg
     8401 cggcatggac gagctgtaca agtaacctgc aggaattccc cttcccaaca gttgcgcagc
     8461 ctgaatggcg aatggagatc caatttttaa gtgtataatg tgttaaacta ctgattctaa
     8521 ttgtttgtgt attttagatt cacagtccca aggctcattt caggcccctc agtcctcaca
     8581 gtctgttcat gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     8641 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     8701 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8761 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac
     8821 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     8881 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     8941 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     9001 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     9061 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     9121 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     9181 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     9241 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     9301 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9361 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagaa tcctgaggcg
     9421 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     9481 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     9541 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     9601 tcccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     9661 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     9721 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     9781 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     9841 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     9901 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     9961 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
    10021 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
    10081 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
    10141 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
    10201 ggtcttgtcg atcaggatga tctggacgaa gaacatcagg ggctcgcgcc agccgaactg
    10261 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
    10321 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
    10381 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
    10441 gaacttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
    10501 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
    10561 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
    10621 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
    10681 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
    10741 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
    10801 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
    10861 tcgatacccc actgcctaac gcgtttttgt tactttatag aagaaatttt gagtttttgt
    10921 ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt
    10981 aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca
    11041 gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat
    11101 gattatcttt ctagggttaa acgcgttagg catacgcatc caccgagacc ccattggggc
    11161 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
    11221 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
    11281 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    11341 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    11401 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    11461 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    11521 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    11581 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    11641 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    11701 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    11761 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    11821 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    11881 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    11941 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    12001 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    12061 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    12121 atgcattagt tattaattta a
//
