LOCUS       sequence-32188-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Wang Q, Mora-Jensen H, Weniger MA, Perez-Galan P, Wolford C, Hai T, 
            Ron D, Chen W, Trenkle W, Wiestner A, Ye Y
  TITLE     ERAD inhibitors integrate ER stress with an epigenetic mechanism to 
            activate BH3-only protein NOXA in cancer cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Feb 17. 106(7):2200-5.
  PUBMED    19164757
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Feb 17. 106(7):2200-5."
COMMENT     Sequence Label: pRK-ATF4 111-351
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(263..281)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(264..281)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(301..318)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    307..531
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(664..683)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     874..895
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaactggata agaagctgaa aaaaatggag caaaacaaga cagcagccac taggtaccgc
       61 cagaagaaga gggcggagca ggaggctctt actggtgagt gcaaagagct ggaaaagaag
      121 aacgaggctc taaaagagag ggcggattcc ctggccaagg agatccagta cctgaaagat
      181 ttgatagaag aggtccgcaa ggcaaggggg aagaaaaggg tcccctaggc ggccgctcga
      241 gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
      301 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      361 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      421 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
      481 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
      541 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
      601 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      661 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      721 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      781 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      841 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      901 cactcaaccc tatctcggtc tattcttttg a
//
