LOCUS       sequence-32189-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Wang Q, Mora-Jensen H, Weniger MA, Perez-Galan P, Wolford C, Hai T, 
            Ron D, Chen W, Trenkle W, Wiestner A, Ye Y
  TITLE     ERAD inhibitors integrate ER stress with an epigenetic mechanism to 
            activate BH3-only protein NOXA in cancer cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Feb 17. 106(7):2200-5.
  PUBMED    19164757
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Feb 17. 106(7):2200-5."
COMMENT     Sequence Label: pRK-ATF4 111-351
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(254..277)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      277..286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(326..345)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(358..376)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(359..376)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(586..610)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(607..810)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(636..656)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cttcatgata tccaccttca ctggcccagc ctctaaacta aaggaatgat ctggagtgga
       61 ggacaggcac ccctggggaa aggggaagag gttgtaagaa ggtgaagggg gcaacctggt
      121 cgggttttgt taaactttct gggagatggc caattgggtt caccgtctgg gggggctgct
      181 tattagtctc ctggactagg ggggcaaaga gatcacaagt gtcatccaac gtggtcagaa
      241 ggtcggtcga ccccttgtca tcgtcgtcct tgtagtccat ggcggcaagc ttagaaccga
      301 ggtgcagttg gacctgggag tggacacctg tggagagaaa ggcaaagtgg atgttattct
      361 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
      421 ttctaacgaa gccaaggggg tgggcctata gactctatag gcggtactta cgtcactctt
      481 ggcacgggga atccgcgttc caatgcaccg ttcccggccg cggaggctgg atcggtcccg
      541 gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac
      601 taaacgagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      661 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      721 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      781 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      841 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatg
//
