LOCUS       sequence-321901-        9326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human GPT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC6-GPT1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1613..1678
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    1726..1843
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1892..2402
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2321..2339
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2411..2806
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             2878..3111
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3183..3304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3220..3239)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3274..3293
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3344..3479
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3499..3518)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3500..3518)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3528..3545)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3528..3544)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3537..3559)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3686..4141
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3773..3792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3983..4004
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4167..4271
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4272..4340,4341..5132)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4490..4509)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5303..5891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5792..5811
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6045..6062
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6179..6200
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6215..6220,6221..6238,6239..6245)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6253..6269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6258..6280
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6277..6293
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6277..6293
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6312..6332
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6314..6332
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6360..6586
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6587..6767
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6814..6939
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7432..7665
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7850..7894
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        join(8183..9326,1..68)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     9231..9250
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt
       61 tgttagacga agcgctagcg ctaccggttt aattaataga gtcatggcga gcagcacagg
      121 agatcgaagt caggcagttc gacacggttt gcgcgccaaa gttttgacgc tggatggcat
      181 gaatccgagg gtgcgccgcg tggaatatgc agtgcgagga cccattgtgc agagagctct
      241 tgaacttgag caggagctgc gccagggggt gaaaaagccg tttactgagg ttatacgggc
      301 gaacatcgga gacgctcaag cgatgggcca acgacccatt acatttcttc ggcaggtgtt
      361 ggcgctttgc gtaaatccgg atttgttgtc ttcccccaat ttccctgatg atgcgaaaaa
      421 gagggccgag agaatcctcc aagcatgcgg tgggcattca cttggcgcat actctgtctc
      481 cagtggaata caactgattc gcgaagacgt tgcccgctac atagagcgcc gcgacggagg
      541 gatccctgct gatccgaata acgttttctt gagtaccgga gctagcgatg ctattgtaac
      601 tgtacttaaa ttgttggtgg ccggtgaggg acacacgaga acgggagttc tgatcccgat
      661 cccccaatat cctctttata gcgcgacgtt ggctgagctc ggtgcggtgc aagtagacta
      721 ttatttggac gaggaacgag cctgggcctt ggatgtggca gagctccatc gggccttggg
      781 gcaagcccga gatcattgtc gccccagagc cctttgcgtg atcaatccag gtaacccgac
      841 cggtcaggta cagactaggg agtgtattga ggcagtaatc aggtttgctt ttgaggagcg
      901 cctgtttttg cttgcggatg aggtttatca agataatgtg tacgcggctg gatcacagtt
      961 tcacagtttt aaaaaggtac ttatggagat gggtccacct tacgcgggtc aacaagagct
     1021 tgccagtttt cacagcacgt ctaaaggata tatgggtgaa tgtggattca gagggggata
     1081 tgttgaagtt gtaaacatgg atgccgcagt gcaacaacag atgttgaaac tgatgagtgt
     1141 tcggctgtgc ccacccgtac caggtcaagc gcttctggac ttggtcgttt ccccccctgc
     1201 acccacagac cctagcttcg cacaattcca agccgaaaaa caagctgtcc tcgcggaact
     1261 ggcagcgaag gcaaagctca cagaacaggt attcaacgag gccccaggca tcagttgtaa
     1321 tcctgtgcaa ggcgcaatgt actctttccc gagggtccag ctccccccta gagctgtgga
     1381 acgagcgcag gagctcggct tggcacccga catgttcttc tgtttgcggc ttctggagga
     1441 gacgggtatt tgcgttgtac cagggagcgg ctttgggcaa cgagaaggga cgtatcattt
     1501 tcgcatgaca atactcccgc cgctcgaaaa actcagactc cttttggaga agctgtctcg
     1561 gttccatgct aaattcacgt tggaatactc cggaggcagc gcggccgcag gcgactacaa
     1621 agaccatgac ggtgattata aagatcatga cattgattac aaggatgacg atgacaagta
     1681 ataagaattc tcgacctcga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1741 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1801 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1861 cagcagagat ccactttggc cgcggctcga gggggttggg gttgcgcctt ttccaaggca
     1921 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     1981 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     2041 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     2101 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     2161 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     2221 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     2281 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     2341 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     2401 agggggatcc atggttcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     2461 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     2521 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     2581 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     2641 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     2701 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     2761 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaaggta cctttaagac
     2821 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     2881 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     2941 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3001 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3061 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     3121 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     3181 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3241 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3301 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     3361 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3421 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3481 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     3541 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     3601 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3661 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3721 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3781 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3841 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3901 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3961 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4021 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4081 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     4141 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4201 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4261 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4321 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4381 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4441 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4501 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4561 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4621 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4681 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4741 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4801 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4861 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4921 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4981 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5041 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5101 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5161 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5221 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5281 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5341 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5401 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5461 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5521 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5581 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5641 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5701 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5761 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5821 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5881 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5941 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6001 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6061 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6121 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6181 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6241 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     6301 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     6361 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     6421 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     6481 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     6541 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     6601 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6661 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6721 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     6781 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     6841 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     6901 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     6961 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7021 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     7081 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     7141 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     7201 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     7261 accaccgcac agcaagcagc agctgatctt cagacctgga ggaggagata tgagggacaa
     7321 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     7381 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     7441 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     7501 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     7561 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     7621 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     7681 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     7741 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     7801 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     7861 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     7921 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     7981 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     8041 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     8101 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgatcacg
     8161 agactagcct cgagcgggat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc
     8221 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct
     8281 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
     8341 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
     8401 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
     8461 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
     8521 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg
     8581 gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga
     8641 cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct
     8701 gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag
     8761 acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa
     8821 gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc
     8881 tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt
     8941 cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg
     9001 gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg
     9061 gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg
     9121 cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt
     9181 gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg
     9241 gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt
     9301 tgaaatgtaa tcatttgggt caatat
//
