LOCUS       sequence-321902-        8660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human Rap2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8660)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 8660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC5-Rap2A-3xFLAG
FEATURES             Location/Qualifiers
     source          1..8660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     259..278
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1028..1093
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    1141..1258
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1307..1817
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1736..1754
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1839..2438
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1839..1858)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2335..2355
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2566..2799
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2871..2992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2908..2927)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2962..2981
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3032..3167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3187..3206)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3188..3206)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3216..3233)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3216..3232)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3225..3247)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3374..3829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3461..3480)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3671..3692
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3855..3959
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3960..4028,4029..4820)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4178..4197)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4991..5579
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5480..5499
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5733..5750
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5867..5888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5903..5908,5909..5926,5927..5933)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5941..5957
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5946..5968
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5965..5981
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5965..5981
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6000..6020
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6002..6020
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6048..6274
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6275..6455
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6502..6627
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7120..7353
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7538..7582
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        join(7871..8660,1..422)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
       61 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
      121 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
      181 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
      241 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
      301 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
      361 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
      421 acgaagcgct agcgctaccg gtttaattaa agggacgatg cgcgagtaca aagtggtggt
      481 gctgggctcg ggcggggtag gcaaatccgc cctgaccgtg cagttcgtga ccggcacctt
      541 catcgagaaa tacgacccca ccatcgagga cttctaccgc aaggagatcg aggtggattc
      601 gtcgccgtcg gtgctggaga tcctggacac ggcgggcacc gagcagttcg cgtccatgcg
      661 ggacctgtac atcaagaacg gccagggctt catcctcgtc tacagcctcg tcaaccagca
      721 gagcttccag gacatcaagc ccatgcggga ccagatcatc cgcgtgaagc ggtatgagaa
      781 agtgccagtc atcttggttg ggaacaaagt ggacctggaa agtgagagag aagtatcgtc
      841 cagcgaaggc agagcccttg ctgaagagtg gggctgcccc tttatggaaa cttccgctaa
      901 gagtaaaaca atggtggacg aactctttgc agaaattgtg aggcagatga actatgctgc
      961 tcagcctgac aaagatgacc catgctgttc tgcatgtaac atacaaggag gcagcgcggc
     1021 cgcaggcgac tacaaagacc atgacggtga ttataaagat catgacattg attacaagga
     1081 tgacgatgac aagtaataag aattctcgac ctcgagacaa atggcagtat tcatccacaa
     1141 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     1201 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     1261 ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc
     1321 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     1381 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     1441 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     1501 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     1561 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     1621 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     1681 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     1741 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     1801 ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac aagcccacgg
     1861 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     1921 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     1981 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     2041 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     2101 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     2161 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     2221 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     2281 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     2341 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     2401 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
     2461 gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca atgacttaca
     2521 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     2581 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     2641 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     2701 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     2761 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     2821 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     2881 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2941 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3001 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3061 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3121 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     3181 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     3241 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3301 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3361 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3421 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3481 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3541 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3601 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3661 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     3721 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     3781 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     3841 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3901 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3961 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4021 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4081 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4141 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4201 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4261 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4321 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4381 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4441 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4501 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4561 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4621 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4681 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4741 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4801 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4861 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4921 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4981 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5041 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5101 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5161 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5221 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5281 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5341 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5401 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5461 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5521 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5581 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5641 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5701 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5761 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5821 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     5881 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     5941 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     6001 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     6061 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     6121 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     6181 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     6241 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     6301 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6361 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6421 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     6481 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     6541 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     6601 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     6661 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     6721 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     6781 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     6841 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     6901 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     6961 caagcagcag ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     7021 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     7081 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     7141 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     7201 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     7261 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     7321 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     7381 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     7441 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     7501 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     7561 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     7621 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     7681 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     7741 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     7801 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     7861 agcgggatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg
     7921 gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg
     7981 ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag
     8041 gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga
     8101 acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt
     8161 gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg
     8221 ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg
     8281 ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg
     8341 gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt
     8401 ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg
     8461 cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc
     8521 ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
     8581 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
     8641 gggcggcagt tatggcggtg
//
