LOCUS       sequence-321903-        8926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human GPT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 8926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC2-GPT1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..8926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     373..392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     626..643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    760..781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(796..801,802..819,820..826)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    834..850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     839..861
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     858..874
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     858..874
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     893..913
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        895..913
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        941..1167
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1168..1348
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1395..1520
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2013..2246
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2431..2475
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2773..2793
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3082..3285
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3236..3256
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4847..4912
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4960..5077
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5126..5636
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5555..5573
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5658..6257
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5658..5677)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6154..6174
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6385..6618
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6690..6811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6727..6746)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6781..6800
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6851..6986
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7006..7025)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7007..7025)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7035..7052)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7035..7051)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7044..7066)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7193..7648
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7280..7299)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7490..7511
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7674..7778
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7779..7847,7848..8639)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7997..8016)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8810..8926,1..472)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
       61 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      301 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      481 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      541 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      601 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      661 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      721 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      781 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      841 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
      901 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
      961 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     1021 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     1081 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     1141 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     1201 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1261 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1321 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     1381 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     1441 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     1501 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     1561 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     1621 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     1681 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     1741 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     1801 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcag
     1861 cagctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     1921 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     1981 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     2041 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     2101 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     2161 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     2221 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     2281 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     2341 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     2401 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     2461 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     2521 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     2581 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     2641 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     2701 tccattcgat tagtgaacgg atctcgacgg tatcgatcac gagactagcc tcgagcagca
     2761 gcccccttca ccgagggcct atttcccatg attccttcat atttgcatat acgatacaag
     2821 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
     2881 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     2941 atggactatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     3001 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     3061 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     3121 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     3181 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     3241 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     3301 cagatccgct agcgctaccg gtttaattaa tagagtcatg gcgagcagca caggagatcg
     3361 aagtcaggca gttcgacacg gtttgcgcgc caaagttttg acgctggatg gcatgaatcc
     3421 gagggtgcgc cgcgtggaat atgcagtgcg aggacccatt gtgcagagag ctcttgaact
     3481 tgagcaggag ctgcgccagg gggtgaaaaa gccgtttact gaggttatac gggcgaacat
     3541 cggagacgct caagcgatgg gccaacgacc cattacattt cttcggcagg tgttggcgct
     3601 ttgcgtaaat ccggatttgt tgtcttcccc caatttccct gatgatgcga aaaagagggc
     3661 cgagagaatc ctccaagcat gcggtgggca ttcacttggc gcatactctg tctccagtgg
     3721 aatacaactg attcgcgaag acgttgcccg ctacatagag cgccgcgacg gagggatccc
     3781 tgctgatccg aataacgttt tcttgagtac cggagctagc gatgctattg taactgtact
     3841 taaattgttg gtggccggtg agggacacac gagaacggga gttctgatcc cgatccccca
     3901 atatcctctt tatagcgcga cgttggctga gctcggtgcg gtgcaagtag actattattt
     3961 ggacgaggaa cgagcctggg ccttggatgt ggcagagctc catcgggcct tggggcaagc
     4021 ccgagatcat tgtcgcccca gagccctttg cgtgatcaat ccaggtaacc cgaccggtca
     4081 ggtacagact agggagtgta ttgaggcagt aatcaggttt gcttttgagg agcgcctgtt
     4141 tttgcttgcg gatgaggttt atcaagataa tgtgtacgcg gctggatcac agtttcacag
     4201 ttttaaaaag gtacttatgg agatgggtcc accttacgcg ggtcaacaag agcttgccag
     4261 ttttcacagc acgtctaaag gatatatggg tgaatgtgga ttcagagggg gatatgttga
     4321 agttgtaaac atggatgccg cagtgcaaca acagatgttg aaactgatga gtgttcggct
     4381 gtgcccaccc gtaccaggtc aagcgcttct ggacttggtc gtttcccccc ctgcacccac
     4441 agaccctagc ttcgcacaat tccaagccga aaaacaagct gtcctcgcgg aactggcagc
     4501 gaaggcaaag ctcacagaac aggtattcaa cgaggcccca ggcatcagtt gtaatcctgt
     4561 gcaaggcgca atgtactctt tcccgagggt ccagctcccc cctagagctg tggaacgagc
     4621 gcaggagctc ggcttggcac ccgacatgtt cttctgtttg cggcttctgg aggagacggg
     4681 tatttgcgtt gtaccaggga gcggctttgg gcaacgagaa gggacgtatc attttcgcat
     4741 gacaatactc ccgccgctcg aaaaactcag actccttttg gagaagctgt ctcggttcca
     4801 tgctaaattc acgttggaat actccggagg cagcgcggcc gcaggcgact acaaagacca
     4861 tgacggtgat tataaagatc atgacattga ttacaaggat gacgatgaca agtaataaga
     4921 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     4981 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     5041 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     5101 agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg
     5161 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     5221 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     5281 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     5341 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     5401 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     5461 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     5521 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     5581 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
     5641 atccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     5701 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     5761 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     5821 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     5881 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     5941 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     6001 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     6061 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     6121 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     6181 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     6241 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
     6301 gaccccatgc atcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6361 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6421 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6481 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6541 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6601 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6661 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6721 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6781 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6841 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6901 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6961 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     7021 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7081 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7141 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     7201 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7261 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7321 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7381 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7441 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7501 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7561 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7621 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     7681 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7741 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7801 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7861 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7921 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7981 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8041 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8101 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8161 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8221 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8281 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8341 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8401 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8461 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8521 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8581 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8641 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8701 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8761 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8821 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8881 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactgg
//
