LOCUS       sequence-321905-        9407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human GPT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 9407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC6-GPT2-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1824..3035
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2872..2891
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4217..4226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4661..4726
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4774..4891
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4940..5450
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5369..5387
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5459..5854
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5926..6159
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6231..6352
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6268..6287)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6322..6341
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6392..6527
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6547..6566)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6548..6566)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6576..6593)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6576..6592)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6585..6607)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6734..7189
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6821..6840)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7031..7052
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7215..7319
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7320..7388,7389..8180)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7538..7557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8351..8939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8840..8859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9093..9110
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9227..9248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9263..9268,9269..9286,9287..9293)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9301..9317
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9306..9328
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9325..9341
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9325..9341
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9360..9380
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9362..9380
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     1861 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     1921 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     1981 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2041 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2101 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2161 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2221 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2281 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2341 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2401 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2461 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2521 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2581 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2641 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2701 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     2761 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     2821 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     2881 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     2941 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3001 ctaaattctg gccgtttttg gcttttttgt tagacgaagc gctagcgcta ccggtttaat
     3061 taagcgcgcg atgcagcggg cggcagcact ggtcaggcga ggctgtggtc ctcggactcc
     3121 aagctcctgg ggcagaagcc agagtagcgc tgctgccgaa gcctcggcgg tgctcaaggt
     3181 gcggcccgag cgcagccggc gcgagcgcat cctcacgctg gagtccatga acccgcaggt
     3241 gaaggcggtg gagtacgccg tgcggggacc catcgtgctc aaggccggcg agatcgagct
     3301 cgagctgcag cggggtatca aaaagccatt cacagaggtc atccgagcca acatcgggga
     3361 cgcccaggct atggggcagc agccaatcac cttcctccgg caggtgatgg cactatgcac
     3421 ctacccaaac ctgctggaca gccccagctt cccagaagat gctaagaaac gtgcccggcg
     3481 gatcctgcag gcttgtggcg ggaacagcct ggggtcctac agtgctagcc agggtgtcaa
     3541 ctgcatccgt gaagatgtgg ctgcctacat caccaggagg gatggcggtg tgcctgcgga
     3601 ccccgacaac atctacctga ccacgggagc tagtgacggc atttctacga tcctgaagat
     3661 cctcgtctcc gggggcggca agtcacggac aggtgtgatg atccccatcc cacaatatcc
     3721 cctctattca gctgtcatct ctgagctcga cgccatccag gtgaattact acctggacga
     3781 ggagaactgc tgggcgctca acgttaacga actgcgccgg gcggtgcagg aggccaaaga
     3841 ccactgtgat cctaaggtgc tctgcataat caaccctggg aaccccacag gccaggtaca
     3901 aagcagaaag tgcatagaag atgtgatcca ctttgcctgg gaagagaagc tctttctcct
     3961 ggctgatgag gtgtaccagg acaacgtgta ctctccagat tgcagattcc actccttcaa
     4021 gaaggtgctg tacgagatgg ggcccgagta ctccagcaac gtggagctcg cctccttcca
     4081 ctccacctcc aagggctaca tgggcgagtg tggttacaga ggaggctaca tggaggtgat
     4141 caacctgcac cctgagatca agggccagct ggtgaagctg ctgtcggtgc gcctgtgccc
     4201 cccagtgtct gggcaggccg ccatggacat tgtcgtgaac cccccggtgg caggagagga
     4261 gtcctttgag caattcagcc gagagaagga gtcggtcctg ggtaatctgg ccaaaaaagc
     4321 aaagctgacg gaagacctgt ttaaccaagt cccaggaatt cactgcaacc ccttgcaggg
     4381 ggccatgtac gccttccctc ggatcttcat tcctgccaaa gctgtggagg ctgctcaggc
     4441 ccatcaaatg gctccagaca tgttctactg catgaagctc ctggaggaga ctggcatctg
     4501 tgtcgtgccc ggcagtggct ttgggcagag ggaaggcact taccacttca ggatgactat
     4561 cctccctcca gtggagaagc tgaaaacggt gctgcagaag gtgaaagact tccacatcaa
     4621 cttcctggag aagtacgcgg gaggcagcgc ggccgcaggc gactacaaag accatgacgg
     4681 tgattataaa gatcatgaca ttgattacaa ggatgacgat gacaagtaat aagaattctc
     4741 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     4801 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     4861 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     4921 actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg
     4981 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     5041 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     5101 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     5161 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     5221 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     5281 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     5341 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     5401 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccat
     5461 ggttcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
     5521 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
     5581 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     5641 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
     5701 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
     5761 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
     5821 attgctgccc tctggttatg tgtgggaggg ctaaggtacc tttaagacca atgacttaca
     5881 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     5941 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6001 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6061 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6121 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6181 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     6241 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6301 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6361 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6421 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6481 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     6541 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     6601 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     6661 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     6721 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6781 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6841 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6901 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6961 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7021 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     7081 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     7141 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     7201 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7261 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7321 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7381 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7441 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7501 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7561 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7621 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7681 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     7741 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7801 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7861 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7921 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7981 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8041 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8101 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8161 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8221 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8281 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8341 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8401 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8461 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8521 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8581 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8641 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8701 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8761 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8821 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8881 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8941 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9001 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     9061 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9121 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     9181 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     9241 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     9301 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     9361 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaa
//
