LOCUS       sequence-321906-        9845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human GLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 9845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC6-GLS-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     260..279
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             2487..2552
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2600..2717
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2766..3276
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3195..3213
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3285..3680
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3752..3985
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4057..4178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4094..4113)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4148..4167
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4218..4353
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4373..4392)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4374..4392)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4402..4419)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4402..4418)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4411..4433)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4560..5015
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4647..4666)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4857..4878
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5041..5145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5146..5214,5215..6006)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5364..5383)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6177..6765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6666..6685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6919..6936
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7053..7074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7089..7094,7095..7112,7113..7119)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7127..7143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7132..7154
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7151..7167
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7151..7167
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7186..7206
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7188..7206
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7234..7460
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7461..7641
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7688..7813
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8306..8539
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8724..8768
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        join(9057..9845,1..423)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca
       61 cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct
      121 tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg
      181 ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt
      241 acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt
      301 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
      361 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
      421 gacgaagcgc tagcgctacc ggtttaatta acggcggcat gatgcgcctg aggggatcag
      481 gtatgctcag agatcttctc cttcggagtc ctgcgggcgt gagcgctact ttgcgacggg
      541 ctcaaccgct ggttacactc tgtcggcgac ccaggggcgg cgggcgaccg gctgctgggc
      601 ctgccgccgc cgcgagactt catccatggt gggggggagg tggatggccc gctgaaccac
      661 tcgcgcgggg gcttagctca agcccaagcg agatcctgca ggagctggga aaaggctcta
      721 ctcacccgca accgggggta agcccacctg cggcaccggc ggccccaggg ccaaaggatg
      781 gaccgggaga aacagacgct ttcggtaaca gtgagggcaa ggagctggta gcatcaggcg
      841 agaataagat caagcaagga ctcctgcctt ctcttgagga cctgttgttt tacacaatag
      901 ccgaaggaca ggagaagatt ccggtacaca aatttataac cgcacttaag tccacaggat
      961 tgagaacgtc tgatcccaga ttgaaggagt gcatggacat gctgaggctg acccttcaaa
     1021 caacttccga cggtgttatg ctggataagg acttgttcaa gaagtgcgta caatctaaca
     1081 tcgtactttt gacgcaggcg tttagaagga aattcgttat tcccgacttt atgtcattca
     1141 ccagccatat agatgagctc tacgaaagtg ctaagaaaca gtctggaggg aaagtggcag
     1201 attacatacc acagctggcc aaatttagtc cagacctgtg gggcgtgagt gtatgtactg
     1261 ttgatggcca gcgacactct accggcgata caaaagtacc cttctgcctg cagtcttgtg
     1321 tcaaacctct taagtacgct atcgctgtca acgatctggg gacggagtac gttcatagat
     1381 atgtgggaaa ggagccatcc ggcctgcgat ttaataagct tttccttaac gaggacgata
     1441 aacctcataa tcccatggta aacgccggcg ccatagtcgt caccagcctc atcaagcaag
     1501 gtgtgaacaa cgctgaaaag ttcgactacg taatgcagtt cctgaacaaa atggcgggta
     1561 atgagtatgt aggtttctct aacgcgacct tccagtctga acgggaatcc ggcgaccgga
     1621 actttgcgat cggatactac ctgaaggaaa aaaaatgctt tccagaagga acagatatgg
     1681 tcggaatcct ggatttttac tttcaattgt gctctattga ggttacatgc gaatctgcga
     1741 gtgtcatggc cgcaactctt gcgaacggtg gattttgtcc gatcacggga gaacgggtct
     1801 tgtctcctga agcggtgaga aataccctct cattgatgca ttcttgtgga atgtatgatt
     1861 tctctgggca attcgccttt catgtggggc ttcccgctaa gtccggcgtg gctggcggga
     1921 ttctgttggt agtgccgaac gtaatgggaa tgatgtgctg gtcccccccg ctcgacaaaa
     1981 tgggaaattc tgtcaagggc attcacttct gtcatgatct tgtcagcctt tgtaatttcc
     2041 acaattacga taacttgcga cattttgcca agaaactgga tcctagaagg gagggagggg
     2101 atcagcgggt gaaatcagtc attaacctcc tctttgctgc ctacaccggc gatgttagtg
     2161 cactgcgaag gtttgccctt tccgcaatgg atatggaaca gcgggattac gactccagga
     2221 ccgctcttca cgtagctgct gcggagggcc acgttgaagt cgtaaaattc ctgctggagg
     2281 cgtgcaaagt aaatccattc cccaaagatc gctggaataa cactcccatg gacgaggctt
     2341 tgcatttcgg acaccacgac gtgttcaaaa ttctgcagga ataccaggtt cagtatacac
     2401 cccagggaga ttctgacaac ggaaaagaaa accagactgt tcataagaat ctcgatgggc
     2461 tcctgggagg cagcgcggcc gcaggcgact acaaagacca tgacggtgat tataaagatc
     2521 atgacattga ttacaaggat gacgatgaca agtaataaga attctcgacc tcgagacaaa
     2581 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     2641 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2701 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc
     2761 tcgagggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     2821 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     2881 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     2941 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     3001 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     3061 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
     3121 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     3181 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     3241 tccctcgttg accgaatcac cgacctctct ccccaggggg atccatggtt cctttgtctc
     3301 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg
     3361 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca
     3421 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
     3481 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
     3541 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca
     3601 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
     3661 gttatgtgtg ggagggctaa ggtaccttta agaccaatga cttacaaggc agctgtagat
     3721 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     3781 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     3841 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3901 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3961 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     4021 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     4081 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4141 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     4201 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4261 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     4321 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     4381 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     4441 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     4501 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     4561 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4621 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4681 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4741 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4801 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4861 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4921 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4981 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     5041 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5101 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5161 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5221 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5281 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5341 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5401 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5461 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5521 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5581 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5641 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5701 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5761 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5821 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5881 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5941 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6001 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6061 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7021 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7081 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7141 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     7201 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     7261 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     7321 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     7381 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     7441 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     7501 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7561 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7621 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     7681 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     7741 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     7801 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     7861 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     7921 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     7981 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     8041 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     8101 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cagcagctga
     8161 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     8221 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     8281 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     8341 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     8401 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     8461 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     8521 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     8581 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     8641 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     8701 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     8761 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     8821 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     8881 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     8941 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     9001 gattagtgaa cggatctcga cggtatcgat cacgagacta gcctcgagcg ggatctggcc
     9061 tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcgctgccac
     9121 gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc
     9181 ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg
     9241 ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa
     9301 gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt
     9361 atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg
     9421 gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc
     9481 ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag
     9541 ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc
     9601 aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc
     9661 gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct
     9721 aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg
     9781 tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg
     9841 gcggt
//
