LOCUS       sequence-321907-        8996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human METAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 8996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC6-METAP1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..8996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     260..279
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1638..1703
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    1751..1868
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1917..2427
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2346..2364
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2436..2831
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             2903..3136
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3208..3329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3245..3264)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3299..3318
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3369..3504
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3524..3543)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3525..3543)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3553..3570)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3553..3569)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3562..3584)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3711..4166
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3798..3817)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4008..4029
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4192..4296
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4297..4365,4366..5157)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4515..4534)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5328..5916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5817..5836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6070..6087
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6204..6225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6240..6245,6246..6263,6264..6270)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6278..6294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6283..6305
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6302..6318
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6302..6318
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6337..6357
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6339..6357
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6385..6611
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6612..6792
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6839..6964
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7457..7690
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7875..7919
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        join(8208..8996,1..423)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca
       61 cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct
      121 tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg
      181 ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt
      241 acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt
      301 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
      361 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
      421 gacgaagcgc tagcgctacc ggtttaatta aaggcagcat ggcggccgtg gagacgcggg
      481 tgtgcgagac agacggctgc agcagtgagg ccaagctcca gtgtcccact tgcatcaagc
      541 tgggcatcca gggatcctat ttttgttccc aggaatgttt taaaggaagt tgggctactc
      601 acaagttact acataagaaa gcaaaagatg aaaaggcgaa gcgagaagtg tcttcctgga
      661 ctgtggaagg tgatattaat actgacccat gggcaggtta tcgatatact ggtaaactca
      721 gaccacatta tccactgatg ccaacaaggc cagtgccaag ttatattcaa agaccagatt
      781 atgctgatca tcccttagga atgtctgaat ctgaacaggc tcttaaaggt acttctcaga
      841 ttaaattact ctcatctgaa gatatagaag ggatgcgact tgtatgtagg cttgctagag
      901 aagttttgga tgttgctgcc ggcatgatta aaccaggtgt aactactgaa gaaatagatc
      961 acgctgtaca cttagcatgt attgcaagaa attgctaccc ttctcccctg aattattata
     1021 atttcccaaa gtcttgttgt acctcagtga atgaagtcat ttgccatgga ataccagaca
     1081 gaaggccctt acaagaaggt gacattgtta atgtggatat cactctttat cgcaatggtt
     1141 atcatgggga cctgaatgag acattttttg ttggagaagt ggatgatgga gcacggaaac
     1201 ttgttcagac cacatatgag tgcctgatgc aagccattga tgcagtgaag cctggtgttc
     1261 ggtacagaga attgggaaac attatccaga agcatgccca agcaaatggg ttttcagttg
     1321 ttcgaagcta ttgtgggcat ggaatccaca agctttttca tacagctccc aatgtacccc
     1381 actatgctaa aaataaagca gttggagtga tgaagtcggg ccatgtattt acaattgagc
     1441 caatgatttg tgaaggcgga tggcaggatg aaacctggcc agatggttgg actgcggtga
     1501 caagagacgg aaagcggtct gctcagtttg agcacaccct cctggtcaca gacactggct
     1561 gtgaaatcct aacccggcga cttgacagtg cacggcctca cttcatgtct caatttggag
     1621 gcagcgcggc cgcaggcgac tacaaagacc atgacggtga ttataaagat catgacattg
     1681 attacaagga tgacgatgac aagtaataag aattctcgac ctcgagacaa atggcagtat
     1741 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     1801 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     1861 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg
     1921 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     1981 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2041 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2101 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     2161 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     2221 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     2281 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     2341 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     2401 gaccgaatca ccgacctctc tccccagggg gatccatggt tcctttgtct caagaagaat
     2461 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     2521 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     2581 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     2641 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     2701 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     2761 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     2821 gggagggcta aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     2881 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     2941 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     3001 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     3061 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     3121 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     3181 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     3241 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     3301 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     3361 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3421 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     3481 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     3541 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     3601 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     3661 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     3721 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3781 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3841 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3901 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     3961 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4021 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4081 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4141 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     4201 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4261 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4321 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4381 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4441 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4501 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4561 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4621 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4681 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4741 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4801 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4861 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4921 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4981 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5041 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5101 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5161 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5221 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5281 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5341 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5401 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5461 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5521 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5581 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5641 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5701 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5761 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5821 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5881 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5941 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6001 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6061 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6121 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     6181 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     6241 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     6301 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     6361 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     6421 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     6481 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     6541 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     6601 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6661 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6721 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6781 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     6841 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     6901 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     6961 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     7021 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     7081 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     7141 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     7201 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     7261 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcagcagctg atcttcagac
     7321 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     7381 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     7441 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     7501 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     7561 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     7621 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     7681 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     7741 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     7801 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     7861 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     7921 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     7981 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     8041 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     8101 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     8161 acggatctcg acggtatcga tcacgagact agcctcgagc gggatctggc ctccgcgccg
     8221 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     8281 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     8341 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     8401 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     8461 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     8521 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     8581 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     8641 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     8701 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     8761 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     8821 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     8881 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     8941 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggt
//
