LOCUS       sequence-32192-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Wang Q, Mora-Jensen H, Weniger MA, Perez-Galan P, Wolford C, Hai T, 
            Ron D, Chen W, Trenkle W, Wiestner A, Ye Y
  TITLE     ERAD inhibitors integrate ER stress with an epigenetic mechanism to 
            activate BH3-only protein NOXA in cancer cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Feb 17. 106(7):2200-5.
  PUBMED    19164757
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Feb 17. 106(7):2200-5."
COMMENT     Sequence Label: pRK-ATF4 248-351
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(274..291)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     CDS             complement(360..383)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      383..392
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(432..451)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(464..482)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(465..482)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(692..716)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(713..916)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(742..762)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tagaataggg ccctctagat gcatgctcga gcggccgcct aggggaccct tttcttcccc
       61 cttgccttgc ggacctcttc tatcaaatct ttcaggtact ggatctcctt ggccagggaa
      121 tccgccctct cttttagagc ctcgttcttc ttttccagct ctttgcactc accagtaaga
      181 gcctcctgct ccgccctctt cttctggcgg tacctagtgg ctgctgtctt gttttgctcc
      241 atttttttca gcttcttatc cagtttctca ccctttactt ttgctgctac catcttctct
      301 ccaggaggat cgtaaggttt gggacgggca gacccacaga gaacacctgg ggtcgacccc
      361 ttgtcatcgt cgtccttgta gtccatggcg gcaagcttag aaccgaggtg cagttggacc
      421 tgggagtgga cacctgtgga gagaaaggca aagtggatgt tattctatag tgtcacctaa
      481 atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct aacgaagcca
      541 agggggtggg cctatagact ctataggcgg tacttacgtc actcttggca cggggaatcc
      601 gcgttccaat gcaccgttcc cggccgcgga ggctggatcg gtcccggtgt cttctatgga
      661 ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc
      721 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
      781 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
      841 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
      901 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
      961 gaatccaagc
//
