LOCUS       sequence-321929-       14793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium
            smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. 
            marinum and recX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14793)
  AUTHORS   Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC
  TITLE     A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient 
            Genome Editing in Mycobacterium tuberculosis.
  JOURNAL   mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 
            10.1128/mBio.02364-19.
  PUBMED    31992616
REFERENCE   2  (bases 1 to 14793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2020-01-28"; volume: "11(1). pii: mBio.02364-19. doi"; pages:
            " 10.1128/mBio.02364-19"
COMMENT     Sequence Label: pNHEJ-Cas12a-recX
FEATURES             Location/Qualifiers
     source          1..14793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    4356..4389
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      4455..5043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4944..4963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8257..8268
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11903..11922)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             11914..12729
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(11987..12006)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(13030..13076)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    14128..14161
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(14178..14793,1..8))
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     14709..14728
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ctagacattg cgaagtgatt cctccgatcc gtggcaggag ggtcgcctgc gtcggcaact
       61 cgcccaggct acacgagagc cctcgactgc gaaaatgccg accaggcacc accgcactcc
      121 aggcttaacc cggtgcgacg atccaatatt aatattggat cgtcggcacc gtcacggccg
      181 tgggaggcgg cacgatccgc gacgtgatga tcggccgcat ccccacggtg ctgcgcagtg
      241 agctctacgc catcccggcg ttgatctgtg cgttcgcacg cacaggcccg gtgtgagaag
      301 ggtctctgac gagcgggaga actccctatc agtgatagag tttgtcctcc ctatcagtga
      361 tagataggct ctgggagtac ccgtgtgtac gaccagcacg gcatacatca tttcgacgcc
      421 gagagattcg ccgcccgaaa tgagcacgat ccatgtcgat ctaccaagag ttcgtgaata
      481 agtatagcct gagcaagacc ttgcggttcg agttgatccc gcagggcaaa accttggaaa
      541 acattaaggc gcgcgggctg atcctggatg atgagaagcg ggccaaggac tataaaaagg
      601 ccaagcagat tatcgataaa tatcatcagt tcttcatcga ggaaatcctg tcgagcgtgt
      661 gcatcagcga agacttgttg cagaactata gcgacgtgta cttcaaattg aaaaagtcgg
      721 acgacgacaa tctgcaaaaa gacttcaagt cggccaaaga caccatcaag aaacagattt
      781 cggagtacat caaggactcg gaaaagttca aaaacctgtt caaccagaac ttgatcgacg
      841 cgaaaaaggg ccaggaaagc gatttgatct tgtggctgaa gcagtcgaag gataatggca
      901 ttgagttgtt caaggccaac tcggacatta cggacattga cgaggccctg gaaatcatta
      961 aatcgtttaa gggctggacc acgtacttca agggcttcca tgagaaccgg aaaaacgtgt
     1021 attcgtcgaa cgacattccc acgtcgatca tctaccggat cgtcgatgac aacttgccca
     1081 agttcttgga aaataaagcg aaatacgaga gcttgaagga caaagcgccg gaggccatta
     1141 actatgagca gatcaagaag gacctggccg aagagctgac ctttgacatt gactacaaga
     1201 ccagcgaggt caatcagcgg gtcttctcgt tggatgaggt ctttgaaatc gccaatttca
     1261 acaactattt gaaccagagc ggcatcacca aatttaatac cattatcggc gggaagttcg
     1321 tcaacggcga gaacacgaag cggaagggga tcaacgaata catcaatctg tactcgcagc
     1381 agatcaacga caagaccctg aaaaagtaca agatgagcgt gctgttcaag caaattctga
     1441 gcgacaccga gagcaagtcg ttcgtcattg acaagctgga ggatgacagc gatgtggtca
     1501 ccacgatgca gtcgttttac gagcaaatcg cggccttcaa gaccgtggag gaaaagagca
     1561 tcaaggaaac cctgagcctg ctgttcgatg atctgaaggc gcagaagttg gatctgtcga
     1621 aaatctactt caagaacgat aagtcgctga ccgacctgag ccaacaggtc ttcgatgatt
     1681 acagcgtcat cgggaccgcc gtcctggagt acatcaccca gcagattgcg cccaaaaacc
     1741 tggacaatcc ctcgaaaaaa gagcaggagc tgattgccaa gaagaccgag aaggcgaagt
     1801 acttgtcgct ggaaaccatc aaactggcgc tggaggagtt caacaagcac cgcgacattg
     1861 ataaacaatg ccggttcgag gagattctgg ccaattttgc cgccattccc atgatcttcg
     1921 atgagatcgc gcagaataaa gacaacctgg cgcaaatctc gatcaaatat cagaatcagg
     1981 gcaaaaaaga cctgttacaa gcctcggccg aagacgacgt gaaagccatc aaggacctgt
     2041 tggatcagac gaacaacttg ttgcacaagc tgaaaatttt ccacatcagc cagagcgagg
     2101 acaaggccaa catcttggac aaagacgagc acttctactt ggtgttcgaa gagtgctact
     2161 tcgaattggc caacatcgtc cccctgtaca ataagattcg caattatatt acgcagaagc
     2221 cctatagcga cgagaaattc aagttgaatt ttgaaaattc gaccctggcg aatggctggg
     2281 acaagaacaa agagcccgac aacaccgcca tcttgttcat caaggatgat aaatattatc
     2341 tgggggtgat gaacaaaaag aataacaaga tctttgatga caaggccatc aaggagaata
     2401 agggcgaggg gtacaagaaa atcgtctaca aactgttgcc cggcgccaac aagatgttgc
     2461 cgaaggtctt cttcagcgcg aagagcatta aattctataa ccccagcgag gacatcctgc
     2521 gcatccggaa tcattcgacc cacacgaaga atggcagccc gcaaaagggc tacgagaagt
     2581 tcgagtttaa catcgaggat tgtcggaaat tcatcgactt ctataaacag tcgatcagca
     2641 agcaccccga gtggaaggat ttcggcttcc gcttcagcga tacccaacgc tacaacagca
     2701 tcgacgagtt ttatcgggaa gtggaaaacc aggggtacaa gttgaccttc gagaacattt
     2761 cggagtcgta cattgactcg gtcgtcaacc aagggaaact gtacctgttc caaatctaca
     2821 ataaggactt cagcgcgtac tcgaaagggc ggcccaactt gcacaccctg tattggaagg
     2881 ccttgtttga cgagcggaac ttacaagacg tcgtctataa actgaacggg gaggcggagc
     2941 tgttttaccg caagcagtcg atcccgaaaa agattaccca cccggcgaag gaggcgatcg
     3001 ccaacaaaaa caaggacaac ccgaagaaag agtcggtctt cgaatacgat ctgatcaaag
     3061 acaagcgctt taccgaggac aagttctttt tccattgtcc gatcaccatc aattttaaga
     3121 gcagcggcgc gaacaagttc aacgacgaga tcaacctgct gttgaaagaa aaggccaatg
     3181 atgtgcacat cctgtcgatc gaccgggggg aacggcacct ggcctactac accctggtgg
     3241 acggcaaggg caacatcatc aagcaggata ccttcaatat cattgggaac gaccggatga
     3301 aaacgaacta ccacgacaag ctggcggcca tcgaaaagga tcgcgacagc gcccgcaagg
     3361 actggaagaa gattaacaat atcaaggaga tgaaggaggg gtacttgagc caggtcgtgc
     3421 acgagatcgc caagctggtc attgagtaca acgcgattgt ggtctttgaa gacctgaact
     3481 ttggcttcaa acgggggcgg ttcaaggtcg agaagcaggt gtatcagaag ctggaaaaaa
     3541 tgttgatcga gaagctgaac tatctggtgt tcaaagacaa cgagtttgac aaaacggggg
     3601 gcgtcctgcg ggcctaccag ttgaccgccc ccttcgaaac cttcaagaaa atgggcaagc
     3661 agacgggcat catctactac gtcccggcgg gctttacgag caagatctgt ccggtgaccg
     3721 gcttcgtcaa ccagctgtat ccgaagtatg agagcgtgtc gaaaagccag gagtttttct
     3781 cgaagttcga taaaatctgt tataacctgg ataaggggta cttcgaattt tcgttcgatt
     3841 acaaaaactt cggggacaag gcggccaagg ggaaatggac gatcgccagc ttcggctcgc
     3901 gcctgattaa ctttcgcaac tcggacaaga accataactg ggacacccgg gaagtgtacc
     3961 cgaccaaaga attggaaaaa ctgttgaagg actatagcat cgagtacggg cacggggagt
     4021 gcatcaaagc cgccatctgc ggggaaagcg acaagaagtt ttttgcgaag ctgaccagcg
     4081 tgctgaatac gatcttacaa atgcgtaatt ctaagaccgg caccgagctg gactacctga
     4141 tctcgccggt ggcggatgtc aacggcaact tcttcgacag ccgccaggcc ccgaagaaca
     4201 tgccgcagga cgcggatgcg aatggcgcgt accacatcgg gctgaaaggg ctgatgctgc
     4261 tggggcggat caagaacaac caggagggga agaagctgaa tctggtgatt aagaacgagg
     4321 aatatttcga gttcgtccaa aatcgcaaca attgaataac ttcgtataat gtatgctata
     4381 cgaagttatt aattctgcag agtcctacta gttccatgag cgtcagaccc cgtagaaaag
     4441 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4501 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4561 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     4621 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4681 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4741 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4801 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     4861 acgcttcccg aggggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4921 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4981 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5041 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5101 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     5161 gctgataccg ctcgccgcag ccgaacgacc gagcgcaacg cgtgagccca ccagctccgt
     5221 aagttcgggc gctgtgtggc tcgtacccgc gcattcaggc ggcagggggt ctaacgggtc
     5281 taaggcggcg tgtacggccg ccacagcggc tctcagcggc ccggaaacgt cctcgaaacg
     5341 acgcatgtgt tcctcctggt tggtacaggt ggttgggggt gctcggctgt cgctggtgtt
     5401 ccaccaccag ggctcgacgg gagagcgggg gagtgtgcag ttgtggggtg gcccctcagc
     5461 gaaatatctg acttggagct cgtgtcggac catacaccgg tgattaatcg tggtctacta
     5521 ccaagcgtga gccacgtcgc cgacgaattt gagcagctct ggctgccgta ctggccgctg
     5581 gcaagcgacg atctgctcga ggggatctac cgccaaagcc gcgcgtcggc cctaggccgc
     5641 cggtacatcg aggcgaaccc aacagcgctg gcaaacctgc tggtcgtgga cgtagaccat
     5701 ccagacgcag cgctccgagc gctcagcgcc cgggggtccc atccgctgcc caacgcgatc
     5761 gtgggcaatc gcgccaacgg ccacgcacac gcagtgtggg cactcaacgc ccctgttcca
     5821 cgcaccgaat acgcgcggcg taagccgctc gcatacatgg cggcgtgcgc cgaaggcctt
     5881 cggcgcgccg tcgatggcga ccgcagttac tcaggcctca tgaccaaaaa ccccggccac
     5941 atcgcctggg aaacggaatg gctccactca gatctctaca cactcagcca catcgaggcc
     6001 gagctcggcg cgaacatgcc accgccgcgc tggcgtcagc agaccacgta caaagcggct
     6061 ccgacgccgc tagggcggaa ttgcgcactg ttcgattccg tcaggttgtg ggcctatcgt
     6121 cccgccctca tgcggatcta cctgccgacc cggaacgtgg acggactcgg ccgcgcgatc
     6181 tatgccgagt gccacgcgcg aaacgccgaa tttccgtgca acgacgtgtg tcccggaccg
     6241 ctaccggaca gcgaggtccg cgccatcgcc aacagcattt ggcgttggat cacaaccaag
     6301 tcgcgcattt gggcggacgg gatcgtggtc tacgaggcca cactcagtgc gcgccagtcg
     6361 gccatctcgc ggaagggcgc agcagcgcgc acggcggcga gcacagttgc gcggcgcgca
     6421 aagtccgcgt cagccatgga ggcattgcta tgagcgacgg ctacagcgac ggctacaacc
     6481 ggcagccgac tgtccgcaaa aagcggcgcg tgaccgccgc cgaaggcgct cgaatcaccg
     6541 gactatccga acgccacgtc gtccggctcg tggcgcagga acgcagcgag tggctcgccg
     6601 agcaggctgc acgccgcgaa cgcatccgcg cctatcacga cgacgagggc cactcttggc
     6661 cgcaaacggc caaacatttc gggctgcatc tggacaccgt taagcgactc ggctatcggg
     6721 cgaggaaaga gcgtgcggca gaacaggaag cggctcaaaa ggcccacaac gaagccgaca
     6781 atccaccgct gttctaacgc aattggggag cgggtgtcgc gggggttccg tggggggttc
     6841 cgttgcaacg ggtcggacag gtaaaagtcc tggtagacgc tagttttctg gtttgggcca
     6901 tgcctgtctc gttgcgtgtt tcgttgcgcc cgttttgaat accagccaga cgagacgggg
     6961 ttctacgaat cttggtcgat accaagccat ttccgctgaa tatcggggag ctcaccgcca
     7021 gaatcggtgg ttgtggtgat gtacgtggcg aactccgttg tagtgcctgt ggtggcatcc
     7081 gtggcgcggc cgcggtaccc aagaagcaca gtgagtcgcg gatcgatggc gggcgcggta
     7141 tcggcgccgc caacgtcacc tcgtcgagag ccaccacctc actcggtgag cgcaagacgt
     7201 tttcaccggc ttcgcgtaat ccgtcggcgc cgcgcttgat caaatcgagc agggcgactc
     7261 ccggcgccat ggcgtagatc gaaccgtcgg acaacacccc gacacgctca ccgtccgcac
     7321 ttcgatacgt cacccatttc attcgcgcac cacctcactc gggtttttgt ccgggcgcaa
     7381 cccacgccag ctggattgcc gcaatcggtc atcgggggtc cactcgctat agcgcacctc
     7441 cccgaccagc gtcggcgcga cgaacgtaac acccttggcc tcgcgcgccg gaagcgcagc
     7501 gtcgaatggg gattcgtcgg tatgcagcgg cgccagcgtc ttcttgaggt tggccaggtc
     7561 acgttcggtg aagccggttc caacacggcc ggcgaagtgc aaaccgctcg ggccgggaat
     7621 gcccatgagc aacgatccaa tcccgctgct gcgcccaccc tccccaactc gccaaccgcc
     7681 gatgacgact tcctgggtgt tccagtgttt gtccttgatc cacgccgacg agcgccgccc
     7741 gggttgatag ccggagtcgc ggcgcttggc aatgactccc tcccaaccgc gcttgtgcga
     7801 gtaggccagc gcctcggcgc cgtcaccggg caacaactcc ggaacgatca gattgcccgc
     7861 actgcccagg gtttccagta gcttgcgccg gtcgcggtat ctggcccgca gtagcgagcg
     7921 accgtccaga aagagcaagt cgaacgccca gaactcgatg cgggtggcgc gcacccggtt
     7981 ctgcatctcg ttgaagctgg gcactccgga ggagtcgagg gccaccaatt ccccgtcgag
     8041 caccacgtgg tgatcagcga gatcggcggc caatgaacgt aattgggggt attccgcggt
     8101 gacgtcacgt ccgctgcggg agcgcagtcg cagcgcgcca tggtcggctt cgaccagcag
     8161 ccggtagccg tcccacttgc cttcgaacgc ccactgaccc gactcgagac ctgccaccga
     8221 accgtgggag gtgagcatgg gcgcgagggt gtcgaaatcg aaggccgtct ggtccttcat
     8281 gcgatgcgcc agccattggt ccccgttggt ctgaatcagc gcgtaccgcc ccgagattcg
     8341 actgccgtgc aggttgacaa tcacctcccc cttttccgaa ccgtttctcg agtcgtcgcg
     8401 gaacttctcg gtgtcatagg tgccggaatc ccagatgatc accttgccgg cgccgtactc
     8461 ccctttcggg atgttgccct cgaacgtcgc gtattccagc ggatgatctt cggtgtgcac
     8521 ggccaggtgg ttcaccgacg tggtttccgg caggtttttc ggcaccgccc atgacaccag
     8581 taccccatca cgctccagcc tgaagtcgta atgcagacgg cgcgcgtgat gctcttggat
     8641 cacgaaggta ttaccctgcc cgacaacggg tctggctcgg ggcaccggct ccggcgtttt
     8701 cgacgcgttg cgcatgctgc gatatttgag cagccggtcg tcgccgggga ggtccgcgtc
     8761 cagcgccgca atcagatcgc catcgcgggt gaggcgggca agcacctcgt cgtaggtcag
     8821 ctggcgcagc cccgggtcgt cgagctcctc ccagctgcgt ggtgcggcga cggtcggttg
     8881 ttcgcgccca cgcagcgaat acggcgcgat ggtggtcttc gaaccgctgt tttggctcca
     8941 gtccaggaac actttcccgg cccgcagact cttggtcatg gtcgcggtga ccaacgccgg
     9001 catcaccgtt tccagttgct gcgcgacccg tttggccaac accgtggcgc ccttgctgct
     9061 caccggctcg tcgagcgggg tgtagaggtg caggcccttg ctaccgctgg tgagcgggag
     9121 ggtactcagg ccgatgtcgg cgagcagatc gcgcaccgcc cgcgccacct gcgcgagttg
     9181 ggccatcgtc acgccctcgc ccgggtccag gtcgaacacc aatcgggtgg ccggcccggg
     9241 cgtgaggatt tcgcctttcg acgtccactc ggcaacgaac cgccactgcg ggacgtgcac
     9301 ctcgagggcg gcctgttgcg cgatccaggc cagcccggtg gcgctgtcga tgatcggata
     9361 ggtcgtcgtt cccgaccggt gcaccacgct ggcacgcggc agccagtccg gcgccgacga
     9421 cgccagctgc ttttcgaaaa aggacgcctg atcgacgccg ttgggccagc gtttgcgagt
     9481 cgccgggcgc ccggcgatat gcgggatcat cacctcggcg atgccggtgt agtaaccgaa
     9541 gacgtcagac ttggtggttc cggtagccgg gtacagcacc ttgtccgcgt tggtcagtgt
     9601 cacctgcgga ccattcgcct gtccaggagg ccaggcctgc gccgaaccca tatggccaac
     9661 gtaacctgtc gccatggcac gtgggctggt gatcggtgaa gccctgatcg acatcgtcga
     9721 tgggccggac ccggccgagt atgtcggggg cagccccctc aacgtcgcgg tcggactggc
     9781 cagattgggc cgcgacgtcg atctgctgac ccacatcggc cgcgacgccc gcggacgacg
     9841 gatcgccgag tacattgaat cctcaggtgt acaacttgtt tcgggaagcc agacggctga
     9901 tcggacgccc accgcgacgg ccaggatcgg ccccgacggc tcggccacct acgcgttcga
     9961 cctggaatgg cagatacccg acacaccccc cgtggcgccg ccgcttctcg tgcacaccgg
    10021 ttccatcgcc gccgcgcggg aaccaggatg cttggcggtc gcggcgctgc tcgacgccta
    10081 tcgggccgcg gccacggtga gcttcgatcc caatgtgcgt ccgtcgctga gcgccgaccc
    10141 ggacctgact cgggaacgca tccaacgcct cgtcgagcgc agcgacatca tcaaggccag
    10201 tgccgaagac ttgcactgga tcgaccccac ccagcctccg gagcagaccg cgcgggcctg
    10261 gctggcctgc ggcccggcga ttgtcgcgct gacgttgggc gatcagggcg cagtggcgtt
    10321 ctgcgcggcc ggcccggcaa gtgtgcccgc ccagccggtt cgcgtggtcg ataccgtcgg
    10381 cgccggagac gcgttcatgg ccggtctgct cgacacgctg tgggagcagg gattgttagg
    10441 tgccgaccgg cgcaccgagt tgcgcaagat cggcgtgtcg gcgctgacct ctgcactcga
    10501 agtcgcggcc ctgacctcgg cgttgaccgt tgcccgtgcc ggcgccgatc tgccctatcg
    10561 ggccgacctg cggcagtcgc gatgaccacc gcacgcccgc aatcgggccc ggcgagggtg
    10621 cacgaaatgc cgcgtgatgc ggcgtgtcgc cgtgccgaca cgcacgctcg cgagctgacc
    10681 ggggctgggg caggcagcag gactccggtg ccgcggcgca ctgttaaccg acccgggttc
    10741 tgggcatact gactccatgc gctccatctg gaagggttca atcgctttcg ggctggtgaa
    10801 tgtgccggtc aaggtgtaca gcgccactca ggaccacgac atcaagttcc atcaggtgca
    10861 cgccaaggac aacggacgca tccggtacaa gcgggtatgc gaggtctgtg gcgaggtggt
    10921 cgaatactcc gacctggccc gcgccttcga atccgacgat ggccagatgg tgatcatcaa
    10981 cgacgacgac ctcgccacgt tgcccgaaga tcgcagccgc gaaatcgagg tgctggaatt
    11041 cgtcccagcc gaagaagtcg atccgatgat gattgaccgc agctacttcc tggagcccga
    11101 ctcgaagtcc tctaaatcgt atgtgctgct ggccaagacg ctcgccgaga cggagcggat
    11161 ggcgatcgtg catttcacgc tgcgcagcaa gacgcggctg gcggcgttgc gggtcaagga
    11221 cttcggcaag cgcgaggtca tggtggtgca taccctgttg tggccagacg agatccgcga
    11281 cccggatttc ccggtgctgg acaaggaggt ggagatcaaa cccgcggagc tcaagatggc
    11341 cggccaagtg gtggagtcga tggcagacga cttcaacccg gaccgctacc gcgacaccta
    11401 tcaggagcaa ctgcaggagc tgatcgacgc caaactcgaa ggcggggaag catttaccgc
    11461 cgaagaacaa cccgcagagc tggacgagac cgaagatgtc tccgacctgc tggccaagct
    11521 ggaggcgagc gtgaaagcgc gttcgggcga cgggaagtca gcggcgaaga agagcaccgc
    11581 cgaaaaggcg ccggccaaga aggcccgggc caaacaagct gccaagtcct gaccagcgcc
    11641 cgacttgttc acatatgctc gtcgtgccgc cgagaggagt ttttgcgtga agtccaaccg
    11701 atctggcgtc gtgctcagcc tgctgctcgc cagcacattg gcgttgtcgg catgcggagg
    11761 ccattccagc aatacctcgt ccacctgacg tcgggggggg gggaaagcca cgttgtgtct
    11821 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
    11881 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
    11941 tgctcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
    12001 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gccccatgcg
    12061 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
    12121 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
    12181 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
    12241 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
    12301 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
    12361 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
    12421 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataatctttt gccattctca
    12481 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
    12541 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
    12601 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
    12661 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
    12721 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
    12781 cgggacggcg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc
    12841 atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt
    12901 ccacctacaa caaagctctc accaaccgtg gctccctcac tttctggctg gatgatgggg
    12961 cgattcaggc ctggtatgag tcagcaacac cttcttcacg aggcagacct cactagtggc
    13021 catgatggca taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
    13081 ctggcagtcg atcgtacgct agttaactac gtcgacagaa atattggatc gtcggcaccg
    13141 tcacggccgt gggaggcggc acgatccgcg acgtgatgat cggccgcatc cccacggtgc
    13201 tgcgcagtga gctctacgcc atcccggcgt tgatctgtgc gttcgcacgc acaggcccgg
    13261 tgtgagaagg gtctctgacg agcgggagaa cccacccggg gtgggcgagt ttgtcctgcg
    13321 tgtgctcggt cgagtaggct ctgggagtac ccgtgtgtac gaccagcacg gcatacatca
    13381 tttcgacgcc gagagattcg ccgcccgaaa tgagcacgat ccgcatgctt ataacagaaa
    13441 ggaggttaat aatgacgtcc tcccggcccc ggttgacttc tgagccgtcg gacgtccccg
    13501 acggcgagca ggcgcaggat cctcgcaccc gtgaggagca ggccaagaac gtctgcctgc
    13561 gcctgctcac cgtgcgggca cgtacccggg ccgagctcga aacccagctg accaagcgcg
    13621 gctatcccga cgacgtcagc gccagggttc tcgaccggct caccgaggtc ggcctgatcg
    13681 acgatgagga cttcgccgag cagtgggtgc gttcacgcca cctcaacgcc ggaaaaggca
    13741 agcgcgcctt ggctgtcgag ctccgtaaga agggcgtgga cgacgaggtg atctcgtcgg
    13801 ctctggccga tctcgacccg gccgccgagc gtcaacgggc cgagcagttg gtgcgcgaca
    13861 agttgcgccg cgaacgtctc gacggcacac cggaaaacga cgtcaaggtc acccgcaggc
    13921 tcgtgggcat gctggcgcgc cgcggataca accagtcgat ggcctacgac gtggtcagcg
    13981 tcgagttggc caacgagcgg gagcgccggc gggtctagta acgccgaaaa ccccgcttcg
    14041 gcggggtttt gccgcggtct gcgttgtcgg gaagatttaa aataaaaaag gggacctcta
    14101 gggtccccaa aagcttggcg tgaattcata acttcgtata atgtatgcta tacgaagtta
    14161 ttaattgccc atggtcatta agacccactt tcacatttaa gttgtttttc taatccgcag
    14221 atgatcaatt caaggccgaa taagaaggct ggctctgcac cttggtgatc aaataattcg
    14281 atagcttgtc gtaataatgg cggcatacta tcagtagtag gtgtttccct ttcttcttta
    14341 gcgacttgat gctcttgatc ttccaatacg caacctaagg taaaatgccc cacagcgctg
    14401 agtgcatata acgcgttctc tagtgaaaaa ccttgttggc ataaaaaggc taattgattt
    14461 tcgagagttt catactgttt ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg
    14521 cgatgactta gtaaagcaca tctaaaactt ttagcgttat tacgcaaaaa atcttgccag
    14581 ctttcccctt ctaaagggca aaagtgagta tggtgcctat ctaacatctc aatggctaag
    14641 gcgtcgagca gggctcgctt attttttaca tgccaataca atgtaggctg ctctacacca
    14701 agtttctggg cgagtttacg ggttgttaaa ccttcgattc cgacctcatt aagcagctct
    14761 aatgcgctgt taatcacttt acttttatct aat
//
