LOCUS       sequence-321930-       13048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe 
            harbor site. This is an all-in-one doxycycline-inducible construct, 
            enabling iPSC differentiation into peripheral sensory neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13048)
  AUTHORS   Nickolls AR, Lee MM, Espinoza DF, Szczot M, Lam RM, Wang Q, Beers J,
            Zou J, Nguyen MQ, Solinski HJ, AlJanahi AA, Johnson KR, Ward ME, 
            Chesler AT, Bonnemann CG
  TITLE     Transcriptional Programming of Human Mechanosensory Neuron Subtypes 
            from Pluripotent Stem Cells.
  JOURNAL   Cell Rep. 2020 Jan 21;30(3):932-946.e7. doi: 
            10.1016/j.celrep.2019.12.062.
  PUBMED    31968264
REFERENCE   2  (bases 1 to 13048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.12"; journalName: "Cell Rep"; date: 
            "2020-01-21- 21"; volume: "30"; issue: "3"; pages: "932-946.e7"
COMMENT     Sequence Label: pUCM-CLYBL-NGN2-BRN3A
FEATURES             Location/Qualifiers
     source          1..13048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     266..285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1391..1409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1427..1445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1463..1481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1499..1517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1535..1553
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1571..1589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1607..1625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1670..1694
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2620..2673
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2977..3003
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     polyA_signal    3962..4083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3999..4018)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4053..4072
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4132..4148)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4132..4148)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4145..4167)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4156..4172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4180..4186,4187..4204,4205..4210))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4225..4246
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4363..4380)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4672..4691
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    4690..4745
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     4791..4810
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(4901..5647)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5679..5698)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5706..6723
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5760..5782)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6725..7002)
                     /label=chicken beta-actin promoter
     enhancer        7004..7383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    7389..7613
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7602..7619
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    7635..7668
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     8322..8341
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(8342..8395)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(8417..9127,9128..9130,9131..9133))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8417..9127,9128..9130,9131..9133))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8459..8480)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8808..8827
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9067..9088
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      9130..9139
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             9167..9435
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(9448..9659)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    9692..9783
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(9724..9743)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    9797..9845
                     /note="synthetic polyadenylation signal"
     CDS             complement(10605..10718)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             complement(10800..10853)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    10877..10902
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    10903..10936
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(11794..11862,11863..12654)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12012..12031)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(12825..13048,1..365)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
       61 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc gaattcgccc ttagagcaga
      421 gccaggaacc cctgtaggga aggggcagga gagccagggg catgagatgg tggacgagga
      481 agggggacag ggaagcctga gcgcctctcc tgggcttgcc aaggactcaa acccagaagc
      541 ccagagcagg gccttaggga agcgggacgg ctctgttgga gagtccagta ttgaattagt
      601 gatagataca cagaaaaaaa gcaaattgaa taggtgaatg gataaacaaa ctgtggaaca
      661 tccagacaat gaaatattca ccaataaaaa gaaatgagct atcaagccac attggctatg
      721 cccttcacat ttaaggagga accttaaatg catattgcca aattttaaaa gctagtctga
      781 aaatgctaca gactgtatga ttccaattat atgacatttt agaaaaggca aaactataga
      841 gatcagtggt tgccagcggc tcatggaggc aggatgaatt ggtggaacac agggatgctt
      901 tcagggtaat gaaaatatct tgtgtgatcc tgtaataatg gatacgtggc ataatgcatt
      961 tgttaaaacc tgtaaactag ccaggtatag tggctttttt gcacctatag tctcagctac
     1021 ttggtaggct gagacaggaa gatcacttga gcccaggact tcaagaccag cctgggtaat
     1081 atagtaagac cccatctcta ttttttaaag aaattaagaa agaaaagaaa cccatcaact
     1141 gtataacaca aagaatcgta atgtaaacta cagtcctgag taatgaaaca tttttaaaga
     1201 aataaataaa ttcaaggcaa ttattaactt caggaaaaac agtaaagtaa acataagtat
     1261 acaaatcaaa catggctcag ttgtgaaaat atttacaagg tcactttaag taacattgaa
     1321 tgttgatata atcccaaaat atatttatgt tggaaggatg aggagatatc tccaccccac
     1381 agtggtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat
     1441 agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc
     1501 cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc
     1561 tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat
     1621 agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag
     1681 tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc aactttccgt
     1741 accacttcct accctcgtaa acttaaggtt aattaagcca ccatgttcgt gaagtccgag
     1801 acactggaac tgaaagagga agaggacgtg ctggtgctgc tgggctctgc ctctcctgct
     1861 ctggctgctc tgacacccct gagcagcagc gccgacgagg aagaagaaga ggaacctggc
     1921 gctagcggcg gagccagaag gcagagaggc gctgaagctg gacagggcgc aagaggcgga
     1981 gtggctgctg gcgcagaagg atgcagacct gccagactgc tgggcctggt gcacgactgc
     2041 aagagaaggc cctctagagc cagagccgtg tccagaggcg ccaagacagc cgagacagtg
     2101 cagcggatca agaaaaccag acggctgaag gccaacaacc gcgagcggaa ccggatgcac
     2161 aacctgaacg ccgccctgga cgccctgaga gaggtgctgc ctaccttccc cgaggacgcc
     2221 aagctgacca agatcgaaac cctgagattc gcccacaact acatctgggc cctgaccgag
     2281 actctgcggc tggccgatca ttgtggcggc ggaggcggag gactgccagg cgctctgttt
     2341 tctgaagccg tgctgctgtc tcctggcgga gcttctgctg ccctgtctag cagcggcgat
     2401 tctcctagcc ctgccagcac ctggtcctgc accaatagcc cagcccctag ctccagcgtg
     2461 tccagcaatt ccaccagccc ttacagctgc accctgagcc ctgcttctcc agccggcagc
     2521 gacatggact actggcagcc tcccccccct gacaaacatc gctacgcccc ccatctgcct
     2581 atcgcccggg attgcatcat gcacagcaga ggctctggcg agggcagagg cagcctgctg
     2641 acatgtggcg acgtggaaga gaaccctggc cccggatcca tgatgtccat gaactccaaa
     2701 cagccacact ttgccatgca cccaactctg cctgagcata agtacccatc cctgcattcc
     2761 tcctccgagg caatcaggag ggcttgtctg cctactcctc ctctccagag caacctgttc
     2821 gcttctctgg acgaaacact gctcgcaaga gccgaagccc tcgcagccgt ggacattgct
     2881 gtttctcagg gcaagtccca tcctttcaaa cccgacgcca cctaccacac catgaatagc
     2941 gtcccatgca ccagcacctc cactgtccca ctcgctcatc atcatcacca tcaccaccac
     3001 caccaggcac tggagcctgg tgatctcctc gaccacatct cctctccatc cctggctctg
     3061 atggctggag ctggaggtgc tggagcagct gcaggtggag gtggagccca cgacggtcca
     3121 ggaggaggtg gaggaccagg aggaggagga ggacctggag gtggtcctgg tggtggagga
     3181 ggaggaggac caggaggagg tggaggagga ccaggaggag gactcctcgg aggaagcgct
     3241 caccctcatc ctcacatgca cagcctgggt cacctgtccc accctgctgc tgctgctgct
     3301 atgaacatgc cttctggact gcctcaccct ggcctggttg cagcagctgc tcatcacggt
     3361 gcagctgctg cagctgcagc tgctgctgca ggtcaggtcg ctgctgcctc tgctgcagct
     3421 gccgtcgttg gagctgctgg actggcctcc atctgcgatt ccgacacaga cccaagagaa
     3481 ctcgaagcat tcgctgaaag attcaaacaa agaagaatca aactcggtgt tactcaagca
     3541 gatgtgggaa gcgcactggc taacctcaag atccctggag tcggcagcct gagccagtcc
     3601 accatctgtc gctttgaatc tctgaccctg tcccacaaca acatgattgc cctcaagcct
     3661 atcctccagg cttggctgga ggaggcagag ggagcacaac gcgagaagat gaacaagcca
     3721 gagctgttca acggaggcga gaagaagcgc aagcgcactt ccattgctgc acctgagaag
     3781 agatccctgg aggcatactt cgcagtgcag cccaggccat cttctgagaa gatcgcagct
     3841 attgccgaga agctcgacct caagaagaac gtggtcaggg tgtggttctg caatcagagg
     3901 cagaaacaga agagaatgaa gttctccgca acctactaat gtacaatcga tcgcccaccc
     3961 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4021 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4081 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     4141 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     4201 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     4261 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     4321 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     4381 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggacg gccgcacatg
     4441 taaggaaaat tttagggatg ttaaagaaaa aaataacaca aaacaaaata taaaaaaaat
     4501 ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg acagcagggg gctgtttcat
     4561 atactgatga cctctttata gccaaccttt gttcatggca gccagcatat gggcatatgt
     4621 tgccaaactc taaaccaaat actcattctg atgttttaaa tgatttgccc tcccatatgt
     4681 ccttccgagt gagagacaca aaaaattcca acacactatt gcaatgaaaa taaatttcct
     4741 ttattagcca gaagtcagat gctcaagggg cttcatgatg tccccataat ttttggcaga
     4801 gggaaaaaga tctcagtggt atttgtgagc cagggcattg gccacaccag tcaccacctt
     4861 ctgataggca gcctgcacct gaggagtgaa ttcacgcgtt ttagcctggc agcatatcga
     4921 gatcgaagtc atcgagagcg tcagcaggga gcatatccag atcaaaatca tccagggcat
     4981 cggcgggcag catgtccagg tcgaagtcgt ccagggcgtc ggtgggtccg ccgctctcgc
     5041 acttcagctg cttctcgagg ccgcagatga tcagttccag gccgaacagg aaggcgggct
     5101 cggcgccctg tctgtcgaac agctcgatgg cctgcttcag cagggggggc atgctgtcgg
     5161 tggtgggtgt ctctctttcc tctttggcga cctggtgttc ctgttcttcc agcacgcagc
     5221 ccagggtgaa gtggcccacg gcgctcaggg cgtacagggc gttttccagg ctgaagccct
     5281 gctggcacag gaaggccagc tggttttcca gtgtctcgta ctgcttctcg gtgggtctgg
     5341 tgcccaggtg cactttggcg ccgtcccggt ggctcagcag ggcgcatctg tagctcttgg
     5401 cgttgttccg caggaagtcc tgccagctct cgccttccag agggcagctg tgggtgtggt
     5461 gccggtccag catctcgatg ggcagggcgt ccagcagggc ccgcttgttc ttcacgtgcc
     5521 agtacagggt gggctgttcc acgcccagtt tctgggccag cttccgggtg gtcaggccct
     5581 cgatgcccac gccgttcagc agttccaggg cgctgttgat cactttgctc ttgtccagcc
     5641 ggctcatgtc gacattcttt gccaaaatga tgagacagca caataaccag cacgttgccc
     5701 aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct agagccgccg
     5761 gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc cccgaaggca gccgtccccc
     5821 cgcggacagc cccgaggctg gagagggaga aggggacggc ggcgcggcga cgcacgaagg
     5881 ccctccccgc ccatttcctt cctgccggcg ccgcaccgct tcgccccgcg cccgctagag
     5941 ggggtgcggc ggcgcctccc agatttcggc tccgcacaga tttgggacaa aggaagtccc
     6001 tgcgccctct cgcacgatta ccataaaagg caatggctgc ggctcgccgc gcctcgacag
     6061 ccgccggcgc tccgggggcc gccgcgcccc tcccccgagc cctccccggc ccgaggcggc
     6121 cccgccccgc ccggcacccc cacctgccgc caccccccgc ccggcacggc gagccccgcg
     6181 ccacgccccg tacggagccc cgcacccgaa gccgggccgt gctcagcaac tcggggaggg
     6241 gggtgcaggg ggggttgcag cccgaccgac gcgcccacac cccctgctca cccccccacg
     6301 cacacacccc gcacgcagcc tttgttcccc tcgcagcccc ccccgcaccg cggggcaccg
     6361 cccccggccg cgctcccctc gcgcacactg cggagcgcac aaagccccgc gccgcgcccg
     6421 cagcgctcac agccgccggg cagcgcggag ccgcacgcgg cgctccccac gcacacacac
     6481 acgcacgcac cccccgagcc gctccccccg cacaaagggc cctcccggag cccctcaagg
     6541 ctttcacgca gccacagaaa agaaacaagc cgtcattaaa ccaagcgcta attacagccc
     6601 ggaggagaag ggccgtcccg cccgctcacc tgtgggagta acgcggtcag tcagagccgg
     6661 ggcgggcggc gcgaggcggc ggcggagcgg ggcacggggc gaaggcagcg tcgcagcgac
     6721 tccccgcccg ccgcgcgctt cgctttttat agggccgccg ccgccgccgc ctcgccataa
     6781 aaggaaactt tcggagcgcg ccgctctgat tggctgccgc cgcacctctc cgcctcgccc
     6841 cgccccgccc ctcgccccgc cccgccccgc ctggcgcgcg cccccccccc ccccccgccc
     6901 ccatcgctgc acaaaataat taaaaaataa ataaatacaa aattgggggt ggggaggggg
     6961 gggagatggg gagagtgaag cagaacgtgg ggctcacctc gaccatggta atagcgatga
     7021 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
     7081 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
     7141 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
     7201 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
     7261 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
     7321 atgggctatg aactaatgac cccgtaattg attactatta ataactagtc aataatcaat
     7381 gtcgagaccc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc
     7441 ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg
     7501 cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa
     7561 ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc
     7621 tgatcagcgg gtttataact tcgtataatg tatgctatac gaagttatgg taccttactc
     7681 gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgaatttaaa
     7741 ttcaggcgcc aggctttctg gtcatgcacc aggttctagg gccctcaggc acttccacgt
     7801 cggcggtcac ggtgaagccc agtctctcgt agaagggcag gtttctgggg gcgcttgttt
     7861 ccaggaaggc gggcacgcca gccctttcag cagcttccac gccaggcagc accacagcag
     7921 atcccagtcc cttgccctgg tggtcaggtg acacgcccac ggtggccaga aaccaggcag
     7981 gctctttagg tctgtggggg gccagcaggc cttccatctg ctgctgggca gccagtctag
     8041 agccgctcag ctcggccatt ctaggtccga tctcggcgaa cacagcgccg gcttccacag
     8101 actcaggggt tgtccacaca gccacagcgg cgccatcatc ggccacccac actttgccga
     8161 tgtccaggcc cactctggtc agaaacagtt cctgcagctc ggtcactctc tcgatgtgtc
     8221 tgtcggggtc cacggtgtgt ctggtggcag ggtaatcggc gaaggcagcg gccagtgtcc
     8281 gcacagctct aggcacatcg tccctggtgg ccagccgcac tgtgggcttg tactcggtca
     8341 tagggccggg attctcctcc acgtcaccgc atgttagaag acttcctctg ccctcgccag
     8401 agcctctgct gtgcatcttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
     8461 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
     8521 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
     8581 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
     8641 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt
     8701 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct
     8761 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc
     8821 cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga
     8881 agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca
     8941 gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca
     9001 gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc
     9061 cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc
     9121 ccttgctcac catggtggcg ctagcgtagg cgccggtcac agcttggatc tgtaacggcg
     9181 cagaacagaa aacgaaacaa agacgtagag ttgagcaagc agggtcaggc aaagcgtgga
     9241 gagccggctg agtctaggta ggctccaagg gagcgccgga caaaggcccg gtctcgacct
     9301 gagctttaaa cttacctaga cggcggacgc agttcaggag gcaccacagg cgggaggcgg
     9361 cagaacgcga ctcaaccggc gtggatggcg gcctcaggta gggcggcggg cgcgtgaagg
     9421 agagatgcga gcccctcgaa gcttcagctg tgttctggcg gcaaacccgt tgcgaaaaag
     9481 aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg
     9541 gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccggt
     9601 tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgccca
     9661 ctgacgggca ccggagcgat cgcagatcga gagagaaatg ttctggcacc tgcacttgca
     9721 ctggggacag cctattttgc tagtttgttt tgtttcgttt tgttttgatg gagagcgtat
     9781 gttagtacta tcgattcaca caaaaaacca acacacagat gtaatgaaaa taaagatatt
     9841 ttatttcacc gcttgaaggc gatgtatgcc accagtccga cgacgacggc tgccaggatg
     9901 gagcaataca cgggaatcag gttatctgtg gtgccccggg tgaccactgg ctgggagctc
     9961 cccatgacag ttgtgaccac gccagccacg gtggaggcga tcagatcctg ctctggtggt
    10021 gcttctggct cctgtgtaga tggtgcggtg ctgtctgatc cttcaggggg ggtggaccga
    10081 gtaatccatc gccctgggat ttcctcgcac tcggcgtctg cccatctggt acattcgcgc
    10141 agctgccttt cggtatcctc gcacacagta caaggcaggc aggggtccac gtgattggcc
    10201 tcatcgctat atgtcccgtc aggacattcc tcgcacacgg tgttctgttt atcctggcag
    10261 ctaaagacca gccctgagcc tgcctcacac accctgcaag cttcacatct gccggtagtc
    10321 tcgtcctgat agtatccgta tgcacaccgg cagactgcat cgtcagcctc cacacaagga
    10381 gcactcatag actgcaggcc cacgcactca gtacatggct tgcaaggttc tgtggcactg
    10441 accacatcag agaatgtgac ggagtccaga caaggctcgc acacagtctg attggctccg
    10501 catggctgtg ccactccctc tcccaggtta caagctttac agcattctcc agaatgagtg
    10561 tacagccctg tggggcaggc ttccttcgcg atcgctccac ttcctggctc tcgctgtccc
    10621 tgaggatggt gttccagtct agcccgcagc tgttccagct ctcctgccag tccctcgacc
    10681 acgtgtcctc ctcgccatcc tgtggtcttt tcgtccatcc ctgacccctc gagctgcacc
    10741 tgtgaatgca ctccagttgc ggttgcgacc agaaacagaa tgatacatga ccagcccata
    10801 gggccgggat tctcctccac gtcaccgcat gttagaagac ttcctctgcc ctctccgctg
    10861 ccagatctct cgaggccctg tgggaggaag agaagaggtc agataacttc gtataatgta
    10921 tgctatacga agttatgcgg ccgctgggag aagggtaaga gagctcaatc ctcatcttac
    10981 atagaaggaa gtcagtagat aatatcttac atttaaaagt caagaaataa cagtctgacc
    11041 atactatcta gaaatatgga agttaacagt aaggaagtta aaacagttga aagtagtttc
    11101 gtctagaaag aaagacttgg ggaagaacaa agaaggctaa ttttcattat gagctttatt
    11161 gtaacagaaa agagaccact aactcagcta ggttttccca attcctcaac actttggggc
    11221 acagtgttta gtcagagctg tgatcactct ctgagcacat ggattgtatc ttaattttca
    11281 tctcattgaa aatatgtacc atttgtccaa gtggaaactg ggctgaagca tgactgtgac
    11341 caccaccaaa gaaatggcta gactcgctaa ggtctgctgg ggtgacccgt gattgtgttg
    11401 tgcttgatct tgttgagcat ggctctttta agtcaccata cctgcccctg ccaatgcacc
    11461 aaacaccaac ttaaagggac tttcagcttt tgtaagtcag tctcagcctt acttctgaac
    11521 catttctttg acttctttca tgactctgat cctgtcccag ttttaagcct cccagccacc
    11581 agtcatgctg ttttgctctg ctatttacat tcttctctac ctactttgga cttgatggta
    11641 tgcctactat attagttatt tattgctgtg taacaaacca ctctgaaaca tagtggctta
    11701 caaccacaac cattttattt tattatgaaa ctgtgggtca gaatgtatat tggcccattg
    11761 gcatgattct cctgctattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
    11821 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
    11881 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
    11941 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
    12001 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
    12061 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
    12121 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
    12181 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
    12241 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
    12301 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
    12361 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    12421 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    12481 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    12541 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    12601 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    12661 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    12721 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    12781 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    12841 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    12901 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    12961 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    13021 ccgcctacat acctcgctct gctaatcc
//
