LOCUS       sequence-321938-        9524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 9524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GPR144-Tango
FEATURES             Location/Qualifiers
     source          1..9524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3848..4462,4463..4849)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3915..3934)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4881..4898)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4887..5111
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5157..5585
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5244..5263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5454..5475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5594..5614)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5599..5928
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5779..5914
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5841..5860
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5995..6789
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6049..6068)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6659..6678
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6963..7084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7000..7019)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7054..7073
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7133..7149)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7133..7149)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7146..7168)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7157..7173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7181..7187,7188..7205,7206..7211))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7226..7247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7364..7381)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7535..8123)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7615..7634)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8294..9085,9086..9154))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8917..8936
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9155..9259)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9334..9353)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttctgcagat atccagcaca gtggcggccg cgccaccatg aagacgatca tcgccctgag
      781 ctacatcttc tgcctggtat tcgccgacta caaggacgat gatgacgcca gcatcgatat
      841 ggatgcccca tggggcgccg gagagcgctg gttgcatgga gccgcagtag atagatctgg
      901 ggtaagcctt ggtccacccc ctacgcctca agttaatcag ggtactctgg ggccacaggt
      961 ggctcccgta gcggcaggtg aagttgtcaa aacagccgga ggggtgtgca agttttccgg
     1021 ccagcgcctc agctggtggc aggcccaaga gagctgtgag cagcaattcg gccaccttgc
     1081 attgcagcca cctgacgggg ttctggcctc acggctgagg gacccggttt gggtgggcca
     1141 gagagaggca cctctccgcc ggcctccaca gcgcagggct agaacgactg cagtactggt
     1201 gtttgatgag cgaactgcag accgagctgc aagactgagg agcccactgc cagagctggc
     1261 cgccctgacg gcctgtaccc atgtgcagtg ggactgtgca tctcccgatc ctgcagccct
     1321 gttttccgtc gccgccccag cgcttcctaa cgctctccaa ctccgcgctt ttgctgagcc
     1381 cgggggcgtg gtaagagctg ccttggtggt gaggggacag catgctccct ttctggctgc
     1441 attccgggca gatggaaggt ggcatcacgt gtgcgcaaca tgggaacagc gaggcggtcg
     1501 gtgggcactg ttttcagatg gtcgccgcag ggcaggcgcc agaggtttgg gggccgggca
     1561 tccagtacca agcggcggta tcctggtgtt gggacaggat caggactccc ttggaggggg
     1621 gttttcagta agacacgccc ttagcggaaa tctcactgac ttccatttgt gggcaagggc
     1681 acttagccca gctcagttgc atagggcgcg cgcttgcgcg ccaccttctg agggacttct
     1741 gtttaggtgg gatcccggag ccctcgatgt gacaccctcc cttctgccta cagtgtgggt
     1801 caggttgttg tgccctgtgc caagcgagga atgcccgacc tggaaccctg gaccgcgctc
     1861 cgaaggcagc gaattgtgcc tggaacccca gccatttttg tgctgctata ggaccgagcc
     1921 ttaccgccga ctccaggatg cccaaagctg gcccggtcag gatgtcatca gtcgagtgaa
     1981 tgctctcgcg aatgacatcg tgctgctgcc ggatcccttg tccgaggtcc atggagctct
     2041 tagtcctgct gaggcctcat cattcctggg actgctcgaa cacgtcctgg ccatggagat
     2101 ggcacctctc gggccggcag ccctcctcgc tgttgtacgc tttctcaagc gcgttgtggc
     2161 gctgggtgcc ggtgacccag agctcctcct cacggggcca tgggagcagc tttcccaggg
     2221 cgttgtgagc gtggcctccc tggttttgga agaacaggtc gctgatactt ggctgtccct
     2281 ccgcgaagtt atcggaggcc caatggccct cgtcgcaagc gttcaacgac tggctcctct
     2341 cttgtcaacc tccatgacca gcgagagacc acgaatgagg attcagcacc ggcatgctgg
     2401 cctgtctgga gtcactgtga ttcatagctg gttcaccagc cgagtttttc agcacacgct
     2461 ggagggcccg gatctggagc cccaggcccc agctagctca gaagaagcca acagggtgca
     2521 gcgctttctt tctacccagg tgggttcagc aatcatcagt tctgaagtct gggacgtgac
     2581 aggagaagtt aatgtcgcta tgacattcca ccttcaacat agagcacagt ctcctctctt
     2641 tccgccacat ccacccagcc cgtacacagg gggcgcatgg gctactactg gctgcagcgt
     2701 cgccgcactg taccttgact ccaccgcctg tttctgtaat cattccacgt cattcgcaat
     2761 actgcttcag atatacgaag tacagcgagg acctgaggaa gagtctcttc tgcgaactct
     2821 gtctttcgtt ggctgtggcg tgagcttttg cgcactgaca accaccttcc tgctcttctt
     2881 ggtcgcagga gtgcccaaga gcgagcgcac taccgtacat aaaaacctga cattctcctt
     2941 ggcctccgcc gaaggctttc ttatgacatc cgagtgggcg aaagccaacg aggtcgcgtg
     3001 tgtggctgtg accgttgcaa tgcacttcct gtttttggtt gcctttagtt ggatgctggt
     3061 ggaaggtctc cttctctggc gcaaggtggt ggcagtcagc atgcaccctg ggcctggaat
     3121 gcggttgtac catgcaactg gctggggcgt tccagtcggg atcgtggcgg tgaccctcgc
     3181 aatgcttcca cacgattatg ttgcaccggg gcactgctgg ctgaacgtgc atacaaacgc
     3241 aatctgggca tttgtggggc ctgtcctctt tgtcctgact gcgaatactt gcattctggc
     3301 tcgcgttgtc atgattacag tgagcagcgc caggcggagg gccaggatgc tgagtccaca
     3361 gccttgtctc cagcagcaga tctggaccca gatctgggca actgttaaac ccgtgctggt
     3421 tctgctgcct gtgcttggat tgacctggct ggcaggcatt cttgtccacc tctcacccgc
     3481 atgggcctac gcggcagtcg gacttaattc cattcaaggg ttgtatatct ttctcgtgta
     3541 tgctgcctgc aacgaagagg tgcgatccgc actgcagcgg atggccgaga agaaggtagc
     3601 cgaagtactg cgggcattgg gggtttgggg aggggccgcc aaggagcact ccctcccgtt
     3661 ttccgtcctg ccactgtttc tgcctcccaa accctccaca ccgagacacc cactgaaagc
     3721 acctgcaatc gataccggtg gacgcacccc acccagcctg ggtccccaag atgagtcctg
     3781 caccaccgcc agctcctccc tggccaagga cacttcatcg accggtgaga acctgtactt
     3841 ccagctaaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
     3901 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
     3961 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
     4021 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
     4081 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
     4141 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
     4201 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
     4261 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
     4321 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
     4381 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
     4441 acttaaatgt gaaagtgggt ccgcgtacag ccgcgcgcgt acgaaaaaca attacgggtc
     4501 taccatcgag ggcctgctcg atctcccgga cgacgacgcc cccgaagagg cggggctggc
     4561 ggctccgcgc ctgtcctttc tccccgcggg acacacgcgc agactgtcga cggccccccc
     4621 gaccgatgtc agcctggggg acgagctcca cttagacggc gaggacgtgg cgatggcgca
     4681 tgccgacgcg ctagacgatt tcgatctgga catgttgggg gacggggatt ccccgggtcc
     4741 gggatttacc ccccacgact ccgcccccta cggcgctctg gatatggccg acttcgagtt
     4801 tgagcagatg tttaccgatg cccttggaat tgacgagtac ggtgggtagt ctagagggcc
     4861 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     4921 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4981 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5041 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     5101 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     5161 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5221 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5281 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5341 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5401 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5461 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5521 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5581 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5641 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5701 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5761 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5821 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     5881 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     5941 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     6001 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     6061 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     6121 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     6181 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     6241 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     6301 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     6361 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     6421 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     6481 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     6541 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     6601 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     6661 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     6721 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     6781 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     6841 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     6901 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     6961 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7021 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7081 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7141 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7201 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7261 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7321 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7381 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7441 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7501 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7561 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7621 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7681 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7741 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7801 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7861 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7921 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7981 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8041 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8101 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8161 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8221 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8281 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8341 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8401 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8461 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8521 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8581 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8641 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8701 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8761 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8821 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8881 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8941 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9001 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9061 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9121 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9181 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9241 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     9301 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     9361 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     9421 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     9481 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
