LOCUS       sequence-321939-       14115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium
            smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. 
            marinum and recX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14115)
  AUTHORS   Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC
  TITLE     A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient 
            Genome Editing in Mycobacterium tuberculosis.
  JOURNAL   mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 
            10.1128/mBio.02364-19.
  PUBMED    31992616
REFERENCE   2  (bases 1 to 14115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2020-01-28"; volume: "11(1). pii: mBio.02364-19. doi"; pages:
            " 10.1128/mBio.02364-19"
COMMENT     Sequence Label: pNHEJ-Cas9-recX
FEATURES             Location/Qualifiers
     source          1..14115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    31..49
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      3529..3556
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      4343..4931
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4832..4851
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9198..9209
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12844..12863)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             12855..13670
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(12928..12947)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tccctatcag tgatagagat aatctgggag tccctatcag tgatagagaa ggcggtatcg
       61 atatgtcgga cctggtgctg ggcctggata tcggcatcgg ctcggtgggc gtgggcatcc
      121 tgaacaaggt gaccggcgag atcatccaca agaactcgcg catcttcccg gccgcccagg
      181 ccgagaacaa cctggtgcgc cgcaccaacc gccagggccg ccgcctggcc cgccgcaaga
      241 agcaccgccg cgtgcgcctg aaccgcctgt tcgaggagtc gggcctgatc accgacttca
      301 ccaagatctc gatcaacctg aacccgtacc agctgcgcgt gaagggcctg accgacgagc
      361 tgtcgaacga ggagctgttc atcgccctga agaacatggt gaagcaccgc ggcatctcgt
      421 acctggacga cgcctcggac gacggcaact cgtcggtggg cgactacgcc cagatcgtga
      481 aggagaactc gaagcagctg gagaccaaga ccccgggcca gatccagctg gagcgctacc
      541 agacctacgg ccagctgcgc ggcgacttca ccgtggagaa ggacggcaag aagcaccgcc
      601 tgatcaacgt gttcccgacc tcggcctacc gctcggaggc cctgcgcatc ctgcagaccc
      661 agcaggagtt caacccgcag atcaccgacg agttcatcaa ccgctacctg gagatcctga
      721 ccggcaagcg caagtactac cacggcccgg gcaacgagaa gtcgcgcacc gactacggcc
      781 gctaccgcac ctcgggcgag accctggaca acatcttcgg catcctgatc ggcaagtgca
      841 ccttctaccc ggacgagttc cgcgccgcca aggcctcgta caccgcccag gagttcaacc
      901 tgctgaacga cctgaacaac ctgaccgtgc cgaccgagac caagaagctg tcgaaggagc
      961 agaagaacca gatcatcaac tacgtgaaga acgagaaggc catgggcccg gccaagctgt
     1021 tcaagtacat cgccaagctg ctgtcgtgcg acgtggccga catcaagggc taccgcatcg
     1081 acaagtcggg caaggccgag atccacacct tcgaggccta ccgcaagatg aagaccctgg
     1141 agaccctgga catcgagcag atggaccgcg agaccctgga caagctggcc tacgtgctga
     1201 ccctgaacac cgagcgcgag ggcatccagg aggccctgga gcacgagttc gccgacggct
     1261 cgttctcgca gaagcaggtg gacgagctgg tgcagttccg caaggccaac tcgtcgatct
     1321 tcggcaaggg ctggcacaac ttctcggtga agctgatgat ggagctgatc ccggagctgt
     1381 acgagacctc ggaggagcag atgaccatcc tgacccgcct gggcaagcag aagaccacct
     1441 cgtcgtcgaa caagaccaag tacatcgacg agaagctgct gaccgaggag atctacaacc
     1501 cggtggtggc caagtcggtg cgccaggcca tcaagatcgt gaacgccgcc atcaaggagt
     1561 acggcgactt cgacaacatc gtgatcgaga tggcccgcga gaccaacgag gacgacgaga
     1621 agaaggccat ccagaagatc cagaaggcca acaaggacga gaaggacgcc gccatgctga
     1681 aggccgccaa ccagtacaac ggcaaggccg agctgccgca ctcggtgttc cacggccaca
     1741 agcagctggc caccaagatc cgcctgtggc accagcaggg cgagcgctgc ctgtacaccg
     1801 gcaagaccat ctcgatccac gacctgatca acaactcgaa ccagttcgag gtggaccaca
     1861 tcctgccgct gtcgatcacc ttcgacgact cgctggccaa caaggtgctg gtgtacgcca
     1921 ccgccaacca ggagaagggc cagcgcaccc cgtaccaggc cctggactcg atggacgacg
     1981 cctggtcgtt ccgcgagctg aaggccttcg tgcgcgagtc gaagaccctg tcgaacaaga
     2041 agaaggagta cctgctgacc gaggaggaca tctcgaagtt cgacgtgcgc aagaagttca
     2101 tcgagcgcaa cctggtggac acccgctacg cctcgcgcgt ggtgctgaac gccctgcagg
     2161 agcacttccg cgcccacaag atcgacacca aggtgtcggt ggtgcgcggc cagttcacct
     2221 cgcagctgcg ccgccactgg ggcatcgaga agacccgcga cacctaccac caccacgccg
     2281 tggacgccct gatcatcgcc gcctcgtcgc agctgaacct gtggaagaag cagaagaaca
     2341 ccctggtgtc gtactcggag gaccagctgc tggacatcga gaccggcgag ctgatctcgg
     2401 acgacgagta caaggagtcg gtgttcaagg ccccgtacca gcacttcgtg gacaccctga
     2461 agtcgaagga gttcgaggac tcgatcctgt tctcgtacca ggtggactcg aagttcaacc
     2521 gcaagatctc ggacgccacc atctacgcca cccgccaggc caaggtgggc aaggacaagg
     2581 ccgacgagac ctacgtgctg ggcaagatca aggacatcta cacccaggac ggctacgacg
     2641 ccttcatgaa gatctacaag aaggacaagt cgaagttcct gatgtaccgc cacgacccgc
     2701 agaccttcga gaaggtgatc gagccgatcc tggagaacta cccgaacaag cagatcaacg
     2761 acaagggcaa ggaggtgccg tgcaacccgt tcctgaagta caaggaggag cacggctaca
     2821 tccgcaagta ctcgaagaag ggcaacggcc cggagatcaa gtcgctgaag tactacgact
     2881 cgaagctggg caaccacatc gacatcaccc cgaaggactc gaacaacaag gtggtgctgc
     2941 agtcggtgtc gccgtggcgc gccgacgtgt acttcaacaa gaccaccggc aagtacgaga
     3001 tcctgggcct gaagtacgcc gacctgcagt tcgacaaggg caccggcacc tacaagatct
     3061 cgcaggagaa gtacaacgac atcaagaaga aggagggcgt ggactcggac tcggagttca
     3121 agttcaccct gtacaagaac gacctgctgc tggtgaagga caccgagacc aaggagcagc
     3181 agctgttccg cttcctgtcg cgcaccatgc cgaagcagaa gcactacgtg gagctgaagc
     3241 cgtacgacaa gcagaagttc gagggcggcg aggccctgat caaggtgctg ggcaacgtgg
     3301 ccaactcggg ccagtgcaag aagggcctgg gcaagtcgaa catctcgatc tacaaggtgc
     3361 gcaccgacgt gctgggcaac cagcacatca tcaagaacga gggcgacaag ccgaagctgg
     3421 acttctaaat actctggcca tttttcttta ccgcctttga gtgagctgat accgctcgcc
     3481 gcagccgaac gaccgagcgc aacgcgtgcg gccgctgatt agctaagcag aaggccatcc
     3541 tgacggatgg cctttttcta gaaaaaaaaa aagcgccgca actgcggcgc tttttttttt
     3601 ttaattaagt gatagattgc gatgcgtgtg ctcggttgat ataatctgtc agatcaggag
     3661 gacttcgcat gtcgcggttg gataagtcca aggtcatcaa ctcggcgctg gagctgctga
     3721 acgaggtcgg tatcgaggga ctgaccacgc gcaagttggc ccaaaagctc ggcgtggaac
     3781 aaccgaccct ctactggcac gtgaagaaca agcgcgcgct gctggatgcg ttggccatcg
     3841 agatgctgga tcgccatcac acgcactttt gccccctgga gggcgagagc tggcaggatt
     3901 ttctgcggaa caacgcgaag tcgttccggt gcgccctcct gagccatcgg gatggcgcca
     3961 aagtccactt gggcacccgc ccgaccgaga agcaatacga gaccctggag aaccagctgg
     4021 ccttcctctg tcaacagggt ttttcgttgg agaacgccct gtatgccctg tccgccgtgg
     4081 gacacttcac cttgggctgc gtgttggagg atcaggaaca tcaggtggcg aaagaggaac
     4141 gggagacccc cacgacggat tcgatgcccc cgttgttgcg ccaggcgatc gaactgtttg
     4201 accaccaggg cgccgaaccc gcctttttgt tcgggctgga gctgatcatc tgcgggctgg
     4261 aaaagcaact gaagtgcgaa agcggttcgt gataactagt tccatgagcg tcagaccccg
     4321 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4381 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4441 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     4501 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4561 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4621 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4681 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4741 aaagcgccac gcttcccgag gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4801 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4861 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4921 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4981 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5041 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgagcccacc
     5101 agctccgtaa gttcgggcgc tgtgtggctc gtacccgcgc attcaggcgg cagggggtct
     5161 aacgggtcta aggcggcgtg tacggccgcc acagcggctc tcagcggccc ggaaacgtcc
     5221 tcgaaacgac gcatgtgttc ctcctggttg gtacaggtgg ttgggggtgc tcggctgtcg
     5281 ctggtgttcc accaccaggg ctcgacggga gagcggggga gtgtgcagtt gtggggtggc
     5341 ccctcagcga aatatctgac ttggagctcg tgtcggacca tacaccggtg attaatcgtg
     5401 gtctactacc aagcgtgagc cacgtcgccg acgaatttga gcagctctgg ctgccgtact
     5461 ggccgctggc aagcgacgat ctgctcgagg ggatctaccg ccaaagccgc gcgtcggccc
     5521 taggccgccg gtacatcgag gcgaacccaa cagcgctggc aaacctgctg gtcgtggacg
     5581 tagaccatcc agacgcagcg ctccgagcgc tcagcgcccg ggggtcccat ccgctgccca
     5641 acgcgatcgt gggcaatcgc gccaacggcc acgcacacgc agtgtgggca ctcaacgccc
     5701 ctgttccacg caccgaatac gcgcggcgta agccgctcgc atacatggcg gcgtgcgccg
     5761 aaggccttcg gcgcgccgtc gatggcgacc gcagttactc aggcctcatg accaaaaacc
     5821 ccggccacat cgcctgggaa acggaatggc tccactcaga tctctacaca ctcagccaca
     5881 tcgaggccga gctcggcgcg aacatgccac cgccgcgctg gcgtcagcag accacgtaca
     5941 aagcggctcc gacgccgcta gggcggaatt gcgcactgtt cgattccgtc aggttgtggg
     6001 cctatcgtcc cgccctcatg cggatctacc tgccgacccg gaacgtggac ggactcggcc
     6061 gcgcgatcta tgccgagtgc cacgcgcgaa acgccgaatt tccgtgcaac gacgtgtgtc
     6121 ccggaccgct accggacagc gaggtccgcg ccatcgccaa cagcatttgg cgttggatca
     6181 caaccaagtc gcgcatttgg gcggacggga tcgtggtcta cgaggccaca ctcagtgcgc
     6241 gccagtcggc catctcgcgg aagggcgcag cagcgcgcac ggcggcgagc acagttgcgc
     6301 ggcgcgcaaa gtccgcgtca gccatggagg cattgctatg agcgacggct acagcgacgg
     6361 ctacaaccgg cagccgactg tccgcaaaaa gcggcgcgtg accgccgccg aaggcgctcg
     6421 aatcaccgga ctatccgaac gccacgtcgt ccggctcgtg gcgcaggaac gcagcgagtg
     6481 gctcgccgag caggctgcac gccgcgaacg catccgcgcc tatcacgacg acgagggcca
     6541 ctcttggccg caaacggcca aacatttcgg gctgcatctg gacaccgtta agcgactcgg
     6601 ctatcgggcg aggaaagagc gtgcggcaga acaggaagcg gctcaaaagg cccacaacga
     6661 agccgacaat ccaccgctgt tctaacgcaa ttggggagcg ggtgtcgcgg gggttccgtg
     6721 gggggttccg ttgcaacggg tcggacaggt aaaagtcctg gtagacgcta gttttctggt
     6781 ttgggccatg cctgtctcgt tgcgtgtttc gttgcgcccg ttttgaatac cagccagacg
     6841 agacggggtt ctacgaatct tggtcgatac caagccattt ccgctgaata tcggggagct
     6901 caccgccaga atcggtggtt gtggtgatgt acgtggcgaa ctccgttgta gtgcctgtgg
     6961 tggcatccgt ggcgcggccg cggtacgcag tcgatcgtac gctagttaac tacgtcgaca
     7021 gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc ggcacgatcc gcgacgtgat
     7081 gatcggccgc atccccacgg tgctgcgcag tgagctctac gccatcccgg cgttgatctg
     7141 tgcgttcgca cgcacaggcc cggtgtgaga agggtctctg acgagcggga gaacccaccc
     7201 ggggtgggcg agtttgtcct gcgtgtgctc ggtcgagtag gctctgggag tacccgtgtg
     7261 tacgaccagc acggcataca tcatttcgac gccgagagat tcgccgcccg aaatgagcac
     7321 gatccgcatg cttataacag aaaggaggtt aataatgacg tcctcccggc cccggttgac
     7381 ttctgagccg tcggacgtcc ccgacggcga gcaggcgcag gatcctcgca cccgtgagga
     7441 gcaggccaag aacgtctgcc tgcgcctgct caccgtgcgg gcacgtaccc gggccgagct
     7501 cgaaacccag ctgaccaagc gcggctatcc cgacgacgtc agcgccaggg ttctcgaccg
     7561 gctcaccgag gtcggcctga tcgacgatga ggacttcgcc gagcagtggg tgcgttcacg
     7621 ccacctcaac gccggaaaag gcaagcgcgc cttggctgtc gagctccgta agaagggcgt
     7681 ggacgacgag gtgatctcgt cggctctggc cgatctcgac ccggccgccg agcgtcaacg
     7741 ggccgagcag ttggtgcgcg acaagttgcg ccgcgaacgt ctcgacggca caccggaaaa
     7801 cgacgtcaag gtcacccgca ggctcgtggg catgctggcg cgccgcggat acaaccagtc
     7861 gatggcctac gacgtggtca gcgtcgagtt ggccaacgag cgggagcgcc ggcgggtcta
     7921 gtaacgccga aaaccccgct tcggcggggt tttgccgcgg tctgcgttgt cgggaagatt
     7981 taaaataaaa aaggggacct ctagggtccc caaaagcttg gcgtgaattc ataacgtacc
     8041 caagaagcac agtgagtcgc ggatcgatgg cgggcgcggt atcggcgccg ccaacgtcac
     8101 ctcgtcgaga gccaccacct cactcggtga gcgcaagacg ttttcaccgg cttcgcgtaa
     8161 tccgtcggcg ccgcgcttga tcaaatcgag cagggcgact cccggcgcca tggcgtagat
     8221 cgaaccgtcg gacaacaccc cgacacgctc accgtccgca cttcgatacg tcacccattt
     8281 cattcgcgca ccacctcact cgggtttttg tccgggcgca acccacgcca gctggattgc
     8341 cgcaatcggt catcgggggt ccactcgcta tagcgcacct ccccgaccag cgtcggcgcg
     8401 acgaacgtaa cacccttggc ctcgcgcgcc ggaagcgcag cgtcgaatgg ggattcgtcg
     8461 gtatgcagcg gcgccagcgt cttcttgagg ttggccaggt cacgttcggt gaagccggtt
     8521 ccaacacggc cggcgaagtg caaaccgctc gggccgggaa tgcccatgag caacgatcca
     8581 atcccgctgc tgcgcccacc ctccccaact cgccaaccgc cgatgacgac ttcctgggtg
     8641 ttccagtgtt tgtccttgat ccacgccgac gagcgccgcc cgggttgata gccggagtcg
     8701 cggcgcttgg caatgactcc ctcccaaccg cgcttgtgcg agtaggccag cgcctcggcg
     8761 ccgtcaccgg gcaacaactc cggaacgatc agattgcccg cactgcccag ggtttccagt
     8821 agcttgcgcc ggtcgcggta tctggcccgc agtagcgagc gaccgtccag aaagagcaag
     8881 tcgaacgccc agaactcgat gcgggtggcg cgcacccggt tctgcatctc gttgaagctg
     8941 ggcactccgg aggagtcgag ggccaccaat tccccgtcga gcaccacgtg gtgatcagcg
     9001 agatcggcgg ccaatgaacg taattggggg tattccgcgg tgacgtcacg tccgctgcgg
     9061 gagcgcagtc gcagcgcgcc atggtcggct tcgaccagca gccggtagcc gtcccacttg
     9121 ccttcgaacg cccactgacc cgactcgaga cctgccaccg aaccgtggga ggtgagcatg
     9181 ggcgcgaggg tgtcgaaatc gaaggccgtc tggtccttca tgcgatgcgc cagccattgg
     9241 tccccgttgg tctgaatcag cgcgtaccgc cccgagattc gactgccgtg caggttgaca
     9301 atcacctccc ccttttccga accgtttctc gagtcgtcgc ggaacttctc ggtgtcatag
     9361 gtgccggaat cccagatgat caccttgccg gcgccgtact cccctttcgg gatgttgccc
     9421 tcgaacgtcg cgtattccag cggatgatct tcggtgtgca cggccaggtg gttcaccgac
     9481 gtggtttccg gcaggttttt cggcaccgcc catgacacca gtaccccatc acgctccagc
     9541 ctgaagtcgt aatgcagacg gcgcgcgtga tgctcttgga tcacgaaggt attaccctgc
     9601 ccgacaacgg gtctggctcg gggcaccggc tccggcgttt tcgacgcgtt gcgcatgctg
     9661 cgatatttga gcagccggtc gtcgccgggg aggtccgcgt ccagcgccgc aatcagatcg
     9721 ccatcgcggg tgaggcgggc aagcacctcg tcgtaggtca gctggcgcag ccccgggtcg
     9781 tcgagctcct cccagctgcg tggtgcggcg acggtcggtt gttcgcgccc acgcagcgaa
     9841 tacggcgcga tggtggtctt cgaaccgctg ttttggctcc agtccaggaa cactttcccg
     9901 gcccgcagac tcttggtcat ggtcgcggtg accaacgccg gcatcaccgt ttccagttgc
     9961 tgcgcgaccc gtttggccaa caccgtggcg cccttgctgc tcaccggctc gtcgagcggg
    10021 gtgtagaggt gcaggccctt gctaccgctg gtgagcggga gggtactcag gccgatgtcg
    10081 gcgagcagat cgcgcaccgc ccgcgccacc tgcgcgagtt gggccatcgt cacgccctcg
    10141 cccgggtcca ggtcgaacac caatcgggtg gccggcccgg gcgtgaggat ttcgcctttc
    10201 gacgtccact cggcaacgaa ccgccactgc gggacgtgca cctcgagggc ggcctgttgc
    10261 gcgatccagg ccagcccggt ggcgctgtcg atgatcggat aggtcgtcgt tcccgaccgg
    10321 tgcaccacgc tggcacgcgg cagccagtcc ggcgccgacg acgccagctg cttttcgaaa
    10381 aaggacgcct gatcgacgcc gttgggccag cgtttgcgag tcgccgggcg cccggcgata
    10441 tgcgggatca tcacctcggc gatgccggtg tagtaaccga agacgtcaga cttggtggtt
    10501 ccggtagccg ggtacagcac cttgtccgcg ttggtcagtg tcacctgcgg accattcgcc
    10561 tgtccaggag gccaggcctg cgccgaaccc atatggccaa cgtaacctgt cgccatggca
    10621 cgtgggctgg tgatcggtga agccctgatc gacatcgtcg atgggccgga cccggccgag
    10681 tatgtcgggg gcagccccct caacgtcgcg gtcggactgg ccagattggg ccgcgacgtc
    10741 gatctgctga cccacatcgg ccgcgacgcc cgcggacgac ggatcgccga gtacattgaa
    10801 tcctcaggtg tacaacttgt ttcgggaagc cagacggctg atcggacgcc caccgcgacg
    10861 gccaggatcg gccccgacgg ctcggccacc tacgcgttcg acctggaatg gcagataccc
    10921 gacacacccc ccgtggcgcc gccgcttctc gtgcacaccg gttccatcgc cgccgcgcgg
    10981 gaaccaggat gcttggcggt cgcggcgctg ctcgacgcct atcgggccgc ggccacggtg
    11041 agcttcgatc ccaatgtgcg tccgtcgctg agcgccgacc cggacctgac tcgggaacgc
    11101 atccaacgcc tcgtcgagcg cagcgacatc atcaaggcca gtgccgaaga cttgcactgg
    11161 atcgacccca cccagcctcc ggagcagacc gcgcgggcct ggctggcctg cggcccggcg
    11221 attgtcgcgc tgacgttggg cgatcagggc gcagtggcgt tctgcgcggc cggcccggca
    11281 agtgtgcccg cccagccggt tcgcgtggtc gataccgtcg gcgccggaga cgcgttcatg
    11341 gccggtctgc tcgacacgct gtgggagcag ggattgttag gtgccgaccg gcgcaccgag
    11401 ttgcgcaaga tcggcgtgtc ggcgctgacc tctgcactcg aagtcgcggc cctgacctcg
    11461 gcgttgaccg ttgcccgtgc cggcgccgat ctgccctatc gggccgacct gcggcagtcg
    11521 cgatgaccac cgcacgcccg caatcgggcc cggcgagggt gcacgaaatg ccgcgtgatg
    11581 cggcgtgtcg ccgtgccgac acgcacgctc gcgagctgac cggggctggg gcaggcagca
    11641 ggactccggt gccgcggcgc actgttaacc gacccgggtt ctgggcatac tgactccatg
    11701 cgctccatct ggaagggttc aatcgctttc gggctggtga atgtgccggt caaggtgtac
    11761 agcgccactc aggaccacga catcaagttc catcaggtgc acgccaagga caacggacgc
    11821 atccggtaca agcgggtatg cgaggtctgt ggcgaggtgg tcgaatactc cgacctggcc
    11881 cgcgccttcg aatccgacga tggccagatg gtgatcatca acgacgacga cctcgccacg
    11941 ttgcccgaag atcgcagccg cgaaatcgag gtgctggaat tcgtcccagc cgaagaagtc
    12001 gatccgatga tgattgaccg cagctacttc ctggagcccg actcgaagtc ctctaaatcg
    12061 tatgtgctgc tggccaagac gctcgccgag acggagcgga tggcgatcgt gcatttcacg
    12121 ctgcgcagca agacgcggct ggcggcgttg cgggtcaagg acttcggcaa gcgcgaggtc
    12181 atggtggtgc ataccctgtt gtggccagac gagatccgcg acccggattt cccggtgctg
    12241 gacaaggagg tggagatcaa acccgcggag ctcaagatgg ccggccaagt ggtggagtcg
    12301 atggcagacg acttcaaccc ggaccgctac cgcgacacct atcaggagca actgcaggag
    12361 ctgatcgacg ccaaactcga aggcggggaa gcatttaccg ccgaagaaca acccgcagag
    12421 ctggacgaga ccgaagatgt ctccgacctg ctggccaagc tggaggcgag cgtgaaagcg
    12481 cgttcgggcg acgggaagtc agcggcgaag aagagcaccg ccgaaaaggc gccggccaag
    12541 aaggcccggg ccaaacaagc tgccaagtcc tgaccagcgc ccgacttgtt cacatatgct
    12601 cgtcgtgccg ccgagaggag tttttgcgtg aagtccaacc gatctggcgt cgtgctcagc
    12661 ctgctgctcg ccagcacatt ggcgttgtcg gcatgcggag gccattccag caatacctcg
    12721 tccacctgac gtcggggggg ggggaaagcc acgttgtgtc tcaaaatctc tgatgttaca
    12781 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
    12841 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa
    12901 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
    12961 caggtgcgac aatctatcgc ttgtatggga agccccatgc gccagagttg tttctgaaac
    13021 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
    13081 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
    13141 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt
    13201 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
    13261 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
    13321 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
    13381 aacaagtctg gaaagaaatg cataatcttt tgccattctc accggattca gtcgtcactc
    13441 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
    13501 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
    13561 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
    13621 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
    13681 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg
    13741 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga
    13801 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct
    13861 caccaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga
    13921 gtcagcaaca ccttcttcac gaggcagacc tcactagtgg tctgacgctc atggaacaat
    13981 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttat ctgttgtttg aaaaaaaaaa
    14041 gccccgcgat tgcggggctt ttttttttca tatgtctgac cagggaaaat agccctctga
    14101 cctggggatt tgact
//
