LOCUS       sequence-321940-        9254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GPR113-Tango
FEATURES             Location/Qualifiers
     source          1..9254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3578..4192,4193..4579)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3645..3664)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4611..4628)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4617..4841
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4887..5315
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4974..4993)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5184..5205
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5324..5344)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5329..5658
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5509..5644
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5571..5590
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5725..6519
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5779..5798)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6389..6408
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6693..6814
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6730..6749)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6784..6803
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6863..6879)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6863..6879)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6876..6898)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6887..6903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6911..6917,6918..6935,6936..6941))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6956..6977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7094..7111)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7265..7853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7345..7364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8024..8815,8816..8884))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8647..8666
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8885..8989)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9064..9083)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttctgcagat atccagcaca gtggcggccg cgccaccatg aagacgatca tcgccctgag
      781 ctacatcttc tgcctggtat tcgccgacta caaggacgat gatgacgcca gcatcgatat
      841 gaccacccga aagctcagcg cccattccgc tgcaacacct ggctacaagg cggtgaccca
      901 caagcaccat acaggatggg cgcggatggc aaaaacgggc cttccagaga aaggacagtc
      961 ccaggcaggt ggagagtccg ggtccggaca acttctcgac caagagaatg gggcaggtga
     1021 gtccgccctg gtttctgttt acgtccacct cgattttccc gacaaaacat ggcctcccga
     1081 actttcaaga accttgacac tgccagccgc cagtgccagc agtagccccc gccccctgct
     1141 gactgggctc cgcctcacga ctggcgaata catgtcatgc tttgaagccc agggatttaa
     1201 gtggaatctc tacgaagttg tgagagtgcc tctgaaggcc acagacgtcg cgaggcttcc
     1261 ataccaactg agcatttctt gtgccacctc tcccggtttc cagctttcct gttgcatccc
     1321 tagcacgaac ctcgcgtata ccgctgcctg gtcacccgga gagggcagta aggcaagctc
     1381 attcaatgaa agcggaagcc agtgctttgt actcgccgtg cagcgctgcc caatggctga
     1441 cacgacatat gcctgcgacc tgcagtccct gggccttgcc ccactcaggg tgcccatcag
     1501 cattaccatc attcaagatg gcgacattac atgtccagaa gatgcgtccg tgctgacctg
     1561 gaacgtaacc aaagcagggc atgtggccca agcaccctgc ccggagagca aacgcggcat
     1621 tgtgcggagg ctctgtggag ctgatggcgt gtggggcccc gttcactcta gttgtaccga
     1681 tgctcgactc ctggcgctgt tcactcgaac aaaactgctg caggccgggc aggggtcacc
     1741 tgcagaagaa gtgccgcaga ttctggctca gctgcccggc caagccgcag aggctagttc
     1801 accttccgat ttgctgaccc ttctgtctac catgaaatat gtcgcaaaag tggtagccga
     1861 agccagaata cagcttgacc ggagagctct gaaaaatctg ctgatcgcca ccgacaaagt
     1921 gctggacatg gacactcgct ctctctggac tttggcacag gctcgcaaac cttgggcagg
     1981 ctcaaccctc cttcttgccg tagagacact ggcttgtagc ctttgcccgc aggatcaccc
     2041 ctttgcattc tctttgccaa acgtgctgct tcagtcccag ctgtttggac ctaccttccc
     2101 cgccgattac tccatctcat tccccacccg gccgcctttg caagcccaga ttccaaggca
     2161 ctccctggca ccgcttgtca gaaatggaac cgaaatctct attacatctc tcgttctgag
     2221 aaagttggat catttgctcc catccaacta cggccaagga ctgggtgact ctctgtatgc
     2281 cacccccggt ctggtgctgg taatatctat catggctggg gacagggcat tcagtcaggg
     2341 cgaggtcatt atggacttcg gcaataccga cgggagtccc cactgtgtct tttgggacca
     2401 ctcactgttc cagggacgcg gtggatggag caaagagggg tgtcaagccc aggtggccag
     2461 cgccagcccc acagcccagt gcctgtgtca gcacctgacc gcttttagcg tgctgatgtc
     2521 cccccacacg gtaccagaag agccagcgct cgcccttctg acccaggttg gtcttggagc
     2581 cagtattctc gctctgctgg tttgccttgg ggtgtattgg ctggtctggc gggtagtcgt
     2641 gcggaacaag atcagctact tcagacacgc tgccctgctg aacatggtgt tctgcctcct
     2701 ggctgccgat acctgctttc tgggtgcgcc cttcctcagc ccaggccctc ggagccctct
     2761 gtgcctggca gcggcgtttc tctgccattt cctgtatctg gctacatttt tttggatgct
     2821 ggcccaagct cttgtacttg cccatcaatt gctttttgtc tttcatcagc tcgcgaaaca
     2881 tcgggtcctg ccccttatgg tcctgctggg ctatctctgt ccactcggtc tggccggcgt
     2941 cacactcgga ctgtatctgc ctcaaggaca gtaccttcgg gagggcgagt gttggctcga
     3001 cggaaaggga ggggccttgt acactttcgt aggacccgtc ctggcaatca ttggcgtcaa
     3061 tggcttggtt ctggcgatgg cgatgcttaa gctcctgagg ccttctctgt ccgagggccc
     3121 gcccgcagaa aagagacaag ctcttctcgg tgtaatcaag gctctgctca ttctcacgcc
     3181 tatcttcggg cttacctggg ggcttggact cgcaacactg ctggaggagg ttagcacagt
     3241 tccacactac atctttacca ttttgaacac cctgcagggc gtgtttatcc ttcttttcgg
     3301 gtgcttgatg gaccgcaaaa ttcaggaagc tttgaggaaa agattttgca gagcccaggc
     3361 acccagctcc accatcagct tggccactaa cgagggctgc attctggagc attctaaggg
     3421 aggctccgat actgccagga agaccgacgc tagcgagatc gataccggtg gacgcacccc
     3481 acccagcctg ggtccccaag atgagtcctg caccaccgcc agctcctccc tggccaagga
     3541 cacttcatcg accggtgaga acctgtactt ccagctaaga ttagataaaa gtaaagtgat
     3601 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     3661 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     3721 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     3781 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
     3841 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
     3901 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
     3961 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     4021 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     4081 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     4141 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag
     4201 ccgcgcgcgt acgaaaaaca attacgggtc taccatcgag ggcctgctcg atctcccgga
     4261 cgacgacgcc cccgaagagg cggggctggc ggctccgcgc ctgtcctttc tccccgcggg
     4321 acacacgcgc agactgtcga cggccccccc gaccgatgtc agcctggggg acgagctcca
     4381 cttagacggc gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga
     4441 catgttgggg gacggggatt ccccgggtcc gggatttacc ccccacgact ccgcccccta
     4501 cggcgctctg gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat
     4561 tgacgagtac ggtgggtagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     4621 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     4681 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     4741 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     4801 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     4861 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     4921 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4981 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5041 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5101 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5161 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5221 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5281 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     5341 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5401 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5461 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5521 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5581 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5641 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     5701 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     5761 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5821 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5881 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     5941 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6001 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6061 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6121 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6181 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6241 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6301 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6361 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6421 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6481 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     6541 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     6601 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6661 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     6721 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6781 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     6841 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6901 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6961 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7021 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7081 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7141 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7201 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7261 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7381 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7441 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7501 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7561 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7621 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7681 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7741 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7801 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7861 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7921 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7981 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8041 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8101 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8161 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8221 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8281 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8341 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8401 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8461 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8521 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8581 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8641 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8701 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8761 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8821 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8881 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8941 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9001 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     9061 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     9121 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     9181 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     9241 cagatatacg cgtt
//
