LOCUS       sequence-321945-        9617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express SV-Tag construct in a Cre-independent manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini 
            BL
  TITLE     Rapid purification and metabolomic profiling of synaptic vesicles 
            from mammalian brain.
  JOURNAL   Elife. 2020 Oct 12;9. pii: 59699. doi: 10.7554/eLife.59699.
  PUBMED    33043885
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-12"; volume: "9. pii: 59699. doi"; pages: " 
            10.7554/eLife.59699"
COMMENT     Sequence Label: pAAV hSynapsin SV-tag
FEATURES             Location/Qualifiers
     source          1..9617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    310..426
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(693..737)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    922..1155
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1648..1773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1820..2000
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2001..2227)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2255..2275)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2255..2273)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2294..2310)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2294..2310)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2307..2329)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2318..2334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2342..2348,2349..2366,2367..2372))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2387..2408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2696..3284)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2776..2795)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3455..4246,4247..4315))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4078..4097
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4316..4420)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4446..4901)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4583..4604)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4795..4814
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   4973..5102
                     /label=AAV2 ITR
     promoter        5184..5631
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      5640..5649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5779..5797)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6047..6066
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6241..6264
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             7080..7133
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8094..8120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8133..8159
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8172..8198
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8211..8237
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8250..8276
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8289..8315
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8328..8354
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8367..8393
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8406..8432
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    8448..9036
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8501..8521)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8919..8930)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9039..9055)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9040..9056)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    9085..9206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9096..9115)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9150..9169
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   9368..9497
                     /label=AAV2 ITR
ORIGIN
        1 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
       61 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
      121 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
      181 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
      241 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactcccaag
      301 cttatcgata aaattttgaa ttttgtaatt tgtttttgta attctttagt ttgtatgtct
      361 gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt
      421 ttaaaagtta accgataccg tcgagatccg ttcactaatc gaatggatct gtctctgtct
      481 ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg
      541 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
      601 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
      661 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
      721 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
      781 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
      841 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
      901 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
      961 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
     1021 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
     1081 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
     1141 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     1201 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     1261 ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg cttgctgtgc
     1321 ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
     1381 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
     1441 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
     1501 tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac
     1561 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
     1621 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
     1681 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
     1741 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
     1801 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
     1861 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     1921 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     1981 tggtctaacc agagagaccc gtttatgtat cgagctaggc acttaaatac aatatctctg
     2041 caatgcggca attcagtggt tcgtccaatc catgtcagac ccgtctgttg ccttcctaat
     2101 aaggcacgat cgtaccacct tacttccacc aatcggcatg cacggtgctt tttctctcct
     2161 tgtaaggcat gttgctaact catcgttacc atgttgcaag actacaagag tattgcataa
     2221 gactacatta agcttgcagc tccagctttt gttcccttta gtgagggtta attgcgcgct
     2281 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     2341 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     2401 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2461 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2521 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2581 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2641 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2701 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2761 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2821 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2881 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2941 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3001 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3061 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3121 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3181 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3241 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3301 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3361 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3421 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3481 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3541 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3601 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3661 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3721 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3781 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3841 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3901 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3961 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4021 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4081 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4141 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4201 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4261 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4321 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4381 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4441 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     4501 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     4561 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     4621 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     4681 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     4741 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     4801 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     4861 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     4921 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctgcgcgc
     4981 tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc
     5041 ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc
     5101 cttgtagtta atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat
     5161 ctctgcagag ggccctgcgt atgagtgcaa gtgggtttta ggaccaggat gaggcggggt
     5221 gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac ccccattccc
     5281 caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc
     5341 gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac
     5401 cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgca aactcccctt
     5461 cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg
     5521 cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg
     5581 cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca gtcgaattcg
     5641 ccaccatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca
     5701 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     5761 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     5821 gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg
     5881 acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     5941 acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc
     6001 tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga
     6061 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     6121 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     6181 agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca
     6241 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg
     6301 agggccgcca ccacctgttc ctggggcatg gtaccggcag caccggcagc ggcagctccg
     6361 gcaccgcctc ctccgaggac aacaacatgg ccgtgattaa ggagtttatg aggtttaaag
     6421 tcaggatgga gggcagcatg aacgggcacg agtttgagat tgaaggagag ggggaaggga
     6481 ggccctatga gggcacccag acagcaaagc tgaaagtgac aaagggcggc cctctgccat
     6541 tcgcatggga catcctgtct cctcagttta tgtatggaag taaagcctac gtgaaacacc
     6601 ctgctgacat tccagattac aagaaactga gcttcccaga ggggtttaag tgggaaagag
     6661 tgatgaactt cgaagacggg ggcctggtga ctgtcaccca ggacagctct ctgcaggacg
     6721 gcactctgat ctataaggtg aaaatgcgcg gcaccaattt tccccctgat ggcccagtca
     6781 tgcagaagaa gacaatgggc tgggaggctt caacagaaag gctgtacccc agagacggcg
     6841 tgctgaaggg agagatccac caggcactga agctgaaaga tggagggcat tatctggtcg
     6901 agttcaaaac catctacatg gccaagaaac ccgtgcagct gcctggctac tattacgtcg
     6961 acacaaagct ggatattact agccataacg aagactacac cattgtggag cagtatgagc
     7021 ggagcgaggg gaggcaccat ctgtttctgt acggcatgga cgagctgtac aagactagtg
     7081 agggcagagg aagtcttctg acatgcggag acgtggaaga gaatcccggc cctgccccag
     7141 gctcatctag aatggacgtg gtgaatcagc tggtggctgg gggtcagttc cgggtggtca
     7201 aggagcccct tggcttcgtg aaggtgctgc agtgggtctt tgccatcttc gcctttgcta
     7261 cgtgcggcag ctacaccgga gagcttcggc tgagcgtgga gtgtgccaac aagacggaga
     7321 gtgccctcaa catcgaagtc gaatttgagt acccattcag gctgcaccaa gtgtactttg
     7381 atgcaccctc ctgcgttaaa gggggcacta ccaagatctt cctagttggt gactactcct
     7441 cctcggctga gttctttgtc accgtggctg tgtttgcctt cctctactcc atgggggccc
     7501 tggccaccta catcttcctg cagaacaagt accgagagaa caacaaaggg ccaatgatgg
     7561 acttcctggc cacagcagtg ttcgctttca tgtggctagt tagctcatcc gcctgggcca
     7621 aaggcctgtc cgatgtgaag atggccactg acccagagaa cattatcaag gagatgccta
     7681 tgtgccgcca gacaggaaac acatgcaagg aactgaggga ccctgtgact tcaggactca
     7741 acacctcggt ggtgtttggc ttcctgaacc tggtgctctg ggttggcaac ctatggttcg
     7801 tgttcaagga gacaggctgg gccgccccat tcatgcgcgc acctccaggc gccccagaaa
     7861 agcaaccagc tcctggcgat gcctacggcg atgcgggcta tgggcagggc cccggaggct
     7921 atgggcccca ggactcctac gggcctcagg gtggttatca acccgattac gggcagccag
     7981 ccagcggcgg tggcggtggc tacgggcctc agggcgacta tgggcagcaa ggctacggcc
     8041 aacagggtgc gcccacctcc ttctccaatc agatgtcgcg aggagggagc ggctatccct
     8101 atgacgtgcc tgattacgcc ggcacaggat cctaccccta tgatgtgcct gactacgctg
     8161 gcagcgccgg atacccttat gatgtgcctg attatgctgg agggagcggc tacccatacg
     8221 atgttccaga ttacgctgga acaggcagct atccctatga cgtcccggac tatgcaggca
     8281 gcgccggata tccatatgac gttccagatt acgctggagg ctccggctac ccttatgatg
     8341 tgcccgatta tgccggaaca ggcagctatc cctatgacgt gcctgactat gctggatccg
     8401 ccggctaccc ttatgacgtg cccgattacg cctgaaagct tatcgataat caacctctgg
     8461 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     8521 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     8581 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     8641 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     8701 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     8761 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     8821 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     8881 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     8941 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     9001 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgacccg
     9061 ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     9121 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     9181 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     9241 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     9301 tcgataagga tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt
     9361 aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     9421 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     9481 agcgagcgag cgcgcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9541 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9601 ggcgagcggt atcagct
//
