LOCUS       sequence-321947-        5960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mouse SNX27 without the F3 domain cDNA C-terminally tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Halff EF, Szulc BR, Lesept F, Kittler JT
  TITLE     SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory 
            Signaling.
  JOURNAL   Cell Rep. 2019 Nov 26;29(9):2599-2607.e6. doi: 
            10.1016/j.celrep.2019.10.096.
  PUBMED    31775031
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.10"; journalName: "Cell Rep"; date: 
            "2019-11-26- 26"; volume: "29"; issue: "9"; pages: "2599-2607.e6"
COMMENT     Sequence Label: pEGFP-N1-mSNX27-Delta-F3
FEATURES             Location/Qualifiers
     source          1..5960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1958..1967
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1964..1966,1967..1969,1970..2683)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1964..1966,1967..1969,1970..2680)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2009..2030)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2270..2289)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2617..2638
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2806..2927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2843..2862)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2897..2916
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2934..3389)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3071..3092)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3283..3302
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3416..3520
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3522..3879
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3522..3713
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3545..3565)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3730..3865
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3914..4708
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3914..4708
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3968..3987)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4578..4597
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4896..4915)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4940..4987
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5316..5904
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5805..5824
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggactcagat ctcgagctca agcttatggc ggacgaggac ggggaaggta ttcacccctc
      721 agccccgcac cggaatggag gcggcggcgg ggggtctggg ctccactgcg ccgggaacgg
      781 tggcggggga ggcggcggcc cgcgggtcgt gcgcattgtc aagtccgaat ccggctacgg
      841 cttcaacgtg cggggccaag tgagcgaggg cgggcaactg cggagcatca acggggagct
      901 gtacgcgccg ctgcagcatg tgagcgccgt gctgcccggg ggggcggccg accgggccgg
      961 ggtgcgcaaa ggggaccgca tcctggaggt gaacggcgtg aatgttgagg gggcgacaca
     1021 caagcaggtg gtggacctga tccgagcagg cgagaaggaa ttgatcttga cagtgttgtc
     1081 tgtacctcct catgaggctg ataacctaga tcccagtgac gactccttgg gacaatcatt
     1141 ttatgattac acagaaaagc aagcagtgcc catatcggtc cccacataca aacatgtgga
     1201 gcagaatggt gagaagtttg tggtatacaa tgtttacatg gctgggaggc agctgtgttc
     1261 taagcggtac cgggagtttg ccatactgca ccaaaacctg aagagagaat ttgccaactt
     1321 tacatttcct cgacttccag gaaagtggcc attctcatta tcagaacaac aattggatgc
     1381 ccggcgtcgt gggttggaag aatatctaga aaaggtgtgt tcaatccgag tcattggtga
     1441 gagtgacatc atgcaggaat tcctgtctga gtctgatgag aattacaatg gtgtgtctga
     1501 tgtagaactg agagtagcac taccggatgg aacaacggtt actgtcagag ttaagaagaa
     1561 cagtactaca gaccaagtgt accaggctat tgcagcaaag gttggcatgg acagtacaac
     1621 agtgaattac tttgccttat ttgaagtgat caatcattcc tttgtacgta agctggcacc
     1681 taatgaattt cctcataaac tctacgtcca gaactataca tcagctgtgc caggcacttg
     1741 cctgaccatt cgcaagtggc tctttacaac agaagaagaa gtcctcttaa atgacaatga
     1801 ccttgctgtt acgtacttct ttcatcaggc tgttgatgat gtaaagaaag gttacattaa
     1861 agcagaagag aaatcttacc agttacagaa gctccatgaa caaaggaaga tggtcatgta
     1921 cctcaacatg ctaaggacct gcgagggccc accggtcgcc accatggtga gcaagggcga
     1981 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2041 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2101 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2161 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2221 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2281 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2341 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2401 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2461 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     2521 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     2581 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2641 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag
     2701 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     2761 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     2821 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     2881 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt
     2941 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     3001 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     3061 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     3121 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3181 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     3241 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3301 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     3361 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     3421 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3481 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     3541 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3601 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3661 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3721 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3781 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3841 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     3901 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3961 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4021 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4081 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4141 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4201 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4261 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4321 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4381 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4441 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4501 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4561 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4621 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4681 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     4741 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4801 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     4861 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     4921 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     4981 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     5041 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     5101 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     5161 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5221 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5281 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5341 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5401 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5461 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5521 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5581 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5641 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5701 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5761 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5821 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5881 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5941 ttttgctggc cttttgctca
//
