LOCUS       sequence-321949-       11528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium
            tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum 
            and recX..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC
  TITLE     A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient 
            Genome Editing in Mycobacterium tuberculosis.
  JOURNAL   mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 
            10.1128/mBio.02364-19.
  PUBMED    31992616
REFERENCE   2  (bases 1 to 11528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2020-01-28"; volume: "11(1). pii: mBio.02364-19. doi"; pages:
            " 10.1128/mBio.02364-19"
COMMENT     Sequence Label: pNHEJ-recX-sacB
FEATURES             Location/Qualifiers
     source          1..11528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..54)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             46..861
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(119..138)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        1149..1594
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(1595..1681,1682..3016)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFVKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     rep_origin      3104..3692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3593..3612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7917..7928
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
       61 cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga tttatatggg
      121 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
      181 aagccccatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
      241 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
      301 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca
      361 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
      421 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
      481 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
      541 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataatctt
      601 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
      661 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
      721 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
      781 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
      841 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
      901 acgctgactt gacgggacgg cggctttgtt gaataaatcg aacttttgct gagttgaagg
      961 atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa
     1021 tcaccaactg gtccacctac aacaaagctc tcaccaaccg tggctccctc actttctggc
     1081 tggatgatgg ggcgattcag gcctggtatg agtcagcaac accttcttca cgaggcagac
     1141 ctcaccatca catatacctg ccgttcacta ttatttagtg aaatgagata ttatgatatt
     1201 ttctgaattg tgattaaaaa ggcaacttta tgcccatgca acagaaacta taaaaaatac
     1261 agagaatgaa aagaaacaga tagatttttt agttctttag gcccgtagtc tgcaaatcct
     1321 tttatgattt tctatcaaac aaaagaggaa aatagaccag ttgcaatcca aacgagagtc
     1381 taatagaatg aggtcgaaaa gtaaatcgcg cgggtttgtt actgataaag caggcaagac
     1441 ctaaaatgtg taaagggcaa agtgtatact ttggcgtcac cccttacata ttttaggtct
     1501 ttttttattg tgcgtaacta acttgccatc ttcaaacagg agggctggaa gaagcagacc
     1561 gctaacacag tacataaaaa aggagacatg aacgatgaac atcaaaaagt ttgtaaaaca
     1621 agcaacagta ttaaccttta ctaccgcact gctggcagga ggcgcaactc aagcgtttgc
     1681 gaaagaaacg aaccaaaagc catataagga aacatacggc atttcccata ttacacgcca
     1741 tgatatgctg caaatccctg aacagcaaaa aaatgaaaaa tatcaagttc ctgagttcga
     1801 ttcgtccaca attaaaaata tctcttctgc aaaaggcctg gacgtttggg acagctggcc
     1861 attacaaaac gctgacggca ctgtcgcaaa ctatcacggc taccacatcg tctttgcatt
     1921 agccggagat cctaaaaatg cggatgacac atcgatttac atgttctatc aaaaagtcgg
     1981 cgaaacttct attgacagct ggaaaaacgc tggccgcgtc tttaaagaca gcgacaaatt
     2041 cgatgcaaat gattctatcc taaaagacca aacacaagaa tggtcaggtt cagccacatt
     2101 tacatctgac ggaaaaatcc gtttattcta cactgatttc tccggtaaac attacggcaa
     2161 acaaacactg acaactgcac aagttaacgt atcagcatca gacagctctt tgaacatcaa
     2221 cggtgtagag gattataaat caatctttga cggtgacgga aaaacgtatc aaaatgtaca
     2281 gcagttcatc gatgaaggca actacagctc aggcgacaac catacgctga gagatcctca
     2341 ctacgtagaa gataaaggcc acaaatactt agtatttgaa gcaaacactg gaactgaaga
     2401 tggctaccaa ggcgaagaat ctttatttaa caaagcatac tatggcaaaa gcacatcatt
     2461 cttccgtcaa gaaagtcaaa aacttctgca aagcgataaa aaacgcacgg ctgagttagc
     2521 aaacggcgct ctcggtatga ttgagctaaa cgatgattac acactgaaaa aagtgatgaa
     2581 accgctgatt gcatctaaca cagtaacaga tgaaattgaa cgcgcgaacg tctttaaaat
     2641 gaacggcaaa tggtacctgt tcactgactc ccgcggatca aaaatgacga ttgacggcat
     2701 tacgtctaac gatatttaca tgcttggtta tgtttctaat tctttaactg gcccatacaa
     2761 gccgctgaac aaaactggcc ttgtgttaaa aatggatctt gatcctaacg atgtaacctt
     2821 tacttactca cacttcgctg tacctcaagc gaaaggaaac aatgtcgtga ttacaagcta
     2881 tatgacaaac agaggattct acgcagacaa acaatcaacg tttgcgccaa gcttcctgct
     2941 gaacatcaaa ggcaagaaaa catctgttgt caaagacagc atccttgaac aaggacaatt
     3001 aacagttaac aaataaaaac gcaaaagaaa atgccgatat tgactaccgg aagcagtgtg
     3061 ttccatgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3121 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3181 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3241 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3301 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3361 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3421 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3481 acctacagcg tgagctatga gaaagcgcca cgcttcccga ggggagaaag gcggacaggt
     3541 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3601 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3661 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3721 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3781 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3841 agcgcaacgc gtgagcccac cagctccgta agttcgggcg ctgtgtggct cgtacccgcg
     3901 cattcaggcg gcagggggtc taacgggtct aaggcggcgt gtacggccgc cacagcggct
     3961 ctcagcggcc cggaaacgtc ctcgaaacga cgcatgtgtt cctcctggtt ggtacaggtg
     4021 gttgggggtg ctcggctgtc gctggtgttc caccaccagg gctcgacggg agagcggggg
     4081 agtgtgcagt tgtggggtgg cccctcagcg aaatatctga cttggagctc gtgtcggacc
     4141 atacaccggt gattaatcgt ggtctactac caagcgtgag ccacgtcgcc gacgaatttg
     4201 agcagctctg gctgccgtac tggccgctgg caagcgacga tctgctcgag gggatctacc
     4261 gccaaagccg cgcgtcggcc ctaggccgcc ggtacatcga ggcgaaccca acagcgctgg
     4321 caaacctgct ggtcgtggac gtagaccatc cagacgcagc gctccgagcg ctcagcgccc
     4381 gggggtccca tccgctgccc aacgcgatcg tgggcaatcg cgccaacggc cacgcacacg
     4441 cagtgtgggc actcaacgcc cctgttccac gcaccgaata cgcgcggcgt aagccgctcg
     4501 catacatggc ggcgtgcgcc gaaggccttc ggcgcgccgt cgatggcgac cgcagttact
     4561 caggcctcat gaccaaaaac cccggccaca tcgcctggga aacggaatgg ctccactcag
     4621 atctctacac actcagccac atcgaggccg agctcggcgc gaacatgcca ccgccgcgct
     4681 ggcgtcagca gaccacgtac aaagcggctc cgacgccgct agggcggaat tgcgcactgt
     4741 tcgattccgt caggttgtgg gcctatcgtc ccgccctcat gcggatctac ctgccgaccc
     4801 ggaacgtgga cggactcggc cgcgcgatct atgccgagtg ccacgcgcga aacgccgaat
     4861 ttccgtgcaa cgacgtgtgt cccggaccgc taccggacag cgaggtccgc gccatcgcca
     4921 acagcatttg gcgttggatc acaaccaagt cgcgcatttg ggcggacggg atcgtggtct
     4981 acgaggccac actcagtgcg cgccagtcgg ccatctcgcg gaagggcgca gcagcgcgca
     5041 cggcggcgag cacagttgcg cggcgcgcaa agtccgcgtc agccatggag gcattgctat
     5101 gagcgacggc tacagcgacg gctacaaccg gcagccgact gtccgcaaaa agcggcgcgt
     5161 gaccgccgcc gaaggcgctc gaatcaccgg actatccgaa cgccacgtcg tccggctcgt
     5221 ggcgcaggaa cgcagcgagt ggctcgccga gcaggctgca cgccgcgaac gcatccgcgc
     5281 ctatcacgac gacgagggcc actcttggcc gcaaacggcc aaacatttcg ggctgcatct
     5341 ggacaccgtt aagcgactcg gctatcgggc gaggaaagag cgtgcggcag aacaggaagc
     5401 ggctcaaaag gcccacaacg aagccgacaa tccaccgctg ttctaacgca attggggagc
     5461 gggtgtcgcg ggggttccgt ggggggttcc gttgcaacgg gtcggacagg taaaagtcct
     5521 ggtagacgct agttttctgg tttgggccat gcctgtctcg ttgcgtgttt cgttgcgccc
     5581 gttttgaata ccagccagac gagacggggt tctacgaatc ttggtcgata ccaagccatt
     5641 tccgctgaat atcggggagc tcaccgccag aatcggtggt tgtggtgatg tacgtggcga
     5701 actccgttgt agtgcctgtg gtggcatccg tggcgcggcc gcggtacgca gtcgatcgta
     5761 cgctagttaa ctacgtcgac agaaatattg gatcgtcggc accgtcacgg ccgtgggagg
     5821 cggcacgatc cgcgacgtga tgatcggccg catccccacg gtgctgcgca gtgagctcta
     5881 cgccatcccg gcgttgatct gtgcgttcgc acgcacaggc ccggtgtgag aagggtctct
     5941 gacgagcggg agaacccacc cggggtgggc gagtttgtcc tgcgtgtgct cggtcgagta
     6001 ggctctggga gtacccgtgt gtacgaccag cacggcatac atcatttcga cgccgagaga
     6061 ttcgccgccc gaaatgagca cgatccgcat gcttataaca gaaaggaggt taataatgac
     6121 ggtgtcctgc ccgcccccgt cgacttctga gcgcgaagag caggcgcggg cactgtgcct
     6181 gcgcctgctc accgcgcgat cccgcacccg cgccgagtta gccggccagc tggccaagcg
     6241 cggctacccc gaagacatcg gcaaccgggt attggatcgg ctggccgccg ttggcctggt
     6301 ggatgacacc gacttcgccg aacaatgggt tcagtccagg cgggcgaacg cagcaaagag
     6361 caagcgcgcg ttggctgccg agctgcacgc caagggcgtc gacgacgacg tgatcaccac
     6421 ggtgctcggg ggcatcgacg ccggtgccga acgggggcgg gcggaaaagc tggtacgggc
     6481 caggctgcgg cgggaggtgc tgatcgacga cggcaccgac gaagcgcggg tgagccgcag
     6541 gctggtggcg atgttggcgc gccgtgggta cggccagacc ttggcgtgcg aggtggttat
     6601 cgccgagctg gccgccgagc gggagcgccg acgcgtctaa taacgccgaa aaccccgctt
     6661 cggcggggtt ttgccgcggt ctgcgttgtc gggaagattt aaaataaaaa aggggacctc
     6721 tagggtcccc aaaagcttgg cgtgaattca taacgtaccc aagaagcaca gtgagtcgcg
     6781 gatcgatggc gggcgcggta tcggcgccgc caacgtcacc tcgtcgagag ccaccacctc
     6841 actcggtgag cgcaagacgt tttcaccggc ttcgcgtaat ccgtcggcgc cgcgcttgat
     6901 caaatcgagc agggcgactc ccggcgccat ggcgtagatc gaaccgtcgg acaacacccc
     6961 gacacgctca ccgtccgcac ttcgatacgt cacccatttc attcgcgcac cacctcactc
     7021 gggtttttgt ccgggcgcaa cccacgccag ctggattgcc gcaatcggtc atcgggggtc
     7081 cactcgctat agcgcacctc cccgaccagc gtcggcgcga cgaacgtaac acccttggcc
     7141 tcgcgcgccg gaagcgcagc gtcgaatggg gattcgtcgg tatgcagcgg cgccagcgtc
     7201 ttcttgaggt tggccaggtc acgttcggtg aagccggttc caacacggcc ggcgaagtgc
     7261 aaaccgctcg ggccgggaat gcccatgagc aacgatccaa tcccgctgct gcgcccaccc
     7321 tccccaactc gccaaccgcc gatgacgact tcctgggtgt tccagtgttt gtccttgatc
     7381 cacgccgacg agcgccgccc gggttgatag ccggagtcgc ggcgcttggc aatgactccc
     7441 tcccaaccgc gcttgtgcga gtaggccagc gcctcggcgc cgtcaccggg caacaactcc
     7501 ggaacgatca gattgcccgc actgcccagg gtttccagta gcttgcgccg gtcgcggtat
     7561 ctggcccgca gtagcgagcg accgtccaga aagagcaagt cgaacgccca gaactcgatg
     7621 cgggtggcgc gcacccggtt ctgcatctcg ttgaagctgg gcactccgga ggagtcgagg
     7681 gccaccaatt ccccgtcgag caccacgtgg tgatcagcga gatcggcggc caatgaacgt
     7741 aattgggggt attccgcggt gacgtcacgt ccgctgcggg agcgcagtcg cagcgcgcca
     7801 tggtcggctt cgaccagcag ccggtagccg tcccacttgc cttcgaacgc ccactgaccc
     7861 gactcgagac ctgccaccga accgtgggag gtgagcatgg gcgcgagggt gtcgaaatcg
     7921 aaggccgtct ggtccttcat gcgatgcgcc agccattggt ccccgttggt ctgaatcagc
     7981 gcgtaccgcc ccgagattcg actgccgtgc aggttgacaa tcacctcccc cttttccgaa
     8041 ccgtttctcg agtcgtcgcg gaacttctcg gtgtcatagg tgccggaatc ccagatgatc
     8101 accttgccgg cgccgtactc ccctttcggg atgttgccct cgaacgtcgc gtattccagc
     8161 ggatgatctt cggtgtgcac ggccaggtgg ttcaccgacg tggtttccgg caggtttttc
     8221 ggcaccgccc atgacaccag taccccatca cgctccagcc tgaagtcgta atgcagacgg
     8281 cgcgcgtgat gctcttggat cacgaaggta ttaccctgcc cgacaacggg tctggctcgg
     8341 ggcaccggct ccggcgtttt cgacgcgttg cgcatgctgc gatatttgag cagccggtcg
     8401 tcgccgggga ggtccgcgtc cagcgccgca atcagatcgc catcgcgggt gaggcgggca
     8461 agcacctcgt cgtaggtcag ctggcgcagc cccgggtcgt cgagctcctc ccagctgcgt
     8521 ggtgcggcga cggtcggttg ttcgcgccca cgcagcgaat acggcgcgat ggtggtcttc
     8581 gaaccgctgt tttggctcca gtccaggaac actttcccgg cccgcagact cttggtcatg
     8641 gtcgcggtga ccaacgccgg catcaccgtt tccagttgct gcgcgacccg tttggccaac
     8701 accgtggcgc ccttgctgct caccggctcg tcgagcgggg tgtagaggtg caggcccttg
     8761 ctaccgctgg tgagcgggag ggtactcagg ccgatgtcgg cgagcagatc gcgcaccgcc
     8821 cgcgccacct gcgcgagttg ggccatcgtc acgccctcgc ccgggtccag gtcgaacacc
     8881 aatcgggtgg ccggcccggg cgtgaggatt tcgcctttcg acgtccactc ggcaacgaac
     8941 cgccactgcg ggacgtgcac ctcgagggcg gcctgttgcg cgatccaggc cagcccggtg
     9001 gcgctgtcga tgatcggata ggtcgtcgtt cccgaccggt gcaccacgct ggcacgcggc
     9061 agccagtccg gcgccgacga cgccagctgc ttttcgaaaa aggacgcctg atcgacgccg
     9121 ttgggccagc gtttgcgagt cgccgggcgc ccggcgatat gcgggatcat cacctcggcg
     9181 atgccggtgt agtaaccgaa gacgtcagac ttggtggttc cggtagccgg gtacagcacc
     9241 ttgtccgcgt tggtcagtgt cacctgcgga ccattcgcct gtccaggagg ccaggcctgc
     9301 gccgaaccca tatggccaac gtaacctgtc gccatggcac gtgggctggt gatcggtgaa
     9361 gccctgatcg acatcgtcga tgggccggac ccggccgagt atgtcggggg cagccccctc
     9421 aacgtcgcgg tcggactggc cagattgggc cgcgacgtcg atctgctgac ccacatcggc
     9481 cgcgacgccc gcggacgacg gatcgccgag tacattgaat cctcaggtgt acaacttgtt
     9541 tcgggaagcc agacggctga tcggacgccc accgcgacgg ccaggatcgg ccccgacggc
     9601 tcggccacct acgcgttcga cctggaatgg cagatacccg acacaccccc cgtggcgccg
     9661 ccgcttctcg tgcacaccgg ttccatcgcc gccgcgcggg aaccaggatg cttggcggtc
     9721 gcggcgctgc tcgacgccta tcgggccgcg gccacggtga gcttcgatcc caatgtgcgt
     9781 ccgtcgctga gcgccgaccc ggacctgact cgggaacgca tccaacgcct cgtcgagcgc
     9841 agcgacatca tcaaggccag tgccgaagac ttgcactgga tcgaccccac ccagcctccg
     9901 gagcagaccg cgcgggcctg gctggcctgc ggcccggcga ttgtcgcgct gacgttgggc
     9961 gatcagggcg cagtggcgtt ctgcgcggcc ggcccggcaa gtgtgcccgc ccagccggtt
    10021 cgcgtggtcg ataccgtcgg cgccggagac gcgttcatgg ccggtctgct cgacacgctg
    10081 tgggagcagg gattgttagg tgccgaccgg cgcaccgagt tgcgcaagat cggcgtgtcg
    10141 gcgctgacct ctgcactcga agtcgcggcc ctgacctcgg cgttgaccgt tgcccgtgcc
    10201 ggcgccgatc tgccctatcg ggccgacctg cggcagtcgc gatgaccacc gcacgcccgc
    10261 aatcgggccc ggcgagggtg cacgaaatgc cgcgtgatgc ggcgtgtcgc cgtgccgaca
    10321 cgcacgctcg cgagctgacc ggggctgggg caggcagcag gactccggtg ccgcggcgca
    10381 ctgttaaccg acccgggttc tgggcatact gactccatgc gctccatctg gaagggttca
    10441 atcgctttcg ggctggtgaa tgtgccggtc aaggtgtaca gcgccactca ggaccacgac
    10501 atcaagttcc atcaggtgca cgccaaggac aacggacgca tccggtacaa gcgggtatgc
    10561 gaggtctgtg gcgaggtggt cgaatactcc gacctggccc gcgccttcga atccgacgat
    10621 ggccagatgg tgatcatcaa cgacgacgac ctcgccacgt tgcccgaaga tcgcagccgc
    10681 gaaatcgagg tgctggaatt cgtcccagcc gaagaagtcg atccgatgat gattgaccgc
    10741 agctacttcc tggagcccga ctcgaagtcc tctaaatcgt atgtgctgct ggccaagacg
    10801 ctcgccgaga cggagcggat ggcgatcgtg catttcacgc tgcgcagcaa gacgcggctg
    10861 gcggcgttgc gggtcaagga cttcggcaag cgcgaggtca tggtggtgca taccctgttg
    10921 tggccagacg agatccgcga cccggatttc ccggtgctgg acaaggaggt ggagatcaaa
    10981 cccgcggagc tcaagatggc cggccaagtg gtggagtcga tggcagacga cttcaacccg
    11041 gaccgctacc gcgacaccta tcaggagcaa ctgcaggagc tgatcgacgc caaactcgaa
    11101 ggcggggaag catttaccgc cgaagaacaa cccgcagagc tggacgagac cgaagatgtc
    11161 tccgacctgc tggccaagct ggaggcgagc gtgaaagcgc gttcgggcga cgggaagtca
    11221 gcggcgaaga agagcaccgc cgaaaaggcg ccggccaaga aggcccgggc caaacaagct
    11281 gccaagtcct gaccagcgcc cgacttgttc acatatgctc gtcgtgccgc cgagaggagt
    11341 ttttgcgtga agtccaaccg atctggcgtc gtgctcagcc tgctgctcgc cagcacattg
    11401 gcgttgtcgg catgcggagg ccattccagc aatacctcgt ccacctgacg tcgggggggg
    11461 gggaaagcca cgttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
    11521 catcatga
//
