LOCUS       sequence-321950-        6314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mouse SNX27 cDNA with a point mutation H112A C-terminally tagged 
            with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Halff EF, Szulc BR, Lesept F, Kittler JT
  TITLE     SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory 
            Signaling.
  JOURNAL   Cell Rep. 2019 Nov 26;29(9):2599-2607.e6. doi: 
            10.1016/j.celrep.2019.10.096.
  PUBMED    31775031
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.10"; journalName: "Cell Rep"; date: 
            "2019-11-26- 26"; volume: "29"; issue: "9"; pages: "2599-2607.e6"
COMMENT     Sequence Label: pEGFP-N1-mSNX27-H112A
FEATURES             Location/Qualifiers
     source          1..6314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..441
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        442..645
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     596..616
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2331..2340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2337..2339,2340..2342,2343..3056)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2337..2339,2340..2342,2343..3053)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2382..2403)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2643..2662)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2990..3011
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3179..3300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3216..3235)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3270..3289
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3307..3762)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3444..3465)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3656..3675
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3789..3893
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3895..4252
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3895..4086
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3918..3938)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4103..4238
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4287..5081
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4287..5081
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4341..4360)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4951..4970
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5269..5288)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5313..5360
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5689..6277
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6178..6197
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
       61 gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc
      121 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      181 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      241 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      301 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      361 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      421 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      481 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      541 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      601 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
      661 cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttatggcg gacgaggacg
      721 gggaaggtat tcacccctca gccccgcacc ggaatggagg cggcggcggg gggtctgggc
      781 tccactgcgc cgggaacggt ggcgggggag gcggcggccc gcgggtcgtg cgcattgtca
      841 agtccgaatc cggctacggc ttcaacgtgc ggggccaagt gagcgagggc gggcaactgc
      901 ggagcatcaa cggggagctg tacgcgccgc tgcagcatgt gagcgccgtg ctgcccgggg
      961 gggcggccga ccgggccggg gtgcgcaaag gggaccgcat cctggaggtg aacggcgtga
     1021 atgttgaggg ggcgacagcc aagcaggtgg tggacctgat ccgagcaggc gagaaggaat
     1081 tgatcttgac agtgttgtct gtacctcctc atgaggctga taacctagat cccagtgacg
     1141 actccttggg acaatcattt tatgattaca cagaaaagca agcagtgccc atatcggtcc
     1201 ccacatacaa acatgtggag cagaatggtg agaagtttgt ggtatacaat gtttacatgg
     1261 ctgggaggca gctgtgttct aagcggtacc gggagtttgc catactgcac caaaacctga
     1321 agagagaatt tgccaacttt acatttcctc gacttccagg aaagtggcca ttctcattat
     1381 cagaacaaca attggatgcc cggcgtcgtg ggttggaaga atatctagaa aaggtgtgtt
     1441 caatccgagt cattggtgag agtgacatca tgcaggaatt cctgtctgag tctgatgaga
     1501 attacaatgg tgtgtctgat gtagaactga gagtagcact accggatgga acaacggtta
     1561 ctgtcagagt taagaagaac agtactacag accaagtgta ccaggctatt gcagcaaagg
     1621 ttggcatgga cagtacaaca gtgaattact ttgccttatt tgaagtgatc aatcattcct
     1681 ttgtacgtaa gctggcacct aatgaatttc ctcataaact ctacgtccag aactatacat
     1741 cagctgtgcc aggcacttgc ctgaccattc gcaagtggct ctttacaaca gaagaagaag
     1801 tcctcttaaa tgacaatgac cttgctgtta cgtacttctt tcatcaggct gttgatgatg
     1861 taaagaaagg ttacattaaa gcagaagaga aatcttacca gttacagaag ctccatgaac
     1921 aaaggaagat ggtcatgtac ctcaacatgc taaggacctg cgagggctac aatgaaatca
     1981 tcttccctca ctgtgcctgt gactcgagga ggaagggaca tgttatcaca gccatcagca
     2041 tcacgcactt taaacttcat gcatgcactg aagaagggca gctggagaac caggtaatag
     2101 cgtttgaatg ggatgagatg cagcgatggg acacagatga agaaggaatg gccttctgtt
     2161 ttgaatatgc gcgaggagag aagaagcctc gatgggttaa aatcttcaca ccatatttca
     2221 attacatgca tgaatgcttt gagagggtgt tctgcgagct caagtggaga aaagagaata
     2281 ttttccagat ggcgaggtca cagcagaggg atgtggccac cccaccggtc gccaccatgg
     2341 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2401 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     2461 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     2521 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     2581 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     2641 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     2701 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     2761 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     2821 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     2881 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2941 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3001 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg
     3061 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     3121 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     3181 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3241 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3301 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     3361 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     3421 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     3481 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     3541 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3601 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3661 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3721 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     3781 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3841 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     3901 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3961 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4021 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4081 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4141 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4201 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     4261 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     4321 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     4381 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     4441 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     4501 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     4561 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     4621 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     4681 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     4741 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     4801 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     4861 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     4921 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     4981 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     5041 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     5101 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     5161 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     5221 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     5281 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     5341 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     5401 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     5461 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     5521 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5581 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5641 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5701 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5761 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5821 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5881 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5941 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6001 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6061 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6121 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6181 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6241 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6301 acggttcctg gcct
//
