LOCUS       sequence-321954-        6356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in 
            Sf9 insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Verardi R, Lindesmith LC, Tsybovsky Y, Gorman J, Chuang GY, Edwards 
            CE, Brewer-Jensen PD, Mallory ML, Ou L, Schon A, Shi W, Tully ES, 
            Georgiou G, Baric RS, Kwong PD
  TITLE     Disulfide stabilization of human norovirus GI.1 virus-like particles
            focuses immune response toward blockade epitopes.
  JOURNAL   NPJ Vaccines. 2020 Dec 14;5(1):110. doi: 10.1038/s41541-020-00260-w.
  PUBMED    33318483
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "NPJ 
            Vaccines."; date: "2020-12-14"; volume: "5(1):110. doi"; pages: " 
            10.1038/s41541-020-00260-w"
COMMENT     Sequence Label: pFastBac1_GI.1_G131C-N172C
FEATURES             Location/Qualifiers
     source          1..6356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    1943..2077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1980..1999)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2034..2053
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2106..2271
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2455..2909
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2542..2561)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2751..2772
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2935..3039
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3040..3108,3109..3900)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3258..3277)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4071..4659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4560..4579
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4813..4830
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4964..5188
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5255..5788)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5977..5982,5983..5999,6000..6005))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atccatgatg atggcttcaa aggacgctac ttcttctgtg gacggtgctt
      181 ctggagctgg tcagctggtc ccagaggtga acgcttccga cccactggcc atggaccctg
      241 tggctggctc ttcaaccgct gtggccactg ctggacaggt caaccccatc gacccatgga
      301 tcatcaacaa cttcgtgcag gcccctcagg gcgagttcac tatctcaccc aacaacaccc
      361 caggtgacgt cctgttcgac ctgtccctgg gccctcacct gaaccccttc ctgctgcacc
      421 tgagccagat gtacaacggc tgggtcggaa acatgagggt gagaatcatg ctggccggta
      481 acgctttcac cgccggcaag atcatcgtct catgcatccc tccctgcttc ggttcccaca
      541 acctgactat cgcccaggct accctgttcc cacacgtcat cgctgacgtg aggactctgg
      601 acccaatcga ggtgcctctg gaagacgtca gaaacgtgct gttccactgc aacgacagga
      661 accagcagac catgagactg gtctgcatgc tgtacacccc tctgaggact ggtggcggaa
      721 ctggagacag cttcgtggtc gccggtcgtg tgatgacttg cccatctcct gacttcaact
      781 tcctgttcct ggtcccacct accgtggagc agaagactag gccattcacc ctgcctaacc
      841 tgcccctgtc cagcctgtcc aacagcagag ctcccctgcc aatctcttca atgggcatca
      901 gccctgacaa cgtccagtct gtgcagttcc agaacggacg ctgcactctg gacggacgtc
      961 tggtcggtac cactcccgtg tctctgtcac acgtcgccaa gatcaggggc acttccaacg
     1021 gaaccgtgat caacctgact gagctggacg gtaccccatt ccaccctttc gaaggccctg
     1081 ctcccatcgg attccctgac ctgggtggct gcgactggca catcaacatg actcagttcg
     1141 gccactccag ccagacccag tacgacgtgg acaccactcc cgacaccttc gtcccacacc
     1201 tgggtagcat ccaggccaac ggtatcggct ctggaaacta cgtgggcgtc ctgtcttgga
     1261 tctcaccccc atcccaccca tccggaagcc aggtggacct gtggaagatc cctaactacg
     1321 gttcttcaat caccgaggct actcacctgg ccccctctgt ctaccctccc ggtttcggcg
     1381 aagtgctggt cttcttcatg agcaagatgc caggacctgg tgcttacaac ctgccctgcc
     1441 tgctgccaca ggagtacatc tcacacctcg cttctgagca ggctccaact gtgggtgaag
     1501 ctgccctgct gcactacgtc gacccagaca ccggtcgcaa cctgggcgag ttcaaggcct
     1561 accctgacgg attcctgact tgcgtgccca acggagcttc cagcggtcct cagcagctgc
     1621 ccatcaacgg agtgttcgtc ttcgtgagct gggtgtctcg tttctaccag ctgaagcctg
     1681 tcggtactgc ttcttcagct aggggtaggc tgggcctgcg ccgttgatga gaattcaaag
     1741 gcctacgtcg acgagctcac tagtcgcggc cgctttcgaa tctagagcct gcagtctcga
     1801 ggcatgcggt accaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt
     1861 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     1921 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     1981 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2041 gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctaggag
     2101 atccgaacca gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat
     2161 tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt
     2221 aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat
     2281 ttttcttcct gttatgtttt taatcaaaca tcctgccaac tccatgtgac aaaccgtcat
     2341 cttcggctac tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa
     2401 tgtttgtaat tgactgaata tcaacgctta tttgcagcct gaatggcgaa tgggacgcgc
     2461 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     2521 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     2581 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     2641 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     2701 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttcttaata gtggactctt
     2761 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2821 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2881 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     2941 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3001 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3061 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3121 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3181 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3241 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3301 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3361 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3421 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3481 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3541 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3601 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3661 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3721 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3781 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3841 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3901 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3961 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4021 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4081 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4141 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4201 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     4261 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4321 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4381 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4441 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4501 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4561 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4621 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4681 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4741 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4801 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     4861 ctccttacgc atctgtgcgg tatttcacac cgcatagacc agccgcgtaa cctggcaaaa
     4921 tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa
     4981 gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat
     5041 agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg
     5101 gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt
     5161 ggccaagggc atggtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg
     5221 cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat
     5281 caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt
     5341 caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga
     5401 ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat
     5461 catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag
     5521 cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca
     5581 agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact
     5641 cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac
     5701 atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct
     5761 gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     5821 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca
     5881 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     5941 gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat
     6001 gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc
     6061 agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc
     6121 acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat
     6181 ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg
     6241 accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc
     6301 caggactcta gctatagttc tagtggttgg ctacgtatac tccggaatat taatag
//
