LOCUS       sequence-321965-       10227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the constitutive expression of the PhiC31 integrase, 
            C-terminally fused with its reversion factor (RDF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 10227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (G...
FEATURES             Location/Qualifiers
     source          1..10227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1303..1932
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2361..3434
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3500..3694
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3930..3952)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4038..4178
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4093..4112
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4193..4210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4364..4952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4444..4463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5040..5855)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5040..5855)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5763..5782
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    6288..6312
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        6945..7289
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7233..7253
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             7363..9177
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             9931..9951
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    join(10205..10227,1..2)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
       61 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
      121 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
      181 catctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
      241 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
      301 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
      361 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
      421 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
      481 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
      541 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
      601 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
      661 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
      721 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
      781 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
      841 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
      901 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
      961 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
     1021 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
     1081 cgtgttcgag ccgcccgcgc acctctcaac cgtgcggctg catgaaatcc tggccggttt
     1141 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
     1201 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
     1261 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
     1321 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
     1381 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
     1441 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
     1501 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
     1561 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
     1621 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
     1681 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
     1741 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
     1801 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
     1861 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
     1921 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
     1981 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
     2041 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
     2101 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
     2161 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
     2221 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
     2281 ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc
     2341 cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg
     2401 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac
     2461 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca
     2521 aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg
     2581 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca
     2641 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
     2701 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca
     2761 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc
     2821 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg
     2881 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct
     2941 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
     3001 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg
     3061 aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca
     3121 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg
     3181 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat
     3241 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
     3301 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg
     3361 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat
     3421 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag
     3481 gtcgaaaagg actctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg
     3541 ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt
     3601 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta
     3661 ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag
     3721 agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc
     3781 ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag
     3841 ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc
     3901 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc
     3961 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     4021 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     4081 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     4141 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     4201 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4261 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4321 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4381 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4441 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4501 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4561 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4621 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4681 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4741 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4801 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4861 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4921 agcagattac gcgcagaaaa aaaggatctc cagaagatcc tttgatcttt tctacggggt
     4981 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtgat
     5041 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     5101 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     5161 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     5221 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     5281 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
     5341 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     5401 gcctgagcga gtcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     5461 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     5521 tcttctaata cctggaatgc tgttttccct gggatcgcag tggtgagtaa ccatgcatca
     5581 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     5641 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     5701 aactctggcg catcgggctt cccatacaat cggtagattg tcgcacctga ttgcccgaca
     5761 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     5821 cttgagcaag acgtttcccg ttgaatatgg ctcataacag aacttattat ttccttcctc
     5881 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
     5941 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
     6001 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc
     6061 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
     6121 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
     6181 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
     6241 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
     6301 tgtggtgtaa acataacgaa ttcgtctcag gagactagag ccaagctgat ctcctttgcc
     6361 ccggagatca ccatggacga ctttctctat ctctacgatc taggaagaaa gttcgacgga
     6421 gaaggtgacg ataccatgtt caccaccgat aatgagaaga ttagcctctt caatttcaga
     6481 aagaatgctg acccacagat ggttagagag gcctacgcgg caggtctgat caagacgatc
     6541 tacccgagta ataatctcca ggagatcaaa taccttccca agaaggttaa agatgcagtc
     6601 aaaagattca ggactaactg catcaagaac acagagaaag atatatttct caagatcaga
     6661 agtactattc cagtatggac gattcaaggc ttgcttcata aaccaaggca agtaatagag
     6721 attggagtct ctaagaaagt agttcctact gaatcaaagg ccatggagtc aaaaattcag
     6781 atcgaggatc taacagaact cgccgtgaag actggcgaac agttcataca gagtctttta
     6841 cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac tctcgtctac
     6901 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
     6961 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
     7021 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
     7081 atcgttcaag atgcccctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     7141 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     7201 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     7261 taaggaagtt catttcattt ggagaggact ccggtatttt tacaacaatt accacaacaa
     7321 aacaaacaac aaacaacatt acaatttact attctagtcg aaatggacac gtatgccggc
     7381 gcttatgata gacaatcaag agaaagagag aactcttctg ccgcctctcc agctacacag
     7441 agatctgcca acgaggataa agctgctgat ttacaaagag aggtagagag agatgggggt
     7501 aggtttagat ttgtgggtca tttctctgag gctcctggta catcagcctt tggtactgct
     7561 gaacggcctg aatttgaaag aatattaaat gagtgtcggg ccggtcgact aaatatgata
     7621 atagtttatg atgtatctag gttcagtaga ctcaaagtta tggatgcaat tccgatcgtg
     7681 agcgagctac ttgcactcgg tgttactatc gtaagtacac aagaaggtgt attcaggcag
     7741 ggtaatgtga tggaccttat acaccttatt atgcgactcg acgctagcca taaagagtca
     7801 tcactgaaga gtgctaaaat cttggacacc aagaacctgc aaagggagct tggaggctat
     7861 gtaggaggaa aagctccgta tggtttcgaa ctcgtgagtg agacaaaaga gattaccaga
     7921 aatggtagga tggtgaacgt ggttattaat aagctagctc atagcacaac ccctctcaca
     7981 gggccttttg agttcgaacc tgacgttatt agatggtggt ggagagagat caaaactcac
     8041 aaacatcttc catttaagcc aggtagccag gctgctatcc accctggatc tatcactggg
     8101 ctttgtaaaa ggatggatgc tgacgcagtg ccaactcgag gggaaacaat cggtaagaag
     8161 actgcatctt ccgcttggga tcccgctaca gttatgagaa tccttcggga tccgcgtatt
     8221 gctggattcg ccgcagaagt catttataag aaaaaacctg atggtactcc tactacaaaa
     8281 atcgaaggtt ataggatcca aagggatcct attactttgc gtccagttga attagactgt
     8341 ggaccgatca tcgaacctgc cgagtggtac gagttgcagg cctggcttga tggtagaggg
     8401 cgtggaaagg gtttatctcg gggacaggca attttgtcag caatggataa gctttattgt
     8461 gagtgtgggg ctgttatgac atcaaaacgt ggagaagagt caatcaagga ttcttacagg
     8521 tgcagacgtc gtaaagtggt ggacccttct gctccaggac agcatgaggg tacatgcaac
     8581 gtttctatgg ctgctcttga taaatttgtg gccgaacgta ttttcaacaa gattagacac
     8641 gctgaagggg atgaggaaac tttagctctt ctttgggagg ctgcccggcg ttttggtaag
     8701 ctaacagagg caccagaaaa aagtggcgaa agagctaatt tggtagccga aagagcagac
     8761 gccttgaatg cattggaaga actttacgaa gatagggctg ccggagcata tgatggacca
     8821 gttggtcgga agcatttcag gaagcaacaa gctgcactaa ctttgagaca gcaaggcgca
     8881 gaggagcgac tcgcagagct tgaggccgcc gaggccccaa agcttccact cgatcaatgg
     8941 tttcctgagg atgccgacgc tgatcctaca ggtccaaaga gctggtgggg aagagctagc
     9001 gtagacgata agagagtgtt tgtgggtcta tttgttgaca agattgtagt tactaagtcc
     9061 actacgggtc gaggacaggg cactcccatc gaaaagagag ccagtattac ctgggcaaag
     9121 cctccaaccg atgatgatga agacgatgca caagatggta ccgaggatgt ggcagcaggt
     9181 tcgacttcta gatctggtac tgctaagaga tctatttggg ctggtgatga agataataag
     9241 cctaagaaga gagaaactta tgctgatgat actgttggta gatttcattc tggttattct
     9301 gaaactaatg aaagaggtaa ggttgttcct gttgctcttg ataagtggag aatttctact
     9361 ggtgaacaat ctgttgctga tgctgttgct caactttttg gtggtactcc tgttgaaaat
     9421 gaagaatcta cttctgaaaa ttttattgat gtttttactg atagacctaa ggttcctgtt
     9481 attattgaag ctgatggtat tcattgggat atgaagcttt ggcttaatgg taagcttaag
     9541 catcattgtg atggttttga ttttgtttct catgctgatg aagaaatgat tggtcaacct
     9601 tgtggttgtc ctaagctttt tgatgaaaga aaggctgctg ctaaggaata tgatgctcct
     9661 aatcctgcta ttactgttac ttttactctt gctgatgatc ctgaacttgg tagatttaag
     9721 tttcaaactg gttcttggac tctttttaag gttcttcatg aagctgaaga tgatgttgaa
     9781 agagttggta agggtggtgc tgttcttgct aatcttgaac ttgaacttgt tgaatatact
     9841 cctaagagag gtcctatgag aaataagctt gtttcttatt ataagcctac tattactgtt
     9901 cttaagtctt ataatgatgc tattgctgat ccaaaaaaaa agaggaaggt gtaggcttcg
     9961 gccatgctag agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt
    10021 ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt
    10081 cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc
    10141 tatcaataaa atttctaatt cctaaaacca aaatccagtg acctcgctgt catgagacga
    10201 attctgacag gatatattgg cgggtaa
//
