LOCUS       sequence-321977-        8188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for expression of omega-dCas9 and sgRNA in E. coli: 
            lacUV2-omega-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and 
            UV5-BsaI-sgRNA (BsaI sites in place of the spacer).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Goldberg GW, Spencer JM, Giganti DO, Camellato BR, Agmon N, Ichikawa
            DM, Boeke JD, Noyes MB
  TITLE     Engineered dual selection for directed evolution of SpCas9 PAM 
            specificity.
  JOURNAL   Nat Commun. 2021 Jan 13;12(1):349. doi: 10.1038/s41467-020-20650-x.
  PUBMED    33441553
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-13"; volume: "12(1):349. doi"; pages: " 
            10.1038/s41467-020-20650-x"
COMMENT     Sequence Label: pGG369
FEATURES             Location/Qualifiers
     source          1..8188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    50..66
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     55..77
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             305..574
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
     CDS             584..607
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(644..670,671..673,674..3160,3161..3163,3164..4747)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4766..4786
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4787..4876
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             4889..4915
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     misc_RNA        5008..5083
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(5095..5119)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      5249..5704
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5336..5355)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5546..5567
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5730..5834
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5835..5903,5904..6695)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6053..6072)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6866..7454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7355..7374
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7608..7625
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7640..7780
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7706..7725)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7866..7888
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7882..8073)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gaattccggg ctttacactt tatgcttccg gctcgcatta tgtgtcgact tgtgagcgga
       61 taacaatttc acacaggaaa cagctatgcc tcaacagcag caaatgcaac ctcccaattc
      121 aagtgcggac aacaaccctt tgcaacagca atcatcacaa aataccgtac caaacgtcct
      181 caaccaaatt aaccaaatct tttctccaga ggagcaacgc agcttattac aagaagccat
      241 cgaaacctgc aagaattttg aaaaaacaca attgtaagtc tcggctccaa gaaggagata
      301 tactatggca cgcgtaactg ttcaggacgc tgtagagaaa attggtaacc gttttgacct
      361 ggtactggtc gccgcgcgtc gcgctcgtca gatgcaggta ggcggaaagg acccgctcgt
      421 accggaagaa aacgataaaa ccactgtaat cgcgctgcgc gaaatcgaag aaggtctgat
      481 caacaaccag atcctcgacg ttcgcgaacg ccaggaacag caagagcagg aagccgctga
      541 attacaagcc gttaccgcta ttgctgaagg tcgtgccgcg gccgactaca aggatgacga
      601 cgacaagttc cggactggtt ccaagacacc cccccacggg accatggata agaaatactc
      661 aataggctta gctatcggca caaatagcgt cggatgggcg gtgatcactg atgaatataa
      721 ggttccgtct aaaaagttca aggttctggg aaatacagac cgccacagta tcaaaaaaaa
      781 tcttataggg gctcttttat ttgacagtgg agagacagcg gaagcgactc gtctcaaacg
      841 gacagctcgt agaaggtata cacgtcggaa gaatcgtatt tgttatctac aggagatttt
      901 ttcaaatgag atggcgaaag tagatgatag tttctttcat cgacttgaag agtctttttt
      961 ggtggaagaa gacaagaagc atgaacgtca tcctattttt ggaaatatag tagatgaagt
     1021 tgcttatcat gagaaatatc caactatcta tcatctgcga aaaaaattgg tagattctac
     1081 tgataaagcg gatttgcgct taatctattt ggccttagcg catatgatta agtttcgtgg
     1141 tcattttttg attgagggag atttaaatcc tgataatagt gatgtggaca aactatttat
     1201 ccagttggta caaacctaca atcaattatt tgaagaaaac cctattaacg caagtggagt
     1261 agatgctaaa gcgattcttt ctgcacgatt gagtaaatca agacgattag aaaatctcat
     1321 tgctcagctc cccggtgaga agaaaaatgg cttatttggg aatctcattg ctttgtcatt
     1381 gggtttgacc cctaatttta aatcaaattt tgatttggca gaagatgcta aattacagct
     1441 ttcaaaagat acttacgatg atgatttaga taatttattg gcgcaaattg gagatcaata
     1501 tgctgatttg tttttggcag ctaagaattt atcagatgct attttacttt cagatatcct
     1561 aagagtaaat actgaaataa ctaaggctcc cctatcagct tcaatgatta aacgctacga
     1621 tgaacatcat caagacttga ctcttttaaa agctttagtt cgacaacaac ttccagaaaa
     1681 gtataaagaa atcttttttg atcaatcaaa aaacggatat gcaggttata ttgatggggg
     1741 agcgagccaa gaagaatttt ataaatttat caaaccaatt ttagaaaaaa tggatggtac
     1801 tgaggaatta ttggtgaaac taaatcgtga agatttgctg cgcaagcaac ggacctttga
     1861 caacggctct attccccatc aaattcactt gggtgagctg cacgctattt tgagaagaca
     1921 agaagacttt tatccatttt taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt
     1981 tcgcatccct tattatgttg gtccattggc gcgtggcaat agtcgttttg catggatgac
     2041 tcggaagtct gaagaaacaa ttaccccgtg gaattttgaa gaagttgtcg ataaaggtgc
     2101 ttcagctcaa tcatttattg aacgcatgac aaactttgat aaaaatcttc caaatgaaaa
     2161 agtactacca aaacatagtt tgctttatga gtattttacg gtttataacg aattgacaaa
     2221 ggtcaaatat gttactgaag gaatgcgaaa accagcattt ctttcaggtg aacagaagaa
     2281 agccattgtt gatttactct tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga
     2341 agattatttc aaaaaaatag aatgttttga tagtgttgaa atttcaggag ttgaagatag
     2401 atttaatgct tcattaggga cataccatga tttgctaaaa attattaaag ataaagattt
     2461 tttggataat gaagaaaatg aagatatctt agaggatatt gttttaacat tgaccttatt
     2521 tgaagatagg gagatgattg aggaaagact taaaacatat gctcacctct ttgatgataa
     2581 ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt
     2641 gattaatggt attagggata agcaatctgg caaaacaata ttagattttt tgaaatcaga
     2701 tggttttgcc aatcgcaatt ttatgcagct gatccatgat gatagtttga catttaaaga
     2761 agacattcaa aaagcacaag tgtctggaca aggcgatagt ttacatgaac atattgcaaa
     2821 tttagctggt agccctgcta ttaaaaaagg tattttacag actgtaaaag ttgttgatga
     2881 attggtcaaa gtaatggggc ggcataagcc agaaaatatc gttattgaaa tggcacgtga
     2941 aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga
     3001 aggtatcaaa gaattaggaa gtcagattct taaagagcat cctgttgaaa atactcaatt
     3061 gcaaaatgaa aagctctatc tctattatct ccaaaatgga agagacatgt atgtggacca
     3121 agaattagat attaatcgtt taagtgatta tgatgtcgat gccattgttc cacaaagttt
     3181 ccttaaagac gattcaatag acaataaggt cttaacgcgt tctgataaaa atcgtggtaa
     3241 atcggataac gttccaagtg aagaagtagt caaaaagatg aaaaactatt ggagacaact
     3301 tctaaacgcc aagttaatca ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg
     3361 aggtttgagt gaacttgata aagctggttt tatcaaacgc caattggttg aaactcgcca
     3421 aatcactaag catgtggcac aaattttgga tagtcgcatg aatactaaat acgatgaaaa
     3481 tgataaactt attcgagagg ttaaagtgat taccttaaaa tctaaattag tttctgactt
     3541 ccgaaaagat ttccaattct ataaagtacg tgagattaac aattaccatc atgcccatga
     3601 tgcgtatcta aatgccgtcg ttggaactgc tttgattaag aaatatccaa aacttgaatc
     3661 ggagtttgtc tatggtgatt ataaagttta tgatgttcgt aaaatgattg ctaagtctga
     3721 gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac tctaatatca tgaacttctt
     3781 caaaacagaa attacacttg caaatggaga gattcgcaag cggccgctaa tcgaaactaa
     3841 tggggaaact ggagaaattg tctgggataa agggcgagat tttgccacag tgcgcaaagt
     3901 attgtccatg ccccaagtca atattgtcaa gaaaacagaa gtacagaccg gtggattctc
     3961 caaggagtca attttaccaa aaagaaattc ggacaagctt attgctcgta aaaaagactg
     4021 ggacccaaaa aaatatggtg gttttgatag tccaacggta gcttattcag tcctagtggt
     4081 tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt tactagggat
     4141 cacaattatg gaaagaagtt cctttgaaaa aaatccgatt gactttttag aagctaaagg
     4201 atataaggaa gttaaaaaag acttaatcat taaactacct aaatatagtc tttttgagtt
     4261 agaaaacggt cgtaaacgga tgctggctag tgccggagaa ttacaaaaag gaaatgagct
     4321 ggctctgcca agcaaatatg tgaatttttt atatttagct agtcattatg aaaagttgaa
     4381 gggtagtcca gaagataacg aacaaaaaca attgtttgtg gagcagcata agcattattt
     4441 agatgagatt attgagcaaa tcagtgaatt ttctaagcgt gttattttag cagatgccaa
     4501 tttagataaa gttcttagtg catataacaa acatagagac aaaccaatac gtgaacaagc
     4561 agaaaatatt attcatttat ttacgttgac gaatcttgga gcgcccgctg cgttcaagta
     4621 cttcgacacc acgatcgatc gtaaacgata tacgtctaca aaagaagttc tagatgccac
     4681 tcttatccat caatccatca ctggtcttta tgaaacacgc attgatttga gtcagctagg
     4741 aggtgacggc ggtggatccg gcggtccgaa gaagaagaga aaggtgtacc cctacgatgt
     4801 tcctgattat gcgggctatc cttatgacgt accagactat gctggtagct acccgtatga
     4861 tgtacccgat tacgctggct ccgctgcacc agccgccaag aagaagaagc tggattttga
     4921 aggtacctaa tagtgagtcg agctgcccgg gttgacacgc aagctcagtc ctaggtataa
     4981 tactagtcga gaccaatctc ggtctcagtt ttagagctag aaatagcaag ttaaaataag
     5041 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt ctacacccag
     5101 ctttcttgta caaagttggc attaactcga ggctagagac tagaaaaagg ccgacaagtc
     5161 ccgctccgct gaagatcctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     5221 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     5281 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5341 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5401 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5461 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5521 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5581 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5641 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5701 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5761 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5821 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5881 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5941 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6001 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6061 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6121 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6181 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6241 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6301 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6361 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6421 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6481 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6541 gtgagcgtgg ttctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6601 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6661 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6721 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6781 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6841 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6901 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6961 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7021 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7081 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7141 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7201 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7261 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7321 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7381 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7441 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7501 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7561 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7621 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7681 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     7741 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     7801 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     7861 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     7921 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     7981 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     8041 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     8101 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     8161 tgatgaacat gcccggttac tggaacgg
//
