LOCUS       sequence-321994-       13443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the constitutive expression of dCas9 fused to TV (TALx6 - 
            VP128) activation domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13443)
  AUTHORS   Selma S, Bernabe-Orts JM, Vazquez-Vilar M, Diego-Martin B, Ajenjo M,
            Garcia-Carpintero V, Granell A, Orzaez D
  TITLE     Strong gene activation in plants with genome-wide specificity using 
            a new orthogonal CRISPR/Cas9-based programmable transcriptional 
            activator.
  JOURNAL   Plant Biotechnol J. 2019 Sep;17(9):1703-1705. doi: 
            10.1111/pbi.13138. Epub 2019 May 23.
  PUBMED    31034138
REFERENCE   2  (bases 1 to 13443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.13138";
            journalName: "Plant Biotechnol J"; date: "2019-09"; volume: "17"; 
            issue: "9"; pages: "1703-1705"
COMMENT     Sequence Label: pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)
FEATURES             Location/Qualifiers
     source          1..13443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        637..981
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     925..945
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1058..1078
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1079..1102,1103..1105,1106..3589,3590..3595,3596..3661,
                     3662..3664,3665..5179)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1079..1102,1103..1105,1106..3592,3593..3595,
                     3596..5179)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1079..5179
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5198..5218
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5222..5242
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6281..6430
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6443..6592
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     terminator      6619..6871
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6906..6924)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7108..7132
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8433..9062
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9491..10564
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10630..10824
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11060..11082)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11168..11308
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11223..11242
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11323..11340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11494..12082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11574..11593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12170..12985)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(12170..12985)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12893..12912
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    13418..13442
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacaagctt cgtctcagtc aggagactag agccaagctg atctcctttg ccccggagat
       61 caccatggac gactttctct atctctacga tctaggaaga aagttcgacg gagaaggtga
      121 cgataccatg ttcaccaccg ataatgagaa gattagcctc ttcaatttca gaaagaatgc
      181 tgacccacag atggttagag aggcctacgc ggcaggtctg atcaagacga tctacccgag
      241 taataatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt
      301 caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca gaagtactat
      361 tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt
      421 ctctaagaaa gtagttccta ctgaatcaaa ggccatggag tcaaaaattc agatcgagga
      481 tctaacagaa ctcgccgtga agactggcga acagttcata cagagtcttt tacgactcaa
      541 tgacaagaag aaaatcttcg tcaacatggt ggagcacgac actctcgtct actccaagaa
      601 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
      661 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
      721 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
      781 agatgcccct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
      841 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
      901 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
      961 ttcatttcat ttggagagga ctccggtatt tttacaacaa ttaccacaac aaaacaaaca
     1021 acaaacaaca ttacaattta ctattctagt cgaaatgccc aagaagaaga ggaaggtgga
     1081 caagaagtac tccattgggc tcgctatcgg cacaaacagc gtcggctggg ccgtcattac
     1141 ggacgagtac aaggtgccga gcaaaaaatt caaagttctg ggcaataccg atcgccacag
     1201 cataaagaag aacctcattg gcgccctcct gttcgactcc ggggaaacgg ccgaagccac
     1261 gcggctcaaa agaacagcac ggcgcagata tacccgcaga aagaatcgga tctgctacct
     1321 gcaggagatc tttagtaatg agatggctaa ggtggatgac tctttcttcc ataggctgga
     1381 ggagtccttt ttggtggagg aggataaaaa gcacgagcgc cacccaatct ttggcaatat
     1441 cgtggacgag gtggcgtacc atgaaaagta cccaaccata tatcatctga ggaagaagct
     1501 tgtagacagt actgataagg ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat
     1561 caaatttcgg ggacacttcc tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga
     1621 caaactcttt atccaactgg ttcagactta caatcagctt ttcgaagaga acccgatcaa
     1681 cgcatccgga gttgacgcca aagcaatcct gagcgctagg ctgtccaaat cccggcggct
     1741 cgaaaacctc atcgcacagc tccctgggga gaagaagaac ggcctgtttg gtaatcttat
     1801 cgccctgtca ctcgggctga cccccaactt taaatctaac ttcgacctgg ccgaagatgc
     1861 caagcttcaa ctgagcaaag acacctacga tgatgatctc gacaatctgc tggcccagat
     1921 cggcgaccag tacgcagacc tttttttggc ggcaaagaac ctgtcagacg ccattctgct
     1981 gagtgatatt ctgcgagtga acacggagat caccaaagct ccgctgagcg ctagtatgat
     2041 caagcgctat gatgagcacc accaagactt gactttgctg aaggcccttg tcagacagca
     2101 actgcctgag aagtacaagg aaattttctt cgatcagtct aaaaatggct acgccggata
     2161 cattgacggc ggagcaagcc aggaggaatt ttacaaattt attaagccca tcttggaaaa
     2221 aatggacggc accgaggagc tgctggtaaa gcttaacaga gaagatctgt tgcgcaaaca
     2281 gcgcactttc gacaatggaa gcatccccca ccagattcac ctgggcgaac tgcacgctat
     2341 cctcaggcgg caagaggatt tctacccctt tttgaaagat aacagggaaa agattgagaa
     2401 aatcctcaca tttcggatac cctactatgt aggccccctc gcccggggaa attccagatt
     2461 cgcgtggatg actcgcaaat cagaagagac tatcactccc tggaacttcg aggaagtcgt
     2521 ggataagggg gcctctgccc agtccttcat cgaaaggatg actaactttg ataaaaatct
     2581 gcctaacgaa aaggtgcttc ctaaacactc tctgctgtac gagtacttca cagtttataa
     2641 cgagctcacc aaggtcaaat acgtcacaga agggatgaga aagccagcat tcctgtctgg
     2701 agagcagaag aaagctatcg tggacctcct cttcaagacg aaccggaaag ttaccgtgaa
     2761 acagctcaaa gaagattatt tcaaaaagat tgaatgtttc gactctgttg aaatcagcgg
     2821 agtggaggat cgcttcaacg catccctggg aacgtatcac gatctcctga aaatcattaa
     2881 agacaaggac ttcctggaca atgaggagaa cgaggacatt cttgaggaca ttgtcctcac
     2941 ccttacgttg tttgaagata gggagatgat tgaagaacgc ttgaaaactt acgctcatct
     3001 cttcgacgac aaagtcatga aacagctcaa gaggcgccga tatacaggat gggggcggct
     3061 gtcaagaaaa ctgatcaatg ggatccgaga caagcagagt ggaaagacaa tcctggattt
     3121 tcttaagtcc gatggatttg ccaaccggaa cttcatgcag ttgatccatg atgactctct
     3181 cacctttaag gaggacatcc agaaagcaca agtttctggc cagggggaca gtctccacga
     3241 gcacatcgct aatcttgcag gtagcccagc tatcaaaaag ggaatactgc agaccgttaa
     3301 ggtcgtggat gaactcgtca aagtaatggg aaggcataag cccgagaata tcgttatcga
     3361 gatggcccga gagaaccaaa ctacccagaa gggacagaag aacagtaggg aaaggatgaa
     3421 gaggattgaa gagggtataa aagaactggg gtcccaaatc cttaaggaac acccagttga
     3481 aaacacccag cttcagaatg agaagctcta cctgtactac ctgcagaacg gcagggacat
     3541 gtacgtggat caggaactgg acatcaatcg gctctccgac tacgacgtgg atgccatcgt
     3601 gccccagtct tttctcaaag atgattctat tgataataaa gtgttgacaa gatccgataa
     3661 aaatagaggg aagagtgata acgtcccctc agaagaagtt gtcaagaaaa tgaaaaatta
     3721 ttggcggcag ctgctgaacg ccaaactgat cacacaacgg aagttcgata atctgactaa
     3781 ggctgaacga ggtggcctgt ctgagttgga taaagccggc ttcatcaaaa ggcagcttgt
     3841 tgagacacgc cagatcacca agcacgtggc ccaaattctc gattcacgca tgaacaccaa
     3901 gtacgatgaa aatgacaaac tgattcgaga ggtgaaagtt attactctga agtctaagct
     3961 ggtttcagat ttcagaaagg actttcagtt ttataaggtg agagagatca acaattacca
     4021 ccatgcgcat gatgcctacc tgaatgcagt ggtaggcact gcacttatca aaaaatatcc
     4081 caagcttgaa tctgaatttg tttacggaga ctataaagtg tacgatgtta ggaaaatgat
     4141 cgcaaagtct gagcaggaaa taggcaaggc caccgctaag tacttctttt acagcaatat
     4201 tatgaatttt ttcaagaccg agattacact ggccaatgga gagattcgga agcgaccact
     4261 tatcgaaaca aacggagaaa caggagaaat cgtgtgggac aagggtaggg atttcgcgac
     4321 agtccggaag gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg aagtacagac
     4381 cggaggcttc tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc tgatcgcacg
     4441 caaaaaagat tgggacccca agaaatacgg cggattcgat tctcctacag tcgcttacag
     4501 tgtactggtt gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa gcgtcaagga
     4561 actgctgggc atcacaatca tggagcgatc aagcttcgaa aaaaacccca tcgactttct
     4621 cgaggcgaaa ggatataaag aggtcaaaaa agacctcatc attaagcttc ccaagtactc
     4681 tctctttgag cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg agctgcagaa
     4741 aggtaacgag ctggcactgc cctctaaata cgttaatttc ttgtatctgg ccagccacta
     4801 tgaaaagctc aaaggatctc ccgaagataa tgagcagaag cagctgttcg tggaacaaca
     4861 caaacactac cttgatgaga tcatcgagca aataagcgaa ttctccaaaa gagtgatcct
     4921 cgccgacgct aacctcgata aggtgctttc tgcttacaat aagcacaggg ataagcccat
     4981 cagggagcag gcagaaaaca ttatccactt gtttactctg accaacttgg gcgcgcctgc
     5041 agccttcaag tacttcgaca ccaccataga cagaaagcgg tacacctcta caaaggaggt
     5101 cctggacgcc acactgattc atcagtcaat tacggggctc tatgaaacaa gaatcgacct
     5161 ctctcagctc ggtggagaca gcagggctga ttcggacccg aagaaaaaga ggaaggtaga
     5221 tcctaagaag aagagaaagg tactgttgga tccgggaaca cctatggacg ctgatttggt
     5281 agcttcttct acagtagtgt gggaacaaga tgcagatcct tttgctggaa cagccgacga
     5341 cttccccgcc ttcaatgaag aggagcttgc atggttgatg gagctgttac ctcaaggagg
     5401 atcagggggt cttttagatc caggtactcc catggatgca gacctggtcg cttcaagtac
     5461 agtcgtttgg gagcaggatg ctgatccatt cgcaggtaca gcagatgact tcccggcttt
     5521 caatgaggag gaacttgctt ggctcatgga attgctcccg caggcaagag gtggatctgg
     5581 tggtctgctc gacccaggaa cgccgatgga cgctgatctg gtagcatcta gtacagtctg
     5641 ggagcaggac gctgacccat tcgctggtac ggctgacgat tttcctgcct ttaatgagga
     5701 agagttagcc tggctgatgg agttattacc ccagggcggt agtggcggat tattggaccc
     5761 tggtacgccg atggatgcag accttgtggc ttcatcaacg gtcgtatggg agcaggatgc
     5821 cgatcctttc gcaggaaccg cagacgactt cccagccttt aacgaagaag agctcgcatg
     5881 gcttatggaa cttttacctc aggcacgtgg aggaagtggt gggctcctgg atccaggcac
     5941 tccgatggac gcagacttag tagctagttc caccgttgtc tgggagcagg atgcagatcc
     6001 ttttgctggt accgcagacg acttccctgc tttcaatgaa gaagaattgg cctggcttat
     6061 ggaattactt ccgcagggtg ggtctggtgg gctgctcgat cccggtacgc cgatggatgc
     6121 tgacttggtt gctagcagta ctgtggtctg ggaacaggat gctgacccct tcgctgggac
     6181 tgccgacgac ttcccagcct tcaacgaaga ggaactcgca tggttgatgg aactgcttcc
     6241 tcaagcacgt ggggggtcag gcggaggcgg atctggcggt gacgcactgg acgactttga
     6301 tctcgacatg cttgggtccg atgctctcga cgattttgac ctcgatatgc ttgggtctga
     6361 cgcattagat gactttgatt tagacatgtt ggggtcagac gctttggacg atttcgactt
     6421 agatatgttg gccagaggta gtgatgccct tgacgacttc gatttagata tgttgggctc
     6481 tgacgccctc gatgacttcg atctggatat gctcggaagt gacgccttgg acgatttcga
     6541 cttggacatg cttgggtcgg acgccctcga cgactttgat ctggatatgt tatgagcttg
     6601 gaatggatct tcgatcccga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     6661 cctgttgccg gtcttgcgac gattatcata taatttctgt tgaattacgt taagcatgta
     6721 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     6781 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     6841 tcgcgcgcgg tgtcatctat gttactagat cgggaattgc caagctaatt cttgaagacg
     6901 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     6961 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7021 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataatg
     7081 ggaccgactc gcgcttgaga cgaagcttga caggatatat tggcgggtaa acctaagaga
     7141 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     7201 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
     7261 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa
     7321 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
     7381 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
     7441 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
     7501 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
     7561 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
     7621 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
     7681 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
     7741 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
     7801 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
     7861 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
     7921 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
     7981 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
     8041 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
     8101 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
     8161 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
     8221 ccgcccgcgc acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
     8281 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
     8341 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
     8401 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
     8461 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
     8521 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
     8581 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
     8641 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
     8701 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
     8761 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
     8821 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
     8881 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
     8941 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
     9001 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
     9061 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
     9121 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
     9181 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
     9241 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
     9301 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
     9361 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
     9421 caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg
     9481 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
     9541 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
     9601 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
     9661 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
     9721 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
     9781 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
     9841 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
     9901 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
     9961 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
    10021 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
    10081 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
    10141 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
    10201 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
    10261 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
    10321 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
    10381 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
    10441 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
    10501 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
    10561 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
    10621 actctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
    10681 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
    10741 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
    10801 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
    10861 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
    10921 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
    10981 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
    11041 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc acagcttgtc
    11101 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    11161 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
    11221 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    11281 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
    11341 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    11401 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    11461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    11521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    11581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    11641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    11701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    11761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    11821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    11881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    11941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    12001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    12061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    12121 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtgat tagaaaaact
    12181 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
    12241 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
    12301 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
    12361 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
    12421 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
    12481 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
    12541 gtcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
    12601 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
    12661 cctggaatgc tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
    12721 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
    12781 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
    12841 catcgggctt cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag
    12901 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag
    12961 acgtttcccg ttgaatatgg ctcataacag aacttattat ttccttcctc ttttctacag
    13021 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
    13081 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
    13141 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc
    13201 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
    13261 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
    13321 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
    13381 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
    13441 aca
//
