LOCUS       sequence-321996-        9519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the constitutive expression of the MS2 coat protein fused on 
            Ct to the activation domain VPR (VP64+p65+Rta).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Selma S, Bernabe-Orts JM, Vazquez-Vilar M, Diego-Martin B, Ajenjo M,
            Garcia-Carpintero V, Granell A, Orzaez D
  TITLE     Strong gene activation in plants with genome-wide specificity using 
            a new orthogonal CRISPR/Cas9-based programmable transcriptional 
            activator.
  JOURNAL   Plant Biotechnol J. 2019 Sep;17(9):1703-1705. doi: 
            10.1111/pbi.13138. Epub 2019 May 23.
  PUBMED    31034138
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.13138";
            journalName: "Plant Biotechnol J"; date: "2019-09"; volume: "17"; 
            issue: "9"; pages: "1703-1705"
COMMENT     Sequence Label: pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)
FEATURES             Location/Qualifiers
     source          1..9519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        637..981
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     925..945
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1061..1447
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             1454..1474
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1478..1498
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1502..1522
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1523..1672
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             1673..1693
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1697..1717
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1718..2074
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             2099..2668
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      2695..2947
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2982..3000)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3184..3208
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4509..5138
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5567..6640
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6706..6900
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7136..7158)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7244..7384
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7299..7318
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7399..7416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7570..8158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7650..7669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8246..9061)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8246..9061)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8969..8988
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9494..9518
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacaagctt cgtctcagtc aggagactag agccaagctg atctcctttg ccccggagat
       61 caccatggac gactttctct atctctacga tctaggaaga aagttcgacg gagaaggtga
      121 cgataccatg ttcaccaccg ataatgagaa gattagcctc ttcaatttca gaaagaatgc
      181 tgacccacag atggttagag aggcctacgc ggcaggtctg atcaagacga tctacccgag
      241 taataatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt
      301 caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca gaagtactat
      361 tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt
      421 ctctaagaaa gtagttccta ctgaatcaaa ggccatggag tcaaaaattc agatcgagga
      481 tctaacagaa ctcgccgtga agactggcga acagttcata cagagtcttt tacgactcaa
      541 tgacaagaag aaaatcttcg tcaacatggt ggagcacgac actctcgtct actccaagaa
      601 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
      661 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
      721 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
      781 agatgcccct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
      841 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
      901 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
      961 ttcatttcat ttggagagga ctccggtatt tttacaacaa ttaccacaac aaaacaaaca
     1021 acaaacaaca ttacaattta ctattctagt cgaaatgatg gcttctaatt ttactcaatt
     1081 tgttcttgtt gataatggtg gtactggtga tgttactgtt gctccttcta attttgctaa
     1141 tggtgttgct gaatggattt cttctaattc tagatctcaa gcttataagg ttacttgttc
     1201 tgttagacaa tcttctgctc aaaatagaaa gtatactatt aaggttgaag ttcctaaggt
     1261 tgctactcaa actgttggtg gtgttgaact tcctgttgct gcttggagat cttatcttaa
     1321 tatggaactt actattccta tttttgctac taattctgat tgtgaactta ttgttaaggc
     1381 tatgcaaggt cttcttaagg atggtaatcc tattccttct gctattgctg ctaattctgg
     1441 tatttattcg gacccaaaaa agaagcgtaa ggtcgatccc aagaagaaga gaaaggtaga
     1501 tcctaagaag aagagaaagg tagacgcatt ggatgacttc gacttagaca tgcttgggtc
     1561 agatgctttg gacgactttg acctcgacat gttgggttct gacgcgctag atgacttcga
     1621 cttggacatg ttgggctccg acgctttgga cgactttgat ctggacatgt taccgaaaaa
     1681 gaaacgcaaa gttgatccta agaagaagag aaaggtacct acacaggccg gcgagggcac
     1741 actgtctgaa gctctgctgc agctgcagtt cgacgacgag gatctgggag ccctgctggg
     1801 aaacagcacc gatcctgccg tgttcaccga cctggccagc gtggacaaca gcgagttcca
     1861 gcagctgctg aaccagggca tccctgtggc ccctcacacc accgagccca tgctgatgga
     1921 ataccccgag gccatcaccc ggctcgtgac aggcgctcag aggcctcctg atccagctcc
     1981 tgcccctctg ggagcaccag gcctgcctaa tggactgctg tctggcgacg aggacttcag
     2041 ctctatcgcc gatatggatt tctcagcctt gctgattaac tctagaagtt ccggatctcg
     2101 ggattccagg gaagggatgt ttttgccgaa gcctgaggcc ggctccgcta ttagtgacgt
     2161 gtttgagggc cgcgaggtgt gccagccaaa acgaatccgg ccatttcatc ctccaggaag
     2221 tccatgggcc aaccgcccac tccccgccag cctcgcacca acaccaaccg gtccagtaca
     2281 tgagccagtc gggtcactga ccccggcacc agtccctcag ccactggatc cagcgcccgc
     2341 agtgactccc gaggccagtc acctgttgga ggatcccgat gaagaaacga gccaggctgt
     2401 caaagccctt cgggagatgg ccgatactgt gattccccag aaggaagagg ctgcaatctg
     2461 tggccaaatg gacctttccc atccgccccc aaggggccat ctggatgagc tgacaaccac
     2521 acttgagtcc atgaccgagg atctgaacct ggactcaccc ctgaccccgg aattgaacga
     2581 gattctggat accttcctga acgacgagtg cctcttgcat gccatgcata tcagcacagg
     2641 actgtccatc ttcgacacat ctctgttttg agcttggaat ggatcttcga tcccgatcgt
     2701 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgacgatt
     2761 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2821 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2881 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2941 ctagatcggg aattgccaag ctaattcttg aagacgaaag ggcctcgtga tacgcctatt
     3001 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     3061 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3121 catgagacaa taaccctgat aaatgcttca ataatgggac cgactcgcgc ttgagacgaa
     3181 gcttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     3241 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     3301 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     3361 cggcttctga cgttcagtgc agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt
     3421 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     3481 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     3541 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     3601 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     3661 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     3721 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     3781 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     3841 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     3901 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     3961 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     4021 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     4081 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     4141 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     4201 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     4261 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg
     4321 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     4381 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     4441 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     4501 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     4561 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     4621 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     4681 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     4741 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     4801 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     4861 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     4921 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     4981 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     5041 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     5101 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     5161 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     5221 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     5281 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     5341 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     5401 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     5461 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
     5521 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     5581 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     5641 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     5701 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     5761 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     5821 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     5881 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     5941 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     6001 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     6061 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     6121 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     6181 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     6241 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     6301 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     6361 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     6421 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     6481 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     6541 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     6601 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     6661 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggactc tttcctgtgg atagcacgta
     6721 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     6781 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     6841 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     6901 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
     6961 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
     7021 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
     7081 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     7141 gacacatgca gctcccggtg acggtcacag cttgtctgta agcggatgcc gggagcagac
     7201 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     7261 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     7321 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     7381 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7441 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7501 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7561 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7621 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7681 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7741 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7801 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7861 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7921 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7981 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8041 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8101 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8161 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8221 gattttggtc atgcattcta ggtgattaga aaaactcatc gagcatcaaa tgaaactgca
     8281 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     8341 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     8401 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     8461 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt
     8521 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     8581 ccaaaccgtt attcattcgt gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa
     8641 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     8701 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga
     8761 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     8821 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     8881 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcggt
     8941 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     9001 catccatgtt ggaatttaat cgcggccttg agcaagacgt ttcccgttga atatggctca
     9061 taacagaact tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
     9121 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
     9181 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
     9241 attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct
     9301 accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata
     9361 gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc
     9421 ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
     9481 gttggctggc tggtggcagg atatattgtg gtgtaaaca
//
