LOCUS       sequence-322007-        9648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for integration of TagRFP-T reporter at AAVS1 safe 
            harbor site via TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Van MV, Fujimori T, Bintu L
  TITLE     Nanobody-mediated control of gene expression and epigenetic memory.
  JOURNAL   Nat Commun. 2021 Jan 22;12(1):537. doi: 10.1038/s41467-020-20757-1.
  PUBMED    33483487
REFERENCE   2  (bases 1 to 9648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-22"; volume: "12(1):537. doi"; pages: " 
            10.1038/s41467-020-20757-1"
COMMENT     Sequence Label: AAV-puro-BGHpA-TRE-pEF-tagRFP-T-NLS
FEATURES             Location/Qualifiers
     source          1..9648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(128..716)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(208..227)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(840..908,909..1700)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1058..1077)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(2061..2080)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2675..2694
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(3097..3399)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3199..3218)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     3522..3544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3536..3553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3537..3553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3560..3579
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3560..3578
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3613..4449
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    4523..4544
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4559..4564,4565..4582,4583..4589)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4597..4613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4621..4637
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4980..4999
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    4998..5053
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5099..5118
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(5224..5244)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5248..5268)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5272..5292)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5302..6033)
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     regulatory      6030..6039
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6061..6079)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     intron          6112..7050
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6135..6155)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    7295..7313
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7331..7349
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7366..7384
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7402..7420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7438..7456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    7529..7753
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7742..7759
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(7779..8378)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7862..7882)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8359..8378
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(8388..8441)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    8465..8490
                     /label=SA
                     /note="splice acceptor site"
     misc_feature    8497..9300
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(9335..9353)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9374..9390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9398..9414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9422..9428,9429..9446,9447..9452))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9467..9488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9605..9622)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
       61 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      121 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      181 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      241 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      301 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      361 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      421 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      481 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      541 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      601 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      661 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      721 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      781 ttttggtcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      841 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      901 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      961 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1021 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1081 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1141 ttgagtattc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1201 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1261 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1321 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1381 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1441 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1501 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1561 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1621 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1681 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1741 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1801 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1861 cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     1921 cgcatcagga aattgtaagc gttaataatt cagaagaact cgtcaagaag gcgatagaag
     1981 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     2041 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     2101 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     2161 ttcggcaagc aggcatcgcc atgcatgggt cacgacgaga tcctcgccgt cgggcatgct
     2221 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     2281 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     2341 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     2401 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     2461 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     2521 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag
     2581 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     2641 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     2701 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     2761 tcatcctgtc tcttgatcag agcttgatcc cctgcgccat cagatccttg gcggcaagaa
     2821 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     2881 ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact
     2941 gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
     3001 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtga aaaggatcta
     3061 ggtgaagatc ctttttgata atctcatgcc tgacatttat attccccaga acatcaggtt
     3121 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
     3181 ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg
     3241 caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac tggccagggg
     3301 gatcaccatc cgtcgccccg gcgtgtcaat aatatcactc tgtacatcca caaacagacg
     3361 ataacggctc tctcttttat aggtgtaaac cttaaactgc cgtacgtata ggctgcgcaa
     3421 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     3481 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     3541 aacgacggcc agtgaattgt aatacgactc actatagggc gaattgaatt tagcggccgc
     3601 gaattcgccc ttagagcaga gccaggaacc cctgtaggga aggggcagga gagccagggg
     3661 catgagatgg tggacgagga agggggacag ggaagcctga gcgcctctcc tgggcttgcc
     3721 aaggactcaa acccagaagc ccagagcagg gccttaggga agcgggaccc tgctctgggc
     3781 ggaggaatat gtcccagata gcactgggga ctctttaagg aaagaaggat ggagaaagag
     3841 aaagggagta gaggcggcca cgacctggtg aacacctagg acgcaccatt ctcacaaagg
     3901 gagttttcca cacggacacc cccctcctca ccacagccct gccaggacgg ggctggctac
     3961 tggccttatc tcacaggtaa aactgacgca cggaggaaca atataaattg gggactagaa
     4021 aggtgaagag ccaaagttag aactcaggac caacttattc tgattttgtt tttccaaact
     4081 gcttctcctc ttgggaagtg taaggaagct gcagcaccag gatcagtgaa acgcaccaga
     4141 cagccgcgtc agagcagctc aggttctggg agagggtagc gcagggtggc cactgagaac
     4201 cgggcaggtc acgcatcccc cccttccctc ccaccccctg ccaagctctc cctcccagga
     4261 tcctctctgg ctccatcgta agcaaacctt agaggttctg gcaaggagag agatggctcc
     4321 aggaaatggg ggtgtgtcac cagataagga atctgcctaa caggaggtgg gggttagacc
     4381 caatatcagg agactaggaa ggaggaggcc taaggatggg gcttttctgt caccaatcct
     4441 gtccctagta aagcttagta ctgtcgatcc gctggcacga caggtttccc gactggaaag
     4501 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4561 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4621 caggaaacag ctatgaccat gattacgcca agcttgggct gcaggtcgag ggatctccat
     4681 aagagaagag ggacagctat gactgggagt agtcaggaga ggaggaaaaa tctggctagt
     4741 aaaacatgta aggaaaattt tagggatgtt aaagaaaaaa ataacacaaa acaaaatata
     4801 aaaaaaatct aacctcaagt caaggctttt ctatggaata aggaatggac agcagggggc
     4861 tgtttcatat actgatgacc tctttatagc caacctttgt tcatggcagc cagcatatgg
     4921 gcatatgttg ccaaactcta aaccaaatac tcattctgat gttttaaatg atttgccctc
     4981 ccatatgtcc ttccgagtga gagacacaaa aaattccaac acactattgc aatgaaaata
     5041 aatttccttt attagccaga agtcagatgc tcaaggggct tcatgatgtc cccataattt
     5101 ttggcagagg gaaaaagatc tcagtggtat ttgtgagcca gggcattggc cacaccagcc
     5161 accaccttct gataggcagc ctgcacctga ggagtgaatt cacgcgtgct gggtctagaa
     5221 ctataccttt ctcttctttt ttggatctac ctttctcttc ttttttggat ctacctttct
     5281 cttctttttt ggatccaatt gcttgtacag ctcgtccatg ccattaagtt tgtgccccag
     5341 tttgctaggg aggtcgcagt atctggccac agccacctcg tgctgctcga cgtaggtctc
     5401 tttgtcggcc tccttgattc tttccagtct gtggtccaca tagtagacgc cgggcatctt
     5461 gaggttctta gcgggtttct tggatctgta tgtggtcttg aagttgcaga tcaggtggcc
     5521 cccgcccacg agcttcaggg ccatgtcggt tctgccttcc aggccgccgt cagcggggta
     5581 cagcatctcg gtgttggcct cccagccgag tgttttcttc tgcatcacag ggccgttgga
     5641 tgggaagttc acccctctga tcttgacgtt gtagatgagg cagccgtcct ggaggctggt
     5701 gtcctgggta gcggtcagca cgcccccgtc ttcgtatgtg gtgactctct cccatgtgaa
     5761 gccctcaggg aaggactgct taaagaaatc ggggatgccc tgggtgtggt tgatgaaggt
     5821 tctgctgccg tacatgaagc tggtagccag gatgtcgaag gcgaagggga gagggccgcc
     5881 ctcgaccacc ttgattctca tggtctgggt gccctcgtag ggcttgcctt cgccctcgga
     5941 tgtgcacttg aagtggtggt tgttcacggt gccctccatg tacagcttca tgtgcatgtt
     6001 ctccttaatc agctcttcgc ccttagacac catggtggcg tggagctcca gcttttgttc
     6061 cctttagtga gggttaattt cgagcttggc gtaatcgcta gctcacgaca cctgaaatgg
     6121 aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg aaccaagatc caaactcaaa
     6181 aagggcaaat tccaaggaga attacatcaa gtgccaagct ggcctaactt cagtctccac
     6241 ccactcagtg tggggaaact ccatcgcata aaacccctcc ccccaaccta aagacgacgt
     6301 actccaaaag ctcgagaact aatcgaggtg cctggacggc gcccggtact ccgtggagtc
     6361 acatgaagcg acggctgagg acggaaaggc ccttttcctt tgtgtgggtg actcacccgc
     6421 ccgctctccc gagcgccgcg tcctccattt tgagctccct gcagcagggc cgggaagcgg
     6481 ccatctttcc gctcacgcaa ctggtgccga ccgggccagc cttgccgccc agggcggggc
     6541 gatacacggc ggcgcgaggc caggcaccag agcaggccgg ccagcttgag actacccccg
     6601 tccgattctc ggtggccgcg ctcgcaggcc ccgcctcgcc gaacatgtgc gctgggacgc
     6661 acgggccccg tcgccgcccg cggccccaaa aaccgaaata ccagtgtgca gatcttggcc
     6721 cgcatttaca agactatctt gccagaaaaa aagcgtcgca gcaggtcatc aaaaatttta
     6781 aatggctaga gacttatcga aagcagcgag acaggcgcga aggtgccacc agattcgcac
     6841 gcggcggccc cagcgcccag gccaggcctc aactcaagca cgaggcgaag gggctcctta
     6901 agcgcaaggc ctcgaactct cccacccact tccaacccga agctcgggat caagaatcac
     6961 gtactgcagc caggtggaag taattcaagg cacgcaaggg ccataacccg taaagaggcc
     7021 aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc cgttgcgaaa
     7081 aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag
     7141 gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc
     7201 ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctcatg
     7261 cattcccacc gtacacgcct acctcgacat acgttctcta tcactgatag ggagtaaact
     7321 cgacatacgt tctctatcac tgatagggat aaactcgaca tacgttctct atcactgata
     7381 gggagtaaac tcgacatacg ttctctatca ctgataggga gtaaactcga catacgttct
     7441 ctatcactga tagggagtaa actcgacgcg tatatcgacg tcttcctagg ctttgctagc
     7501 ccccagctgg ttctttccgc ctcagaagcc atagagccca ccgcatcccc agcatgcctg
     7561 ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa
     7621 tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga
     7681 gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa
     7741 ctagaaggca cagtcgaggc tgatcagcga gctctagatc aggcaccggg cttgcgggtc
     7801 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     7861 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     7921 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     7981 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     8041 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     8101 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     8161 accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     8221 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     8281 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     8341 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatct cgagcctagg gccgggattc
     8401 tcctccacgt caccgcatgt tagaagactt cctctgccct ctccgctgcc agatctctcg
     8461 aggccctgtg ggaggaagag aagaggtcag aagcttgccc cactgtgggg tggaggggac
     8521 agataaaagt acccagaacc agagccacat taaccggccc tgggaatata aggtggtccc
     8581 agctcgggga cacaggatcc ctggaggcag caaacatgct gtcctgaagt ggacataggg
     8641 gcccgggttg gaggaagaag actagctgag ctctcggacc cctggaagat gccatgacag
     8701 ggggctggaa gagctagcac agactagaga ggtaaggggg gtaggggagc tgcccaaatg
     8761 aaaggagtga gaggtgaccc gaatccacag gagaacgggg tgtccaggca aagaaagcaa
     8821 gaggatggag aggtggctaa agccagggag acggggtact ttggggttgt ccagaaaaac
     8881 ggtgatgatg caggcctaca agaaggggag gcgggacgca agggagacat ccgtcggaga
     8941 aggccatcct aagaaacgag agatggcaca ggccccagaa ggagaaggaa aagggaaccc
     9001 agcgagtgaa gacggcatgg ggttgggtga gggaggagag atgcccggag aggacccaga
     9061 cacggggagg atccgctcag aggacatcac gtggtgcagc gccgagaagg aagtgctccg
     9121 gaaagagcat ccttgggcag caacacagca gagagcaagg ggaagaggga gtggaggaag
     9181 acggaacctg aaggaggcgg cagggaagga tctgggccag ccgtagaggt gacccaggcc
     9241 acaagctgca gacagaaagc ggcacaggcc caggggagag aatgctggtc agagaaagca
     9301 aagggcgaat tcgtttaaac ctgcaggact agtcccttta gtgagggtta attctgagct
     9361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     9421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     9481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     9541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     9601 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgag
//
