LOCUS       sequence-322012-        6154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of the cytoplasmic domain of human ATF6with
            an HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Wang Y, Shen J, Arenzana N, Tirasophon W, Kaufman RJ, Prywes R
  TITLE     Activation of ATF6 and an ATF6 DNA binding site by the endoplasmic 
            reticulum stress response.
  JOURNAL   J Biol Chem. 2000 Sep 1. 275(35):27013-20.
  PUBMED    10856300
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Sep 1. 275(35):27013-20."
COMMENT     Sequence Label: pCGN-ATF6 (1-373)
FEATURES             Location/Qualifiers
     source          1..6154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     565..589
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           658..697
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             727..753
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             733..759
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     intron          1913..2485
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(2617..2636)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2682..2737
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2736..2755)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3091..3226
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3332..3349)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3503..4091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3583..3602)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4262..5053,5054..5122))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4885..4904
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5123..5227)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5295..5313
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5351..5373)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5473..5492
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5559..6014)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5696..5717)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5908..5927
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(6140..6154,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(6154,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattc gagctcgccc
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      601 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgggggat
      661 cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc gcgtatggct
      721 tctagctatc cttatgacgt gcctgactat gccagcctgg gaggaccttc tagaatgggg
      781 gagccggctg gggttgccgg caccatggag tcacctttta gcccgggact ctttcacagg
      841 ctggatgaag attgggattc tgctctcttt gctgaactcg gttatttcac agacactgat
      901 gagctgcaat tggaagcagc aaatgagacg tatgaaaaca attttgataa tcttgatttt
      961 gatttggatt tgttaccttg ggagtcagac atttgggaca tcaacaacca aatctgtaca
     1021 gttaaagata ttaaggcaga accccagcca ctttctccag cctcctcaag ttattcagtc
     1081 tcatctcctc ggtcagtgga ctcttattct tcaactcagc atgttcctga ggagttggat
     1141 ttgtcttcta gttctcagat gtctcccctt tccttatatg gtgaaaactc taatagtctc
     1201 tcttcaccgg agccactgaa ggaagataag cctgtcactg gttctaggaa caagactgaa
     1261 aatggactga ctccaaagaa aaaaattcag gtgaattcaa aaccttcaat tcagcccaag
     1321 cctttattgc ttccagcagc acccaagact caaacaaact ccagtgttcc agcaaaaacc
     1381 atcattattc agacagtacc aacgcttatg ccattggcaa agcagcaacc aattatcagt
     1441 ttacaacctg cacccactaa aggccagacg gttttgctgt ctcagcctac tgtggtacaa
     1501 cttcaagcac ctggagttct gccctctgct cagccagtcc ttgctgttgc tgggggagtc
     1561 acacagctcc ctaatcacgt ggtgaatgtg gtaccagccc cttcagcgaa tagcccagtg
     1621 aatggaaaac tttccgtgac taaacctgtc ctacaaagta ccatgagaaa tgtcggttca
     1681 gatattgctg tgctaaggag acagcaacgt ataataaaaa atcgagaatc cgcttgtcag
     1741 tctcgcaaga agaagaaaga atatatgcta gggttagagg cgagattaaa ggctgccctc
     1801 tcagaaaacg agcaactgaa gaaagaaaat ggaacactga agcggcagct ggatgaagtt
     1861 gtgtcagaga accagaggct taaagtccct agtggatcct gagaacttca gggtgagttt
     1921 ggggaccctt gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg
     1981 caaagttttc agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc
     2041 atgataattt tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt
     2101 cttttcattt tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa
     2161 attcactttt gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg
     2221 caatcagggt atattatatt gtacttcagc acagttttag agaacaattg ttataattaa
     2281 atgataaggt agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg
     2341 tagaaacaac tacaccctgg tcatcatcct gcctttctct ttatggttac aatgatatac
     2401 actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt
     2461 tcatgccttc ttctctttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca
     2521 tcattttggc aaagaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     2581 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     2641 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     2701 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     2761 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     2821 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     2881 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     2941 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     3001 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     3061 atccctcgag gagctttttg caaaagccta ggcctccaaa aaagcctctt cactacttct
     3121 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     3181 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     3241 gcgggactat ggttgctgac taattgctgc attaatgaat cggccaacgc gcggggagag
     3301 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3361 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3421 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3481 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3541 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3601 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3661 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3721 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3781 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3841 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3901 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3961 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4021 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4081 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4141 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4201 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4261 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4321 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4381 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4441 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4501 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4561 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4621 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4681 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4741 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4801 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4861 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4921 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4981 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5041 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5101 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5161 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5221 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     5281 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     5341 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     5401 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     5461 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     5521 taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt
     5581 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     5641 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     5701 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     5761 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     5821 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     5881 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     5941 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     6001 gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     6061 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     6121 tgggtaacgc cagggttttc ccagtcacga cgtt
//
