LOCUS       sequence-322017-       16036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for estradiol-inducible expression of the PhiC31 integrase 
            gene and dexamethasone -inducible expression of PhiC31 phage 
            recombination directionality factor (RDF) gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16036)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 16036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pDGB3_alpha2:
            Pnos:ER:lexABD:GAL4AD:T35s-Oplex...
FEATURES             Location/Qualifiers
     source          1..16036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..262
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             304..1239
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             352..1242
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     CDS             1243..1494
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     terminator      1812..2064
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2576..2622
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             2697..4511
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             4512..4532
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4559..4811
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5122..5301
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(6196..6215)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      7448..7700
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    7960..7979
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    8012..8031
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    8064..8083
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    8116..8135
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    8168..8187
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    8220..8239
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        8312..8358
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      9191..9443
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9680..9704
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11005..11634
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12063..13136
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13202..13396
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13632..13654)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13740..13880
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13795..13814
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13895..13912)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14066..14654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14146..14165)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14742..15557)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(14742..15557)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     15465..15484
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    15990..16014
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagatacat gagaattaag ggagtcacgg tatgaccccc gccgatgacg cgggacaagc
       61 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt
      121 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
      181 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
      241 aattcccctc ggtatccaat tagagtctca tattcactct caactcgatc gaggcccatg
      301 tctgctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct
      361 aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat
      421 gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg
      481 atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg
      541 aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct tctagaatgt
      601 gcctggctag agatcctgat gattggactc gtctggcgct ccatggagca cccagggaag
      661 ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg
      721 gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag
      781 ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt
      841 ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag
      901 atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac
      961 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc
     1021 atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg
     1081 gagatgctgg acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag
     1141 gaaacggacc aaagccactt ggccactgcg ggctctactt cgtcgcattc cttgcaaaag
     1201 tattacatca cgggggaggc agagggtttc cctgccacag taatgaaagc gttaacggcc
     1261 aggcaacaag aggtgtttga tctcatccgt gatcacatca gccagacagg tatgccgccg
     1321 acgcgtgcgg aaatcgcgca gcgtttgggg ttccgttccc caaacgcggc tgaagaacat
     1381 ctgaaggcgc tggcacgcaa aggcgttatt gaaattgttt ccggcgcatc acgcgggatt
     1441 cgtctgttgc aggaagagga agaagggttg ccgctggtag gtcgtgtggc tgccggtgaa
     1501 ccggcaggtg gcagtggagg gggcggaagc gggggtacaa attctcaagc gctttcacaa
     1561 ccaattgcct cctctaacgt tcatgataac ttcatgaata atgaaatcac ggctagtaaa
     1621 attgatgatg gtaataattc aaaaccactg tcacctggtt ggacggacca aactgcgtat
     1681 aacgcgtttg gaatcactac agggatgttt aataccacta caatggatga tgtatataac
     1741 tatctattcg atgatgaaga taccccacca aacccaaaaa aagagtgagc ttggaatgga
     1801 tcttcgatcc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     1861 ccggtcttgc gacgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     1921 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     1981 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     2041 cggtgtcatc tatgttacta gatcgggaat tgccaagcta attcttgaag acgaaagggc
     2101 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     2161 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2221 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atgggaccga
     2281 ctcgcgctgt caggagagct tgggctgcag gtcgaggcta aaaaactaat cgcattatca
     2341 tcccctcgac gtactgtaca tataaccact ggttttatat acagcagtac tgtacatata
     2401 accactggtt ttatatacag cagtcgacgt actgtacata taaccactgg ttttatatac
     2461 agcagtactg tacatataac cactggtttt atatacagca gtcgaggtaa gattagatat
     2521 ggatatgtat atggatatgt atatggtggt aatgccatgt aatatgctcc ccttcgcaag
     2581 acccttcctc tatataagga agttcatttc atttggagag gactccggta tttttacaac
     2641 aattaccaca acaaaacaaa caacaaacaa cattacaatt tactattcta gtcgaaatgg
     2701 acacgtatgc cggcgcttat gatagacaat caagagaaag agagaactct tctgccgcct
     2761 ctccagctac acagagatct gccaacgagg ataaagctgc tgatttacaa agagaggtag
     2821 agagagatgg gggtaggttt agatttgtgg gtcatttctc tgaggctcct ggtacatcag
     2881 cctttggtac tgctgaacgg cctgaatttg aaagaatatt aaatgagtgt cgggccggtc
     2941 gactaaatat gataatagtt tatgatgtat ctaggttcag tagactcaaa gttatggatg
     3001 caattccgat cgtgagcgag ctacttgcac tcggtgttac tatcgtaagt acacaagaag
     3061 gtgtattcag gcagggtaat gtgatggacc ttatacacct tattatgcga ctcgacgcta
     3121 gccataaaga gtcatcactg aagagtgcta aaatcttgga caccaagaac ctgcaaaggg
     3181 agcttggagg ctatgtagga ggaaaagctc cgtatggttt cgaactcgtg agtgagacaa
     3241 aagagattac cagaaatggt aggatggtga acgtggttat taataagcta gctcatagca
     3301 caacccctct cacagggcct tttgagttcg aacctgacgt tattagatgg tggtggagag
     3361 agatcaaaac tcacaaacat cttccattta agccaggtag ccaggctgct atccaccctg
     3421 gatctatcac tgggctttgt aaaaggatgg atgctgacgc agtgccaact cgaggggaaa
     3481 caatcggtaa gaagactgca tcttccgctt gggatcccgc tacagttatg agaatccttc
     3541 gggatccgcg tattgctgga ttcgccgcag aagtcattta taagaaaaaa cctgatggta
     3601 ctcctactac aaaaatcgaa ggttatagga tccaaaggga tcctattact ttgcgtccag
     3661 ttgaattaga ctgtggaccg atcatcgaac ctgccgagtg gtacgagttg caggcctggc
     3721 ttgatggtag agggcgtgga aagggtttat ctcggggaca ggcaattttg tcagcaatgg
     3781 ataagcttta ttgtgagtgt ggggctgtta tgacatcaaa acgtggagaa gagtcaatca
     3841 aggattctta caggtgcaga cgtcgtaaag tggtggaccc ttctgctcca ggacagcatg
     3901 agggtacatg caacgtttct atggctgctc ttgataaatt tgtggccgaa cgtattttca
     3961 acaagattag acacgctgaa ggggatgagg aaactttagc tcttctttgg gaggctgccc
     4021 ggcgttttgg taagctaaca gaggcaccag aaaaaagtgg cgaaagagct aatttggtag
     4081 ccgaaagagc agacgccttg aatgcattgg aagaacttta cgaagatagg gctgccggag
     4141 catatgatgg accagttggt cggaagcatt tcaggaagca acaagctgca ctaactttga
     4201 gacagcaagg cgcagaggag cgactcgcag agcttgaggc cgccgaggcc ccaaagcttc
     4261 cactcgatca atggtttcct gaggatgccg acgctgatcc tacaggtcca aagagctggt
     4321 ggggaagagc tagcgtagac gataagagag tgtttgtggg tctatttgtt gacaagattg
     4381 tagttactaa gtccactacg ggtcgaggac agggcactcc catcgaaaag agagccagta
     4441 ttacctgggc aaagcctcca accgatgatg atgaagacga tgcacaagat ggtaccgagg
     4501 atgtggcagc accaaaaaaa aagaggaagg tgtaagcttg gaatggatct tcgatcccga
     4561 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgac
     4621 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     4681 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     4741 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     4801 gttactagat cgggaattgc caagctaatt cttgaagacg aaagggcctc gtgatacgcc
     4861 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4921 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4981 cgctcatgag acaataaccc tgataaatgc ttcaataatg ggaccgactc gcgctgtcag
     5041 gagatacatg agaattaagg gagtcacggt atgacccccg ccgatgacgc gggacaagcc
     5101 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactgagc cgcgggtttc
     5161 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     5221 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     5281 attcccctcg gtatccaatt agagtctcat attcactctc aactcgatcg aggcccatga
     5341 ttcagcaagc cactgcagga gtctcacaag acacttcgga aaatcctaac aaaacaatag
     5401 ttcctgcagc attaccacag ctcaccccta ccttggtgtc actgctggag gtgattgaac
     5461 ccgaggtgtt gtatgcagga tatgatagct ctgttccaga ttcagcatgg agaattatga
     5521 ccacactcaa catgttaggt gggcgtcaag tgattgcagc agtgaaatgg gcaaaggcga
     5581 taccaggctt cagaaactta cacctggatg accaaatgac cctgctacag tactcatgga
     5641 tgtttctcat ggcatttgcc ctgggttgga gatcatacag acaatcaagt ggaaacctgc
     5701 tctgctttgc tcctgatctg attattaatg agcagagaat gtctctaccc tgcatgtatg
     5761 accaatgtaa acacatgctg tttgtctcct ctgaattaca aagattgcag gtatcctatg
     5821 aagagtatct ctgtatgaaa accttactgc ttctctcctc agttcctaag gaaggtctga
     5881 agagccaaga gttatttgat gagattcgaa tgacttatat caaagagcta ggaaaagcca
     5941 tcgtcaaaag ggaagggaac tccagtcaga actggcaacg gttttaccaa ctgacaaagc
     6001 ttctggactc catgcatgag gtggttgaga atctccttac ctactgcttc cagacatttt
     6061 tggataagac catgagtatt gaattcccag agatgttagc tgaaatcatc actaatcaga
     6121 taccaaaata ttcaaatgga aatatcaaaa agcttctgtt tcatcaaaaa tcaatggtga
     6181 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
     6241 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcaa
     6301 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
     6361 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
     6421 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
     6481 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
     6541 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
     6601 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
     6661 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
     6721 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
     6781 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
     6841 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcta
     6901 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
     6961 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
     7021 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
     7081 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtat
     7141 cactggtgaa aagaaaaacc accctggagc caacaaattc tcaagcgctt tcacaaccaa
     7201 ttgcctcctc taacgttcat gataacttca tgaataatga aatcacggct agtaaaattg
     7261 atgatggtaa taattcaaaa ccactgtcac ctggttggac ggaccaaact gcgtataacg
     7321 cgtttggaat cactacaggg atgtttaata ccactacaat ggatgatgta tataactatc
     7381 tattcgatga tgaagatacc ccaccaaacc caaaaaaaga gtgagcttgg aatggatctt
     7441 cgatcccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     7501 tcttgcgacg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     7561 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     7621 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     7681 gtcatctatg ttactagatc gggaattgcc aagctaattc ttgaagacga aagggcctcg
     7741 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     7801 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7861 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatgg gaccgactcg
     7921 cgctgtcagg agttgcgaag atcgatccac tagtctttca attgtgagcg ctcacaattc
     7981 tttctcttcc ctttcttctt tctagtcttt caattgtgag cgctcacaat tctttctctt
     8041 ccctttcttc tttctagtct ttcaattgtg agcgctcaca attctttctc ttccctttct
     8101 tctttctagt ctttcaattg tgagcgctca caattctttc tcttcccttt cttctttcta
     8161 gtctttcaat tgtgagcgct cacaattctt tctcttccct ttcttctttc tagtctttca
     8221 attgtgagcg ctcacaattc tttctcttcc ctttcttctt tctagctcca ccgcggtggc
     8281 ggccggccgt ctagtggatc gattcccctt cgcaagaccc ttcctctata taaggaagtt
     8341 catttcattt ggagaggact ccggtatttt tacaacaatt accacaacaa aacaaacaac
     8401 aaacaacatt acaatttact attctagtcg aaatggctaa gagatctatt tgggctggtg
     8461 atgaagataa taagcctaag aagagagaaa cttatgctga tgatactgtt ggtagatttc
     8521 attctggtta ttctgaaact aatgaaagag gtaaggttgt tcctgttgct cttgataagt
     8581 ggagaatttc tactggtgaa caatctgttg ctgatgctgt tgctcaactt tttggtggta
     8641 ctcctgttga aaatgaagaa tctacttctg aaaattttat tgatgttttt actgatagac
     8701 ctaaggttcc tgttattatt gaagctgatg gtattcattg ggatatgaag ctttggctta
     8761 atggtaagct taagcatcat tgtgatggtt ttgattttgt ttctcatgct gatgaagaaa
     8821 tgattggtca accttgtggt tgtcctaagc tttttgatga aagaaaggct gctgctaagg
     8881 aatatgatgc tcctaatcct gctattactg ttacttttac tcttgctgat gatcctgaac
     8941 ttggtagatt taagtttcaa actggttctt ggactctttt taaggttctt catgaagctg
     9001 aagatgatgt tgaaaaagtt ggtaagggtg gtgctgttct tgctaatctt gaacttgaac
     9061 ttgttgaata tactcctaag agaggtccta tgagaaataa gcttgtttct tattataagc
     9121 ctactattac tgttcttaag tcttataatg atgctattgc tgattaggct tggaatggat
     9181 cttcgatccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     9241 cggtcttgcg acgattatca tataatttct gttgaattac gttaagcatg taataattaa
     9301 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     9361 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     9421 ggtgtcatct atgttactag atcgggaatt gccaagctaa ttcttgaaga cgaaagggcc
     9481 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     9541 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9601 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tgggaccgac
     9661 tcgcgcttga gacgaagctt gacaggatat attggcgggt aaacctaaga gaaaagagcg
     9721 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     9781 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     9841 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt
     9901 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     9961 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
    10021 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
    10081 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
    10141 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
    10201 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
    10261 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
    10321 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
    10381 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
    10441 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
    10501 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
    10561 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
    10621 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
    10681 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
    10741 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
    10801 gcacctctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
    10861 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
    10921 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
    10981 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
    11041 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
    11101 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
    11161 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
    11221 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
    11281 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
    11341 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
    11401 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
    11461 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
    11521 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
    11581 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
    11641 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
    11701 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
    11761 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
    11821 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
    11881 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
    11941 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
    12001 agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
    12061 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
    12121 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
    12181 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
    12241 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
    12301 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
    12361 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
    12421 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
    12481 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
    12541 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
    12601 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
    12661 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
    12721 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
    12781 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
    12841 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
    12901 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
    12961 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
    13021 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
    13081 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
    13141 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggactctttc
    13201 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
    13261 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
    13321 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
    13381 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
    13441 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
    13501 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
    13561 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
    13621 gacggtgaaa acctctgaca catgcagctc ccggtgacgg tcacagcttg tctgtaagcg
    13681 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    13741 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
    13801 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
    13861 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    13921 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    13981 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    14041 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    14101 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    14161 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    14221 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    14281 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    14341 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    14401 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    14461 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    14521 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    14581 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    14641 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    14701 aaaactcacg ttaagggatt ttggtcatgc attctaggtg attagaaaaa ctcatcgagc
    14761 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
    14821 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
    14881 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
    14941 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
    15001 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
    15061 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagtcgaaat
    15121 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
    15181 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
    15241 gctgttttcc ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
    15301 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
    15361 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
    15421 ttcccataca atcggtagat tgtcgcacct gattgcccga cattatcgcg agcccattta
    15481 tacccatata aatcagcatc catgttggaa tttaatcgcg gccttgagca agacgtttcc
    15541 cgttgaatat ggctcataac agaacttatt atttccttcc tcttttctac agtatttaaa
    15601 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
    15661 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
    15721 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
    15781 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
    15841 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
    15901 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
    15961 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacataaca
    16021 agcttcgtct cagtca
//
