LOCUS       sequence-322018-        6443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of human Elk-1 with an HA tag and a 
            mutation in the MAPK phosphorylation site of the transcriptional 
            activation domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Lee SM, Vasishtha M, Prywes R
  TITLE     Activation and repression of cellular immediate early genes by serum
            response factor cofactors.
  JOURNAL   J Biol Chem. 2010 Jul 16. 285(29):22036-49.
  PUBMED    20466732
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Jul 16. 285(29):22036-49."
COMMENT     Sequence Label: pCGN-ELK-1 S383A
FEATURES             Location/Qualifiers
     source          1..6443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     565..589
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           658..697
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             727..753
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             733..759
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     intron          2202..2774
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(2906..2925)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2971..3026
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3025..3044)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3380..3515
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3621..3638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3792..4380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3872..3891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4551..5342,5343..5411))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5174..5193
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5412..5516)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5584..5602
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5640..5662)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5762..5781
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5848..6303)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5985..6006)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6197..6216
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(6429..6443,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(6443,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattc gagctcgccc
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      601 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgggggat
      661 cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc gcgtatggct
      721 tctagctatc cttatgacgt gcctgactat gccagcctgg gaggaccttc tagaatggac
      781 ccatctgtga cgctgtggca gtttctgctg cagctgctga gagagcaagg caatggccac
      841 atcatctcct ggacttcacg ggatggtggt gaattcaagc tggtggatgc agaggaggtg
      901 gcccggctgt ggggactacg caagaacaag accaacatga attacgacaa gctcagccgg
      961 gccttgcggt actactatga caagaacatc atccgcaagg tgagcggcca gaagttcgtc
     1021 tacaagtttg tgtcctaccc tgaggtcgca gggtgctcca ctgaggactg cccgccccag
     1081 ccagaggtgt ctgttacctc caccatgcca aatgtggccc ctgctgctat acatgccgcc
     1141 ccaggggaca ctgtctctgg aaagccaggc acacccaagg gtgcaggaat ggcaggccca
     1201 ggcggtttgg cacgcagcag ccggaacgag tacatgcgct cgggcctcta ttccaccttc
     1261 accatccagt ctctgcagcc gcagccaccc cctcatcctc ggcctgctgt ggtgctcccc
     1321 aatgcagctc ctgcaggggc agcagcgccc ccctcgggga gcaggagcac cagtccaagc
     1381 cccttggagg cctgtctgga ggctgaagag gccggcttgc ctctgcaggt catcctgacc
     1441 ccgcccgagg ccccaaacct gaaatcggaa gagcttaatg tggagccggg tttgggccgg
     1501 gctttgcccc cagaagtgaa agtagaaggg cccaaggaag agttggaagt tgcgggggag
     1561 agagggtttg tgccagaaac caccaaggcc gagccagaag tccctccaca ggagggcgtg
     1621 ccagcccggc tgcccgcggt tgttatggac accgcagggc aggcgggcgg ccatgcggct
     1681 tccagccctg agatctccca gccgcagaag ggccggaagc cccgggacct agagcttcca
     1741 ctcagcccga gcctgctagg tgggccggga cccgaacgga ccccaggatc gggaagtggc
     1801 tccggcctcc aggctccggg gccggcgctg accccatccc tgcttcctac gcatacattg
     1861 accccggtgc tgctgacacc cagctcgctg cctcctagca ttcacttctg gagcaccctg
     1921 gctcccattg cgccccgtag cccggccaag ctctccttcc agtttccatc cagtggcagc
     1981 gcccaggtgc acatcccttc tatcagcgtg gatggcctct cgacccccgt ggtgctctcc
     2041 ccagggcccc agaagccatg actactacca ccaccaccac caccccttct ggggtcactc
     2101 catccatgct ctctccagcc agccatctca aggagaaaca tagttcaact gaaagactca
     2161 tgctctgatt gtggtggggt ggggatcctg agaacttcag ggtgagtttg gggacccttg
     2221 attgttcttt ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca
     2281 gggtgttgtt tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt
     2341 gtttctttca ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt
     2401 ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg
     2461 tttatttgtc agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta
     2521 tattatattg tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta
     2581 gaatatttct gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact
     2641 acaccctggt catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga
     2701 tgaggataaa atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct
     2761 tctctttcct acagctcctg ggcaacgtgc tggttgttgt gctgtctcat cattttggca
     2821 aagaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
     2881 ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca
     2941 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     3001 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     3061 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     3121 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     3181 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     3241 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     3301 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgagg
     3361 agctttttgc aaaagcctag gcctccaaaa aagcctcttc actacttctg gaatagctca
     3421 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     3481 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     3541 gttgctgact aattgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3601 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3661 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3721 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3901 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3961 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4021 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4081 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4141 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4201 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4261 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4321 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4381 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4441 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4501 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4561 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4621 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4681 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4741 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4801 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4861 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4921 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4981 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5041 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5101 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5161 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5221 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5281 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5341 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5401 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5461 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5521 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     5581 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     5641 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     5701 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     5761 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     5821 tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg
     5881 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     5941 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     6001 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     6061 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     6121 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     6181 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     6241 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     6301 cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     6361 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     6421 agggttttcc cagtcacgac gtt
//
