LOCUS       sequence-322019-        8108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the regulated expression of the PhiC31 phage recombination 
            directionality factor (RDF) gene driven by a synthetic promoter 
            consisting of the LacI operator fused to the minimal 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)
FEATURES             Location/Qualifiers
     source          1..8108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    32..51
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    84..103
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    136..155
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    188..207
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    240..259
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    292..311
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        384..430
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1263..1515
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1550..1568)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1752..1776
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3077..3706
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4135..5208
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5274..5468
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5704..5726)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5812..5952
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5867..5886
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5967..5984)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6138..6726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6218..6237)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6814..7629)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6814..7629)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7537..7556
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    8062..8086
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagttgcga agatcgatcc actagtcttt caattgtgag cgctcacaat tctttctctt
       61 ccctttcttc tttctagtct ttcaattgtg agcgctcaca attctttctc ttccctttct
      121 tctttctagt ctttcaattg tgagcgctca caattctttc tcttcccttt cttctttcta
      181 gtctttcaat tgtgagcgct cacaattctt tctcttccct ttcttctttc tagtctttca
      241 attgtgagcg ctcacaattc tttctcttcc ctttcttctt tctagtcttt caattgtgag
      301 cgctcacaat tctttctctt ccctttcttc tttctagctc caccgcggtg gcggccggcc
      361 gtctagtgga tcgattcccc ttcgcaagac ccttcctcta tataaggaag ttcatttcat
      421 ttggagagga ctccggtatt tttacaacaa ttaccacaac aaaacaaaca acaaacaaca
      481 ttacaattta ctattctagt cgaaatggct aagagatcta tttgggctgg tgatgaagat
      541 aataagccta agaagagaga aacttatgct gatgatactg ttggtagatt tcattctggt
      601 tattctgaaa ctaatgaaag aggtaaggtt gttcctgttg ctcttgataa gtggagaatt
      661 tctactggtg aacaatctgt tgctgatgct gttgctcaac tttttggtgg tactcctgtt
      721 gaaaatgaag aatctacttc tgaaaatttt attgatgttt ttactgatag acctaaggtt
      781 cctgttatta ttgaagctga tggtattcat tgggatatga agctttggct taatggtaag
      841 cttaagcatc attgtgatgg ttttgatttt gtttctcatg ctgatgaaga aatgattggt
      901 caaccttgtg gttgtcctaa gctttttgat gaaagaaagg ctgctgctaa ggaatatgat
      961 gctcctaatc ctgctattac tgttactttt actcttgctg atgatcctga acttggtaga
     1021 tttaagtttc aaactggttc ttggactctt tttaaggttc ttcatgaagc tgaagatgat
     1081 gttgaaaaag ttggtaaggg tggtgctgtt cttgctaatc ttgaacttga acttgttgaa
     1141 tatactccta agagaggtcc tatgagaaat aagcttgttt cttattataa gcctactatt
     1201 actgttctta agtcttataa tgatgctatt gctgattagg cttggaatgg atcttcgatc
     1261 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     1321 cgacgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     1381 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     1441 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     1501 ctatgttact agatcgggaa ttgccaagct aattcttgaa gacgaaaggg cctcgtgata
     1561 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1621 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1681 tatccgctca tgagacaata accctgataa atgcttcaat aatgggaccg actcgcgctt
     1741 gagacgaagc ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     1801 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     1861 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     1921 agtgcagtcg gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat gtcgcacaag
     1981 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     2041 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     2101 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     2161 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     2221 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     2281 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     2341 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     2401 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     2461 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     2521 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     2581 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     2641 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     2701 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     2761 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     2821 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacctct
     2881 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     2941 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     3001 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     3061 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     3121 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     3181 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     3241 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     3301 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     3361 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     3421 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     3481 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     3541 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     3601 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     3661 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     3721 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     3781 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     3841 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     3901 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     3961 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     4021 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     4081 ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     4141 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     4201 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     4261 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     4321 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     4381 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     4441 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     4501 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     4561 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     4621 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     4681 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     4741 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     4801 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     4861 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     4921 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     4981 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     5041 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     5101 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     5161 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     5221 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggactctt tcctgtggat
     5281 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     5341 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     5401 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     5461 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     5521 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     5581 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     5641 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     5701 aaacctctga cacatgcagc tcccggtgac ggtcacagct tgtctgtaag cggatgccgg
     5761 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     5821 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     5881 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     5941 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6001 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6061 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6121 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6181 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6241 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6301 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6361 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6421 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6481 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6541 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     6601 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6661 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6721 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6781 cgttaaggga ttttggtcat gcattctagg tgattagaaa aactcatcga gcatcaaatg
     6841 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     6901 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     6961 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     7021 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt
     7081 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     7141 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagtcgaa atacgcgatc
     7201 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     7261 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     7321 ccctgggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     7381 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     7441 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     7501 caatcggtag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     7561 taaatcagca tccatgttgg aatttaatcg cggccttgag caagacgttt cccgttgaat
     7621 atggctcata acagaactta ttatttcctt cctcttttct acagtattta aagatacccc
     7681 aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta
     7741 aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga
     7801 cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc
     7861 aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct
     7921 tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac
     7981 gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
     8041 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacataa caagcttcgt
     8101 ctcagtca
//
