LOCUS       sequence-322020-        9003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the constitutive expression of Streptomyces phage PhiC31 
            integrase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 9003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)
FEATURES             Location/Qualifiers
     source          1..9003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..262
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             297..2111
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             2112..2132
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2159..2411
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2446..2464)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2652..2676
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3977..4606
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5035..6108
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6174..6368
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6604..6626)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6712..6852
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6767..6786
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6867..6884)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7038..7626)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7118..7137)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7714..8529)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7714..8529)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8437..8456
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    8962..8986
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagatacat gagaattaag ggagtcacgg tatgaccccc gccgatgacg cgggacaagc
       61 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt
      121 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
      181 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
      241 aattcccctc ggtatccaat tagagtctca tattcactct caactcgatc gaggcaatgg
      301 acacgtatgc cggcgcttat gatagacaat caagagaaag agagaactct tctgccgcct
      361 ctccagctac acagagatct gccaacgagg ataaagctgc tgatttacaa agagaggtag
      421 agagagatgg gggtaggttt agatttgtgg gtcatttctc tgaggctcct ggtacatcag
      481 cctttggtac tgctgaacgg cctgaatttg aaagaatatt aaatgagtgt cgggccggtc
      541 gactaaatat gataatagtt tatgatgtat ctaggttcag tagactcaaa gttatggatg
      601 caattccgat cgtgagcgag ctacttgcac tcggtgttac tatcgtaagt acacaagaag
      661 gtgtattcag gcagggtaat gtgatggacc ttatacacct tattatgcga ctcgacgcta
      721 gccataaaga gtcatcactg aagagtgcta aaatcttgga caccaagaac ctgcaaaggg
      781 agcttggagg ctatgtagga ggaaaagctc cgtatggttt cgaactcgtg agtgagacaa
      841 aagagattac cagaaatggt aggatggtga acgtggttat taataagcta gctcatagca
      901 caacccctct cacagggcct tttgagttcg aacctgacgt tattagatgg tggtggagag
      961 agatcaaaac tcacaaacat cttccattta agccaggtag ccaggctgct atccaccctg
     1021 gatctatcac tgggctttgt aaaaggatgg atgctgacgc agtgccaact cgaggggaaa
     1081 caatcggtaa gaagactgca tcttccgctt gggatcccgc tacagttatg agaatccttc
     1141 gggatccgcg tattgctgga ttcgccgcag aagtcattta taagaaaaaa cctgatggta
     1201 ctcctactac aaaaatcgaa ggttatagga tccaaaggga tcctattact ttgcgtccag
     1261 ttgaattaga ctgtggaccg atcatcgaac ctgccgagtg gtacgagttg caggcctggc
     1321 ttgatggtag agggcgtgga aagggtttat ctcggggaca ggcaattttg tcagcaatgg
     1381 ataagcttta ttgtgagtgt ggggctgtta tgacatcaaa acgtggagaa gagtcaatca
     1441 aggattctta caggtgcaga cgtcgtaaag tggtggaccc ttctgctcca ggacagcatg
     1501 agggtacatg caacgtttct atggctgctc ttgataaatt tgtggccgaa cgtattttca
     1561 acaagattag acacgctgaa ggggatgagg aaactttagc tcttctttgg gaggctgccc
     1621 ggcgttttgg taagctaaca gaggcaccag aaaaaagtgg cgaaagagct aatttggtag
     1681 ccgaaagagc agacgccttg aatgcattgg aagaacttta cgaagatagg gctgccggag
     1741 catatgatgg accagttggt cggaagcatt tcaggaagca acaagctgca ctaactttga
     1801 gacagcaagg cgcagaggag cgactcgcag agcttgaggc cgccgaggcc ccaaagcttc
     1861 cactcgatca atggtttcct gaggatgccg acgctgatcc tacaggtcca aagagctggt
     1921 ggggaagagc tagcgtagac gataagagag tgtttgtggg tctatttgtt gacaagattg
     1981 tagttactaa gtccactacg ggtcgaggac agggcactcc catcgaaaag agagccagta
     2041 ttacctgggc aaagcctcca accgatgatg atgaagacga tgcacaagat ggtaccgagg
     2101 atgtggcagc accaaaaaaa aagaggaagg tgtaagcttg gaatggatct tcgatcccga
     2161 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgac
     2221 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     2281 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     2341 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     2401 gttactagat cgggaattgc caagctaatt cttgaagacg aaagggcctc gtgatacgcc
     2461 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     2521 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2581 cgctcatgag acaataaccc tgataaatgc ttcaataatg ggaccgactc gcgctgtcat
     2641 gagacgaatt ctgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     2701 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     2761 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     2821 agtgcagtcg gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat gtcgcacaag
     2881 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     2941 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     3001 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     3061 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     3121 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     3181 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     3241 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     3301 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     3361 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     3421 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     3481 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     3541 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     3601 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     3661 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     3721 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacctct
     3781 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     3841 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     3901 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     3961 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     4021 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     4081 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     4141 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     4201 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     4261 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     4321 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     4381 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     4441 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     4501 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     4561 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     4621 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     4681 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     4741 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     4801 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     4861 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     4921 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     4981 ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     5041 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     5101 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     5161 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     5221 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     5281 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     5341 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     5401 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     5461 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     5521 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     5581 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     5641 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     5701 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     5761 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     5821 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     5881 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     5941 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     6001 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     6061 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     6121 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggactctt tcctgtggat
     6181 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     6241 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     6301 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     6361 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     6421 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     6481 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     6541 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     6601 aaacctctga cacatgcagc tcccggtgac ggtcacagct tgtctgtaag cggatgccgg
     6661 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     6721 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     6781 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     6841 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6901 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6961 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7021 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7081 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7141 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7201 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7261 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7321 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7381 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7441 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7501 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7561 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7621 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7681 cgttaaggga ttttggtcat gcattctagg tgattagaaa aactcatcga gcatcaaatg
     7741 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     7801 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     7861 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     7921 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt
     7981 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     8041 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagtcgaa atacgcgatc
     8101 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     8161 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     8221 ccctgggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     8281 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     8341 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     8401 caatcggtag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     8461 taaatcagca tccatgttgg aatttaatcg cggccttgag caagacgttt cccgttgaat
     8521 atggctcata acagaactta ttatttcctt cctcttttct acagtattta aagatacccc
     8581 aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta
     8641 aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga
     8701 cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc
     8761 aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct
     8821 tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac
     8881 gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
     8941 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacataa cgaattcgtc
     9001 tca
//
