LOCUS       sequence-322022-        9211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the regulated expression of the PhiC31 phage integrase gene 
            driven by a synthetic promoter consisting of the LacI operator fused
            to the minimal 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)
FEATURES             Location/Qualifiers
     source          1..9211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    32..51
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    84..103
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    136..155
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    188..207
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    240..259
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    292..311
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        384..430
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             505..2319
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             2320..2340
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2367..2619
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2654..2672)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2860..2884
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4185..4814
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5243..6316
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6382..6576
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6812..6834)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6920..7060
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6975..6994
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7075..7092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7246..7834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7326..7345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7922..8737)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7922..8737)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8645..8664
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9170..9194
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagttgcga agatcgatcc actagtcttt caattgtgag cgctcacaat tctttctctt
       61 ccctttcttc tttctagtct ttcaattgtg agcgctcaca attctttctc ttccctttct
      121 tctttctagt ctttcaattg tgagcgctca caattctttc tcttcccttt cttctttcta
      181 gtctttcaat tgtgagcgct cacaattctt tctcttccct ttcttctttc tagtctttca
      241 attgtgagcg ctcacaattc tttctcttcc ctttcttctt tctagtcttt caattgtgag
      301 cgctcacaat tctttctctt ccctttcttc tttctagctc caccgcggtg gcggccggcc
      361 gtctagtgga tcgattcccc ttcgcaagac ccttcctcta tataaggaag ttcatttcat
      421 ttggagagga ctccggtatt tttacaacaa ttaccacaac aaaacaaaca acaaacaaca
      481 ttacaattta ctattctagt cgaaatggac acgtatgccg gcgcttatga tagacaatca
      541 agagaaagag agaactcttc tgccgcctct ccagctacac agagatctgc caacgaggat
      601 aaagctgctg atttacaaag agaggtagag agagatgggg gtaggtttag atttgtgggt
      661 catttctctg aggctcctgg tacatcagcc tttggtactg ctgaacggcc tgaatttgaa
      721 agaatattaa atgagtgtcg ggccggtcga ctaaatatga taatagttta tgatgtatct
      781 aggttcagta gactcaaagt tatggatgca attccgatcg tgagcgagct acttgcactc
      841 ggtgttacta tcgtaagtac acaagaaggt gtattcaggc agggtaatgt gatggacctt
      901 atacacctta ttatgcgact cgacgctagc cataaagagt catcactgaa gagtgctaaa
      961 atcttggaca ccaagaacct gcaaagggag cttggaggct atgtaggagg aaaagctccg
     1021 tatggtttcg aactcgtgag tgagacaaaa gagattacca gaaatggtag gatggtgaac
     1081 gtggttatta ataagctagc tcatagcaca acccctctca cagggccttt tgagttcgaa
     1141 cctgacgtta ttagatggtg gtggagagag atcaaaactc acaaacatct tccatttaag
     1201 ccaggtagcc aggctgctat ccaccctgga tctatcactg ggctttgtaa aaggatggat
     1261 gctgacgcag tgccaactcg aggggaaaca atcggtaaga agactgcatc ttccgcttgg
     1321 gatcccgcta cagttatgag aatccttcgg gatccgcgta ttgctggatt cgccgcagaa
     1381 gtcatttata agaaaaaacc tgatggtact cctactacaa aaatcgaagg ttataggatc
     1441 caaagggatc ctattacttt gcgtccagtt gaattagact gtggaccgat catcgaacct
     1501 gccgagtggt acgagttgca ggcctggctt gatggtagag ggcgtggaaa gggtttatct
     1561 cggggacagg caattttgtc agcaatggat aagctttatt gtgagtgtgg ggctgttatg
     1621 acatcaaaac gtggagaaga gtcaatcaag gattcttaca ggtgcagacg tcgtaaagtg
     1681 gtggaccctt ctgctccagg acagcatgag ggtacatgca acgtttctat ggctgctctt
     1741 gataaatttg tggccgaacg tattttcaac aagattagac acgctgaagg ggatgaggaa
     1801 actttagctc ttctttggga ggctgcccgg cgttttggta agctaacaga ggcaccagaa
     1861 aaaagtggcg aaagagctaa tttggtagcc gaaagagcag acgccttgaa tgcattggaa
     1921 gaactttacg aagatagggc tgccggagca tatgatggac cagttggtcg gaagcatttc
     1981 aggaagcaac aagctgcact aactttgaga cagcaaggcg cagaggagcg actcgcagag
     2041 cttgaggccg ccgaggcccc aaagcttcca ctcgatcaat ggtttcctga ggatgccgac
     2101 gctgatccta caggtccaaa gagctggtgg ggaagagcta gcgtagacga taagagagtg
     2161 tttgtgggtc tatttgttga caagattgta gttactaagt ccactacggg tcgaggacag
     2221 ggcactccca tcgaaaagag agccagtatt acctgggcaa agcctccaac cgatgatgat
     2281 gaagacgatg cacaagatgg taccgaggat gtggcagcac caaaaaaaaa gaggaaggtg
     2341 taagcttgga atggatcttc gatcccgatc gttcaaacat ttggcaataa agtttcttaa
     2401 gattgaatcc tgttgccggt cttgcgacga ttatcatata atttctgttg aattacgtta
     2461 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     2521 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     2581 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattgcca agctaattct
     2641 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     2701 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     2761 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2821 caataatggg accgactcgc gctgtcatga gacgaattct gacaggatat attggcgggt
     2881 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     2941 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
     3001 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     3061 gtcatctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     3121 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     3181 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     3241 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     3301 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     3361 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     3421 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     3481 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     3541 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     3601 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     3661 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     3721 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     3781 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     3841 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     3901 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     3961 tacgtgttcg agccgcccgc gcacctctca accgtgcggc tgcatgaaat cctggccggt
     4021 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     4081 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     4141 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     4201 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     4261 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     4321 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     4381 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     4441 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     4501 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     4561 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     4621 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     4681 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     4741 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     4801 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     4861 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     4921 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     4981 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     5041 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     5101 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     5161 cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa
     5221 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     5281 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     5341 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     5401 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     5461 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     5521 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     5581 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     5641 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     5701 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     5761 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     5821 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     5881 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     5941 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     6001 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     6061 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     6121 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     6181 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     6241 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     6301 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     6361 aggtcgaaaa ggactctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     6421 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     6481 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     6541 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     6601 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     6661 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     6721 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     6781 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggtgacgg
     6841 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     6901 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     6961 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     7021 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     7081 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7141 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7201 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7261 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7321 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7381 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7441 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7501 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7561 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7621 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7681 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7741 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7861 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggtg
     7921 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     7981 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     8041 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     8101 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     8161 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     8221 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     8281 gcgcctgagc gagtcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     8341 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     8401 attcttctaa tacctggaat gctgttttcc ctgggatcgc agtggtgagt aaccatgcat
     8461 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     8521 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     8581 acaactctgg cgcatcgggc ttcccataca atcggtagat tgtcgcacct gattgcccga
     8641 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     8701 gccttgagca agacgtttcc cgttgaatat ggctcataac agaacttatt atttccttcc
     8761 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
     8821 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
     8881 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
     8941 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
     9001 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
     9061 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
     9121 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
     9181 attgtggtgt aaacataacg aattcgtctc a
//
