LOCUS       sequence-322023-       12262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12262)
  AUTHORS   Valente EM, Silhavy JL, Brancati F, Barrano G, Krishnaswami SR, 
            Castori M, Lancaster MA, Boltshauser E, Boccone L, Al-Gazali L, 
            Fazzi E, Signorini S, Louie CM, Bellacchio E, Bertini E, 
            Dallapiccola B, Gleeson JG
  TITLE     Mutations in CEP290, which encodes a centrosomal protein, cause 
            pleiotropic forms of Joubert syndrome.
  JOURNAL   Nat Genet. 2006 Jun . 38(6):623-5.
  PUBMED    16682970
REFERENCE   2  (bases 1 to 12262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2006 Jun . 38(6):623-5."
COMMENT     Sequence Label: pEGFP-mCep290
FEATURES             Location/Qualifiers
     source          1..12262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        764..1067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1068..1271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1222..1242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1310..1319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1316..1318,1319..1321,1322..2032)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1316..1318,1319..1321,1322..2032)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1361..1382)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1622..1641)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1969..1990
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3833..3844
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    9753..9874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9790..9809)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9844..9863
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9881..10336)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10018..10039)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10230..10249
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10363..10467
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10469..10826
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10469..10660
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10492..10512)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10677..10812
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10861..11655
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10861..11655
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10915..10934)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11525..11544
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11843..11862)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11887..11934
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
      721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1261 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt
     1321 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1381 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1441 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1501 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1561 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1621 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1681 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1741 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1801 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1861 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1921 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1981 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtactcaga
     2041 tctcgagcgg ccgccagtgg gatggatatc tgcagaattc gcccttatgc cacctaatat
     2101 aaagtggaaa gaattaatca aagttgatcc agatgacctg ccacggcaag aagagttagc
     2161 agataaatta ttgatatctt tatccaaggt ggaagtaaat gaactaaaaa atgaagacca
     2221 agaaaacatg atacatctat tcagaattac ccagtctcta atgaagatga aagcccagga
     2281 agtagagctc gctttggaag aagttgaaaa ggctggagaa gaacaagcaa aatttgaaaa
     2341 tcaattaaag acaaaagtaa tgaaactgga aaatgaactg gagatggctc agcagtctgc
     2401 agggggacgt gacactcggt ttttacgtga cgaaattcgc caacttgaga agcagctgga
     2461 acaaaaagat agagaattag aggatatgga aaaagaattg gataaagaaa agaaggttaa
     2521 tgaacaattg gctcttcgaa atgaggaggc agaaaatgaa aacagcaaat taagaagaga
     2581 gaatgagcag cttcggcagg acattattga ctaccagaaa caaatagatt cacagaaaga
     2641 atcacttctg tcaaggagag gagaagacag tgactaccga tcacagttgt ctaaaaagaa
     2701 ctatgaactt gttcaatatc tggacgaaat acagacctta acagaagcta atgagaagat
     2761 tgaagttcag aaccaagaaa tgaggaaaaa cctggaagag tctgtgcagg agatggagaa
     2821 gatgactgat gagtacaaca ggatgaaagc gcttgtgcat cagtcggatg ctgtcatgga
     2881 ccagatcaag aaggagaatg agcactatcg cctgcaagtt cgagagctca cggatcttct
     2941 gaaggcgaag gatgaagagg acgatccagt catgatggct gttaacgcaa aagtggaaga
     3001 gtggaagtta attttgtctt ctaaagatga tgaaatcatt gaatatcagc aaatgctaca
     3061 gagtctgaga gggaaactta aaaatgccca acttgatgca gacaaaagta acatcatggc
     3121 tctgaaacag ggtatccagg agcgagacag tcaaattaag atgcttactg agcaagtaga
     3181 acagtatacg aaagaaatgg aaaaaaatac gtttattatt gaagatttga aaaatgaact
     3241 ccaaaaagac aaaggtactt caaactttta tcagcagact cattatatga aaattcactc
     3301 aaaagtacaa attttagaag agaaaacaaa agaggccgag agaatagctg agctggctga
     3361 ggctgatgcc agggagaagg acaaagaatt ggttgaggct ctgaagagat taaaagatta
     3421 tgaatctgga gtatatggct tagaagatgc tgttatcgaa ataaagaatt gtaaagccca
     3481 aattaaaata agagatggag agatggaagt gttgaccaag gagatcaata agcttgagat
     3541 gaagatcaat gacatccttg atgaaaacga agcccttaga gagcgggctg gccttgaacc
     3601 caagacaatg attgatttga ctgaatttag aaacagcaaa cggttaaagc agcaacagta
     3661 cagagctgag aaccaggttc ttttgaaaga gatcgaaagt ctagaggagg agcgtcttga
     3721 cttgaaaaga aagattcgtc aaatggctca agaaagaggc aaaaggaacg cagcctcagg
     3781 attaaccatt gacgacttga acttatctga aaccttttct catgaaaata aaatagaagg
     3841 aagaaaacta aattttatga gcctcaataa tatgaatgaa acacaatcaa agaatgagtt
     3901 tctttcaaga gaattggctg aaaaggaaaa agatttagaa agaagtagga cagtaattgc
     3961 taaattccag agtaaactaa aagaattagt cgaagaaaat aagcaacttg aagaaggtat
     4021 gaaagaaatt ttgcaagcta ttaaggacat gccgaaggat tctgatgtga aaggaggtga
     4081 aacatcttta atcatcccga gtcttgaaag actggttaac gctatggaat caaagaatgc
     4141 agaaggaatc ttcgatgcca gcttgcatct aaaagcccaa gttgaccaac ttacaggaag
     4201 gaatgaagaa ttaaggcaag aactcaggca atctcggaaa gaggctgtaa attattcaca
     4261 gcagttggta aaagcaaact taaagattga ccaccttgag aaggaaactg accttctacg
     4321 tcagtctgca ggctccaatg tagtatacaa aggcatagac ttacccgatg ggatagcacc
     4381 atccagtgcc tatatcatca attctcagaa tgaatactta atacatcttt tgcaggaact
     4441 agacaataaa gaaaagaaat taaaacattt agaagattcc cttgaagatt ataacagaaa
     4501 gtttgcagtg attcgtcatc aacaaagttt attatataaa gaatacctaa gtgaaaagga
     4561 tatttggaaa acagactctg agatgataag agaggagaag agaaaactgg aagatcaagc
     4621 tgagcaggat gctgtgaaag taaaagagta caacaatttg ctcagtgctc ttcagatgga
     4681 ttcgaacgag atgaagaaaa tgctctcaga aaacagtagg aaaatcaccg ttctgcaagt
     4741 gaacgagaag tccctcatcc ggcagtacac caccttagtg gagatggagc ggcacctgag
     4801 aaaggaaaac gggaaacaca ggaatgacgt catagccatg gaggccgaag tcactgaaaa
     4861 acttggaagt ttgcaaagat tcaaggaaat ggccatcttc aagattgcag ctcttcagaa
     4921 ggttatagat aatagtgttt ctttgtctga attagaacta gccaataaac agtacaatga
     4981 gctgactact aagtacaggg acatcttgca gaaagataat atgcttgttc aaagaacaag
     5041 taacttagaa cacctggagt gtgagaatgc atcgctaaaa gaacagatgg aggctatcag
     5101 taaagagctg gagattacaa aggaaaaact ccataccatt gaacaggcct gggaacaaga
     5161 aacgaagtta ggaaatgact caaacatgga taaggctaag aaatcaatga caaacagtga
     5221 cattgtttct atttcaaaga aaatcactgt gttggagatg aaggaattaa atgaaaggca
     5281 gagggctgaa cactgtcaga aaatgtatga gcacttaagg acttcattaa agcaaatgga
     5341 agaacgtaat tttgaattgg aaaccaagtt cactgagctt actaaaatca acctggatgc
     5401 acaaaaggtg gagcagatgt tgagagacga attagctgat agtgtgacca aggcagtaag
     5461 tgatgctgac cgacagcgga ttctagaact agagaagagt gaagtggagc tcaaagttga
     5521 agtgtccaag ctgagagaga tttcagatat tgccaaaaga caagttgatt ttttgaattc
     5581 gcaacaacag tccagggaaa aggaagtgga atccctcaga acgcagctgc tggacttcca
     5641 ggcacaatct gacgaaaagg ctctaattgc caaattgcac caacatgttg tctctcttca
     5701 aattagtgag gccactgccc tcggtaagtt agagtcagtt acgtccaaac tccagaagat
     5761 ggaagcctac aatttgcgct tagaacagaa actggatgaa aaagagcagg cgctctacta
     5821 tgctcgtttg gaaggtagaa acagagcaaa acacctgcgc caaaccattc agtcgcttcg
     5881 aagacagttc agtggagctc tacccttagc acagcaggaa aagttctcca aaacgatgat
     5941 tcagttgcaa aatgacaaac ttaagataat gcaagaaatg aagaattcgc aacaggaaca
     6001 cagaaatatg gaaaacaaaa cactggagtt ggaattaaaa ttaaaaggct tagaagaatt
     6061 gatcagtact ttaaaggatg ccaggggagc ccagaaggta atcaattggc atgtgaaaat
     6121 agaagaactt cgcctccaag aacttaagct aaatagagaa ctagtcaagg gtaaagaaga
     6181 aatcaaatat ttgaataata tcatctctga atatgagcat acaatcaaca gtctagagga
     6241 agaaattgtt cagcaaagca agttccatga agaaagacag atggcttggg atcaaagaga
     6301 agttgagctg gaacgccagt tagacatttt tgatcatcag caaaatgaaa tactcagtgc
     6361 agcacaaaag tttgaagact ctacaggatc aatgccagac cccagcttgc ctcttccaaa
     6421 ccaacttgaa attgctctaa gaaaaattaa ggagaatatt caagtaattc ttaaaacaca
     6481 agcaacttgc aagtcactag aagagaaact aaaagaaaaa gaatctgctt tacggttggc
     6541 agagcaaaat attctgtcaa gagacaaagt aatcaatgaa ctgaggcttc gattgcctgc
     6601 cacggctgat cgagaaaaac ttatagctga gctagaaaga aaagagctgg agccgaaatc
     6661 tcatcacaca atgaaaattg cccaccaaac tattgccaac atgcaggcaa ggttaaatca
     6721 caaggaagaa gtattgaaga aataccagca ccttctggag aaggccagag aggagcaaag
     6781 agaaattgtt aagaagcatg aggaagacct tcatgttctt catcacaaat tagaacaaca
     6841 ggccgataat tcactcaata aattcagaca gacagctcag gatttactta agcagtctcc
     6901 tgctccagtt cccaccaaca aacatttcat tcgtctggcc gagatggagc agacagtagc
     6961 agaacaagat gactctctgt cctcactttt gaccaaacta aagaaagtat caaaagattt
     7021 ggaaaaacaa aaagaaatca ctgagttaaa agtcagagag tttgaaaata ccaaactacg
     7081 gctccaagaa actcatgcca gtgaggtaaa gaaagtgaaa gcagaggtag aggacttaag
     7141 gcatgctcta gcccaagcac acaaggactc ccagagttta aagtctgaac tccaggctca
     7201 gaaagaagca aactccagag ctccaacaac cacaatgagg aatcttgtag acaggctaaa
     7261 gagccaacta gccttgaaag agaagcaaca aaaggcactt agtcgagccc tgttggaact
     7321 tcggtcggaa atgacagcag cagctgagga acgtataatc gctgtaactt ctcaaaaaga
     7381 ggcaaatctc aatgttcaac aagttgttga gcgccatact agagagctaa agtcacaaat
     7441 tgaagattta aatgaaaatc ttttaaaatt gaaagaagct cttaaaacaa gtaagaacaa
     7501 agaaaattca ctagctgatg atttaaatga attaaataat gaactgcaaa aaaagcaaaa
     7561 agcttataat aaaatcctta gagagaaaga tggaattgat caagaaaatg atgaactgag
     7621 aagacagatt aaaagactgt ccagtggact gcagagcaaa actttgatag ataacaagca
     7681 aagtttaatc gatgaacttc aaaagaaagt taaaaaactt gaaagccaac tggaaagaaa
     7741 ggtggatgac gtagacataa agccggtgaa ggaaaagagt agtaaagaag aattaattag
     7801 gtgggaagaa ggtaagaaat ggcaaaccaa agtagaggga ctacgaaaca gactaaagga
     7861 gaaggaagga gaagcccacg gcctggcaaa gcagctgaat accttaaagg aactttttgc
     7921 caaagctgat aaagagaaac ttactttgca gaagaaactg aaaacaacag gaatgactgt
     7981 tgaccaggtt ttaggagtgc gagctttgga atctgaaaaa gagttggaag agctaaaaaa
     8041 gaaaaatctg gacctagaaa atgacatatt atacatgagg acccagcagg ctcttccacg
     8101 agattctgtt gtggaagact tacatttaca aaataaatac cttcaagaaa aacttcatac
     8161 tttagaaaaa aaactttcaa aggagaaata ttctcagtct ttgacttcag aaatagagtc
     8221 agatgatcac tgtcaaaaag aacaagaact tcagaaggaa aatttgaagt tgtcatctga
     8281 aaacatcgag ctgaaatttc aacttgaaca agcaaataaa gatttgccaa gactaaagaa
     8341 tcaagtgaaa gatttgaagg aaatgtgtga atttcttaag aaaggaaaac tggaacttga
     8401 gcggaagctt ggtcaggtca gaggggctgg tagaagtggg aagacaatcc cagaactaga
     8461 aaaaaccatt gggttaatga agaaagtagt tgaaaaagtc caaagagaaa atgaacaatt
     8521 gaaaaaggca tcaggaatac tgactagtga aaaaatggct actattgagg aagaaaatag
     8581 aaacttaaag gctgaactag aaaagcttaa agctcacttt ggacgtcagt tgagtatgca
     8641 gtttgaatct aagaacaaag gtactgagaa aattgttgcc gaaaatgaac ggcttcggaa
     8701 agaacttaag aaagaaatag aagcctctga gaaactgcgg atagctaaga acaacttaga
     8761 gctggtgaac gacaagatgg cagctcaact cgaagaaact gggaagagac tacagtttgc
     8821 agaaagtaga gccccacagc tggaaggtgc tgacagcaag agctggaagt caattgtggt
     8881 ctcaagagtg tatgagacca agatgaaaga gcttgaaagt gacattgcca aaaagaatca
     8941 aagtatcact gaccttaaac agcttgtaag agaagcaaca gagagagaac agaaagctaa
     9001 gaaatacact gaagaccttg aacaacagat tgagatcctc aaaaatgttc ctgaaggtgc
     9061 cgagacagag caagagctta tacgggaact ccagcttctt agattagcca ataatcagat
     9121 ggataaagaa agggcagaat taatccatca gatagaaatt aacaaggacc aaaccagagc
     9181 tgacagtagc atacctgatt ctgatcaact aaaggaaaag ataaatgacc tggagacaca
     9241 actcagaaag ttggagctag aaaagcaaca ttcgaaggag gaagttaaaa agctgaaaaa
     9301 agaactggaa aattttgatc cttcattttt tgaagaaatt gaagacctga agtataatta
     9361 taaggaagaa gtgaaaaaga atatcctatt agaagagaag ctaaaaaaac tgtcggaaca
     9421 gtttggattt gaactgccta gtcctcttgc tgcttctgaa cactcggaag atggagaaag
     9481 tcctcatagt ttccctattt attagtgccc tcgtgtaact gcaggtttaa aactatttta
     9541 tgagtaaaaa aagtttcagc tggaaatcaa gcagtctctg tgttataagg gcgaattcca
     9601 gcacactggc ggccgttact agtggatcca ccggatctag ataactgatc ataatcagcc
     9661 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     9721 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     9781 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9841 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt
     9901 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     9961 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
    10021 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
    10081 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
    10141 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
    10201 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
    10261 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
    10321 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
    10381 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
    10441 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
    10501 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    10561 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
    10621 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
    10681 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
    10741 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    10801 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
    10861 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
    10921 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
    10981 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
    11041 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
    11101 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
    11161 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
    11221 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
    11281 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
    11341 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
    11401 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
    11461 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
    11521 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
    11581 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
    11641 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
    11701 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
    11761 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    11821 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
    11881 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
    11941 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
    12001 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
    12061 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
    12121 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    12181 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    12241 tagaaaagat caaaggatct tc
//
