LOCUS       sequence-322024-        9112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the regulated expression of the PhiC31 phage integrase gene 
            driven by a synthetic promoter consisting of the LexA operator fused
            to the minimal 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB...
FEATURES             Location/Qualifiers
     source          1..9112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        284..330
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             405..2219
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             2220..2240
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2267..2519
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2554..2572)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2756..2780
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4081..4710
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5139..6212
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6278..6472
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6708..6730)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6816..6956
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6871..6890
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6971..6988)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7142..7730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7222..7241)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7818..8633)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7818..8633)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8541..8560
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9066..9090
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagagcttg ggctgcaggt cgaggctaaa aaactaatcg cattatcatc ccctcgacgt
       61 actgtacata taaccactgg ttttatatac agcagtactg tacatataac cactggtttt
      121 atatacagca gtcgacgtac tgtacatata accactggtt ttatatacag cagtactgta
      181 catataacca ctggttttat atacagcagt cgaggtaaga ttagatatgg atatgtatat
      241 ggatatgtat atggtggtaa tgccatgtaa tatgctcccc ttcgcaagac ccttcctcta
      301 tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa ttaccacaac
      361 aaaacaaaca acaaacaaca ttacaattta ctattctagt cgaaatggac acgtatgccg
      421 gcgcttatga tagacaatca agagaaagag agaactcttc tgccgcctct ccagctacac
      481 agagatctgc caacgaggat aaagctgctg atttacaaag agaggtagag agagatgggg
      541 gtaggtttag atttgtgggt catttctctg aggctcctgg tacatcagcc tttggtactg
      601 ctgaacggcc tgaatttgaa agaatattaa atgagtgtcg ggccggtcga ctaaatatga
      661 taatagttta tgatgtatct aggttcagta gactcaaagt tatggatgca attccgatcg
      721 tgagcgagct acttgcactc ggtgttacta tcgtaagtac acaagaaggt gtattcaggc
      781 agggtaatgt gatggacctt atacacctta ttatgcgact cgacgctagc cataaagagt
      841 catcactgaa gagtgctaaa atcttggaca ccaagaacct gcaaagggag cttggaggct
      901 atgtaggagg aaaagctccg tatggtttcg aactcgtgag tgagacaaaa gagattacca
      961 gaaatggtag gatggtgaac gtggttatta ataagctagc tcatagcaca acccctctca
     1021 cagggccttt tgagttcgaa cctgacgtta ttagatggtg gtggagagag atcaaaactc
     1081 acaaacatct tccatttaag ccaggtagcc aggctgctat ccaccctgga tctatcactg
     1141 ggctttgtaa aaggatggat gctgacgcag tgccaactcg aggggaaaca atcggtaaga
     1201 agactgcatc ttccgcttgg gatcccgcta cagttatgag aatccttcgg gatccgcgta
     1261 ttgctggatt cgccgcagaa gtcatttata agaaaaaacc tgatggtact cctactacaa
     1321 aaatcgaagg ttataggatc caaagggatc ctattacttt gcgtccagtt gaattagact
     1381 gtggaccgat catcgaacct gccgagtggt acgagttgca ggcctggctt gatggtagag
     1441 ggcgtggaaa gggtttatct cggggacagg caattttgtc agcaatggat aagctttatt
     1501 gtgagtgtgg ggctgttatg acatcaaaac gtggagaaga gtcaatcaag gattcttaca
     1561 ggtgcagacg tcgtaaagtg gtggaccctt ctgctccagg acagcatgag ggtacatgca
     1621 acgtttctat ggctgctctt gataaatttg tggccgaacg tattttcaac aagattagac
     1681 acgctgaagg ggatgaggaa actttagctc ttctttggga ggctgcccgg cgttttggta
     1741 agctaacaga ggcaccagaa aaaagtggcg aaagagctaa tttggtagcc gaaagagcag
     1801 acgccttgaa tgcattggaa gaactttacg aagatagggc tgccggagca tatgatggac
     1861 cagttggtcg gaagcatttc aggaagcaac aagctgcact aactttgaga cagcaaggcg
     1921 cagaggagcg actcgcagag cttgaggccg ccgaggcccc aaagcttcca ctcgatcaat
     1981 ggtttcctga ggatgccgac gctgatccta caggtccaaa gagctggtgg ggaagagcta
     2041 gcgtagacga taagagagtg tttgtgggtc tatttgttga caagattgta gttactaagt
     2101 ccactacggg tcgaggacag ggcactccca tcgaaaagag agccagtatt acctgggcaa
     2161 agcctccaac cgatgatgat gaagacgatg cacaagatgg taccgaggat gtggcagcac
     2221 caaaaaaaaa gaggaaggtg taagcttgga atggatcttc gatcccgatc gttcaaacat
     2281 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgacga ttatcatata
     2341 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     2401 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     2461 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     2521 ggaattgcca agctaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     2581 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     2641 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2701 aataaccctg ataaatgctt caataatggg accgactcgc gcttgagacg aagcttgaca
     2761 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     2821 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
     2881 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
     2941 gacgttcagt gcagccgtca tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     3001 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     3061 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     3121 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     3181 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     3241 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     3301 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     3361 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     3421 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     3481 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     3541 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     3601 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     3661 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     3721 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     3781 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     3841 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg tgcggctgca
     3901 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     3961 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     4021 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     4081 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     4141 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     4201 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     4261 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     4321 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     4381 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     4441 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     4501 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     4561 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     4621 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     4681 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     4741 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     4801 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     4861 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     4921 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     4981 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     5041 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc
     5101 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     5161 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     5221 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     5281 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     5341 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     5401 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     5461 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     5521 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     5581 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     5641 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     5701 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     5761 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     5821 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     5881 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     5941 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     6001 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     6061 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     6121 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     6181 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     6241 ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt ggatagcacg tacattggga
     6301 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     6361 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     6421 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     6481 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     6541 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
     6601 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     6661 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     6721 cagctcccgg tgacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     6781 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     6841 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     6901 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     6961 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7141 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7321 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7561 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7741 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7801 tcatgcattc taggtgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     7861 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     7921 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     7981 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     8041 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag
     8101 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     8161 ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc gatcgctgtt aaaaggacaa
     8221 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     8281 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttccctgg gatcgcagtg
     8341 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     8401 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     8461 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg gtagattgtc
     8521 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     8581 ttggaattta atcgcggcct tgagcaagac gtttcccgtt gaatatggct cataacagaa
     8641 cttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa
     8701 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc
     8761 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa
     8821 accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg
     8881 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt
     8941 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat
     9001 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg
     9061 gctggtggca ggatatattg tggtgtaaac ataacaagct tcgtctcagt ca
//
