LOCUS       sequence-322029-       11373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for the expression of the LbCas12a in plant 
            systems driven by the CaMV35s promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Bernabe-Orts JM, Casas-Rodrigo I, Minguet EG, Landolfi V, 
            Garcia-Carpintero V, Gianoglio S, Vazquez-Vilar M, Granell A, Orzaez
            D
  TITLE     Assessment of Cas12a-mediated gene editing efficiency in plants.
  JOURNAL   Plant Biotechnol J. 2019 Apr 5. doi: 10.1111/pbi.13113.
  PUBMED    30950179
REFERENCE   2  (bases 1 to 11373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2019 Apr 5. doi: 10.1111/pbi.13113."
COMMENT     Sequence Label: pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)
FEATURES             Location/Qualifiers
     source          1..11373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        616..960
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     904..924
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1034..4717
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4718..4765
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    5022..5046
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6347..6976
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7405..8478
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8544..8738
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8974..8996)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9082..9222
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9137..9156
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9237..9254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9408..9996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9488..9507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10084..10899)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10084..10899)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10807..10826
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    11332..11356
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcccctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat tacaatttac
     1021 tattctagtc gaaatgagca agctggagaa gtttacaaac tgctactccc tgtctaagac
     1081 cctgaggttc aaggccatcc ctgtgggcaa gacccaggag aacatcgaca ataagcggct
     1141 gctggtggag gacgagaaga gagccgagga ttataagggc gtgaagaagc tgctggatcg
     1201 ctactatctg tcttttatca acgacgtgct gcacagcatc aagctgaaga atctgaacaa
     1261 ttacatcagc ctgttccgga agaaaaccag aaccgagaag gagaataagg agctggagaa
     1321 cctggagatc aatctgcgga aggagatcgc caaggccttc aagggcaacg agggctacaa
     1381 gtccctgttt aagaaggata tcatcgagac aatcctgcca gagttcctgg acgataagga
     1441 cgagatcgcc ctggtgaaca gcttcaatgg ctttaccaca gccttcaccg gcttctttga
     1501 taacagagag aatatgtttt ccgaggaggc caagagcaca tccatcgcct tcaggtgtat
     1561 caacgagaat ctgacccgct acatctctaa tatggacatc ttcgagaagg tggacgccat
     1621 ctttgataag cacgaggtgc aggagatcaa ggagaagatc ctgaacagcg actatgatgt
     1681 ggaggatttc tttgagggcg agttctttaa ctttgtgctg acacaggagg gcatcgacgt
     1741 gtataacgcc atcatcggcg gcttcgtgac cgagagcggc gagaagatca agggcctgaa
     1801 cgagtacatc aacctgtata atcagaaaac caagcagaag ctgcctaagt ttaagccact
     1861 gtataagcag gtgctgagcg atcgggagtc tctgagcttc tacggcgagg gctatacatc
     1921 cgatgaggag gtgctggagg tgtttagaaa caccctgaac aagaacagcg agatcttcag
     1981 ctccatcaag aagctggaga agctgttcaa gaattttgac gagtactcta gcgccggcat
     2041 ctttgtgaag aacggccccg ccatcagcac aatctccaag gatatcttcg gcgagtggaa
     2101 cgtgatccgg gacaagtgga atgccgagta tgacgatatc cacctgaaga agaaggccgt
     2161 ggtgaccgag aagtacgagg acgatcggag aaagtccttc aagaagatcg gctccttttc
     2221 tctggagcag ctgcaggagt acgccgacgc cgatctgtct gtggtggaga agctgaagga
     2281 gatcatcatc cagaaggtgg atgagatcta caaggtgtat ggctcctctg agaagctgtt
     2341 cgacgccgat tttgtgctgg agaagagcct gaagaagaac gacgccgtgg tggccatcat
     2401 gaaggacctg ctggattctg tgaagagctt cgagaattac atcaaggcct tctttggcga
     2461 gggcaaggag acaaacaggg acgagtcctt ctatggcgat tttgtgctgg cctacgacat
     2521 cctgctgaag gtggaccaca tctacgatgc catccgcaat tatgtgaccc agaagcccta
     2581 ctctaaggat aagttcaagc tgtattttca gaaccctcag ttcatgggcg gctgggacaa
     2641 ggataaggag acagactatc gggccaccat cctgagatac ggctccaagt actatctggc
     2701 catcatggat aagaagtacg ccaagtgcct gcagaagatc gacaaggacg atgtgaacgg
     2761 caattacgag aagatcaact ataagctgct gcccggccct aataagatgc tgccaaaggt
     2821 gttcttttct aagaagtgga tggcctacta taaccccagc gaggacatcc agaagatcta
     2881 caagaatggc acattcaaga agggcgatat gtttaacctg aatgactgtc acaagctgat
     2941 cgacttcttt aaggatagca tctcccggta tccaaagtgg tccaatgcct acgatttcaa
     3001 cttttctgag acagagaagt ataaggacat cgccggcttt tacagagagg tggaggagca
     3061 gggctataag gtgagcttcg agtctgccag caagaaggag gtggataagc tggtggagga
     3121 gggcaagctg tatatgttcc agatctataa caaggacttt tccgataagt ctcacggcac
     3181 acccaatctg cacaccatgt acttcaagct gctgtttgac gagaacaatc acggacagat
     3241 caggctgagc ggaggagcag agctgttcat gaggcgcgcc tccctgaaga aggaggagct
     3301 ggtggtgcac ccagccaact cccctatcgc caacaagaat ccagataatc ccaagaaaac
     3361 cacaaccctg tcctacgacg tgtataagga taagaggttt tctgaggacc agtacgagct
     3421 gcacatccca atcgccatca ataagtgccc caagaacatc ttcaagatca atacagaggt
     3481 gcgcgtgctg ctgaagcacg acgataaccc ctatgtgatc ggcatcgata ggggcgagcg
     3541 caatctgctg tatatcgtgg tggtggacgg caagggcaac atcgtggagc agtattccct
     3601 gaacgagatc atcaacaact tcaacggcat caggatcaag acagattacc actctctgct
     3661 ggacaagaag gagaaggaga ggttcgaggc ccgccagaac tggacctcca tcgagaatat
     3721 caaggagctg aaggccggct atatctctca ggtggtgcac aagatctgcg agctggtgga
     3781 gaagtacgat gccgtgatcg ccctggagga cctgaactct ggctttaaga atagccgcgt
     3841 gaaggtggag aagcaggtgt atcagaagtt cgagaagatg ctgatcgata agctgaacta
     3901 catggtggac aagaagtcta atccttgtgc aacaggcggc gccctgaagg gctatcagat
     3961 caccaataag ttcgagagct ttaagtccat gtctacccag aacggcttca tcttttacat
     4021 ccctgcctgg ctgacatcca agatcgatcc atctaccggc tttgtgaacc tgctgaaaac
     4081 caagtatacc agcatcgccg attccaagaa gttcatcagc tcctttgaca ggatcatgta
     4141 cgtgcccgag gaggatctgt tcgagtttgc cctggactat aagaacttct ctcgcacaga
     4201 cgccgattac atcaagaagt ggaagctgta ctcctacggc aaccggatca gaatcttccg
     4261 gaatcctaag aagaacaacg tgttcgactg ggaggaggtg tgcctgacca gcgcctataa
     4321 ggagctgttc aacaagtacg gcatcaatta tcagcagggc gatatcagag ccctgctgtg
     4381 cgagcagtcc gacaaggcct tctactctag ctttatggcc ctgatgagcc tgatgctgca
     4441 gatgcggaac agcatcacag gccgcaccga cgtggatttt ctgatcagcc ctgtgaagaa
     4501 ctccgacggc atcttctacg atagccggaa ctatgaggcc caggagaatg ccatcctgcc
     4561 aaagaacgcc gacgccaatg gcgcctataa catcgccaga aaggtgctgt gggccatcgg
     4621 ccagttcaag aaggccgagg acgagaagct ggataaggtg aagatcgcca tctctaacaa
     4681 ggagtggctg gagtacgccc agaccagcgt gaagcacaaa aggccggcgg ccacgaaaaa
     4741 ggccggccag gcaaaaaaga aaaagtagtg agcttcggcc atgctagagt ccgcaaaaat
     4801 caccagtctc tctctacaaa tctatctctc tctatttttc tccagaataa tgtgtgagta
     4861 gttcccagat aagggaatta gggttcttat agggtttcgc tcatgtgttg agcatataag
     4921 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
     4981 aaaaccaaaa tccagtgacc tcgctgtcat gagacgaatt ctgacaggat atattggcgg
     5041 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     5101 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
     5161 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
     5221 ccgtcatctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
     5281 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
     5341 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
     5401 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
     5461 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
     5521 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
     5581 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
     5641 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
     5701 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
     5761 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
     5821 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
     5881 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
     5941 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
     6001 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
     6061 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
     6121 ggtacgtgtt cgagccgccc gcgcacctct caaccgtgcg gctgcatgaa atcctggccg
     6181 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     6241 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     6301 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     6361 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     6421 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     6481 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     6541 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     6601 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     6661 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     6721 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     6781 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     6841 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     6901 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     6961 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     7021 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     7081 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     7141 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     7201 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     7261 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     7321 aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg
     7381 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     7441 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     7501 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     7561 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     7621 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     7681 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     7741 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     7801 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     7861 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     7921 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     7981 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     8041 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     8101 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     8161 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     8221 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     8281 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     8341 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     8401 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     8461 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     8521 aaaggtcgaa aaggactctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     8581 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     8641 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     8701 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     8761 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     8821 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     8881 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     8941 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggtgac
     9001 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     9061 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     9121 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     9181 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     9241 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     9301 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9361 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9421 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9481 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9541 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9601 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9661 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9721 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9781 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9841 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9901 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9961 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    10021 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg
    10081 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
    10141 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
    10201 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
    10261 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
    10321 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
    10381 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
    10441 ttgcgcctga gcgagtcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
    10501 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
    10561 atattcttct aatacctgga atgctgtttt ccctgggatc gcagtggtga gtaaccatgc
    10621 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
    10681 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
    10741 aaacaactct ggcgcatcgg gcttcccata caatcggtag attgtcgcac ctgattgccc
    10801 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
    10861 cggccttgag caagacgttt cccgttgaat atggctcata acagaactta ttatttcctt
    10921 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    10981 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    11041 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
    11101 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
    11161 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
    11221 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
    11281 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
    11341 atattgtggt gtaaacataa cgaattcgtc tca
//
