LOCUS       sequence-322039-       11610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for the expression of the AsCas12a in plant 
            systems driven by the CaMV35s promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Bernabe-Orts JM, Casas-Rodrigo I, Minguet EG, Landolfi V, 
            Garcia-Carpintero V, Gianoglio S, Vazquez-Vilar M, Granell A, Orzaez
            D
  TITLE     Assessment of Cas12a-mediated gene editing efficiency in plants.
  JOURNAL   Plant Biotechnol J. 2019 Apr 5. doi: 10.1111/pbi.13113.
  PUBMED    30950179
REFERENCE   2  (bases 1 to 11610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2019 Apr 5. doi: 10.1111/pbi.13113."
COMMENT     Sequence Label: pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)
FEATURES             Location/Qualifiers
     source          1..11610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        616..960
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     904..924
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1034..4954
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      4674..4683
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4955..5002
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    5259..5283
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6584..7213
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7642..8715
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8781..8975
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9211..9233)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9319..9459
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9374..9393
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9474..9491)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9645..10233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9725..9744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10321..11136)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10321..11136)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     11044..11063
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    11569..11593
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcccctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat tacaatttac
     1021 tattctagtc gaaatgacac agttcgaggg ctttaccaac ctgtatcagg tgagcaagac
     1081 actgcggttt gagctgatcc cacagggcaa gaccctgaag cacatccagg agcagggctt
     1141 catcgaggag gacaaggccc gcaatgatca ctacaaggag ctgaagccca tcatcgatcg
     1201 gatctacaag acctatgccg accagtgcct gcagctggtg cagctggatt gggagaacct
     1261 gagcgccgcc atcgactcct atagaaagga gaaaaccgag gagacaagga acgccctgat
     1321 cgaggagcag gccacatatc gcaatgccat ccacgactac ttcatcggcc ggacagacaa
     1381 cctgaccgat gccatcaata agagacacgc cgagatctac aagggcctgt tcaaggccga
     1441 gctgtttaat ggcaaggtgc tgaagcagct gggcaccgtg accacaaccg agcacgagaa
     1501 cgccctgctg cggagcttcg acaagtttac aacctacttc tccggctttt atgagaacag
     1561 gaagaacgtg ttcagcgccg aggatatcag cacagccatc ccacaccgca tcgtgcagga
     1621 caacttcccc aagtttaagg agaattgtca catcttcaca cgcctgatca ccgccgtgcc
     1681 cagcctgcgg gagcactttg agaacgtgaa gaaggccatc ggcatcttcg tgagcacctc
     1741 catcgaggag gtgttttcct tcccttttta taaccagctg ctgacacaga cccagatcga
     1801 cctgtataac cagctgctgg gaggaatctc tcgggaggca ggcaccgaga agatcaaggg
     1861 cctgaacgag gtgctgaatc tggccatcca gaagaatgat gagacagccc acatcatcgc
     1921 ctccctgcca cacagattca tccccctgtt taagcagatc ctgtccgata ggaacaccct
     1981 gtctttcatc ctggaggagt ttaagagcga cgaggaagtg atccagtcct tctgcaagta
     2041 caagacactg ctgagaaacg agaacgtgct ggagacagcc gaggccctgt ttaacgagct
     2101 gaacagcatc gacctgacac acatcttcat cagccacaag aagctggaga caatcagcag
     2161 cgccctgtgc gaccactggg atacactgag gaatgccctg tatgagcgga gaatctccga
     2221 gctgacaggc aagatcacca agtctgccaa ggagaaggtg cagcgcagcc tgaagcacga
     2281 ggatatcaac ctgcaggaga tcatctctgc cgcaggcaag gagctgagcg aggccttcaa
     2341 gcagaaaacc agcgagatcc tgtcccacgc acacgccgcc ctggatcagc cactgcctac
     2401 aaccctgaag aagcaggagg agaaggagat cctgaagtct cagctggaca gcctgctggg
     2461 cctgtaccac ctgctggact ggtttgccgt ggatgagtcc aacgaggtgg accccgagtt
     2521 ctctgcccgg ctgaccggca tcaagctgga gatggagcct tctctgagct tctacaacaa
     2581 ggccagaaat tatgccacca agaagcccta ctccgtggag aagttcaagc tgaactttca
     2641 gatgcctaca ctggcctctg gctgggacgt gaataaggag aagaacaatg gcgccatcct
     2701 gtttgtgaag aacggcctgt actatctggg catcatgcca aagcagaagg gcaggtataa
     2761 ggccctgagc ttcgagccca cagagaaaac cagcgagggc tttgataaga tgtactatga
     2821 ctacttccct gatgccgcca agatgatccc aaagtgcagc acccagctga aggccgtgac
     2881 agcccacttt cagacccaca caacccccat cctgctgtcc aacaatttca tcgagcctct
     2941 ggagatcaca aaggagatct acgacctgaa caatcctgag aaggagccaa agaagtttca
     3001 gacagcctac gccaagaaaa ccggcgacca gaagggctac agagaggccc tgtgcaagtg
     3061 gatcgacttc acaagggatt ttctgtccaa gtataccaag acaacctcta tcgatctgtc
     3121 tagcctgcgg ccatcctctc agtataagga cctgggcgag tactatgccg agctgaatcc
     3181 cctgctgtac cacatcagct tccagagaat cgccgagaag gagatcatgg atgccgtgga
     3241 gacaggcaag ctgtacctgt tccagatcta taacaaggac tttgccaagg gccaccacgg
     3301 caagcctaat ctgcacacac tgtattggac cggcctgttt tctccagaga acctggccaa
     3361 gacaagcatc aagctgaatg gccaggccga gctgttctac cgccctaagt ccaggatgaa
     3421 gaggatggca caccggctgg gagagaagat gctgaacaag aagctgaagg atcagaaaac
     3481 cccaatcccc gacaccctgt accaggagct gtacgactat gtgaatcaca gactgtccca
     3541 cgacctgtct gatgaggcca gggccctgct gcccaacgtg atcaccaagg aggtgtctca
     3601 cgagatcatc aaggataggc gctttaccag cgacaagttc tttttccacg tgcctatcac
     3661 actgaactat caggccgcca attccccatc taagttcaac cagagggtga atgcctacct
     3721 gaaggagcac cccgagacac ctatcatcgg catcgatcgg ggcgagagaa acctgatcta
     3781 tatcacagtg atcgactcca ccggcaagat cctggagcag cggagcctga acaccatcca
     3841 gcagtttgat taccagaaga agctggacaa cagggagaag gagagggtgg cagcaaggca
     3901 ggcctggtct gtggtgggca caatcaagga tctgaagcag ggctatctga gccaggtcat
     3961 ccacgagatc gtggacctga tgatccacta ccaggccgtg gtggtgctgg agaacctgaa
     4021 tttcggcttt aagagcaaga ggaccggcat cgccgagaag gccgtgtacc agcagttcga
     4081 gaagatgctg atcgataagc tgaattgcct ggtgctgaag gactatccag cagagaaagt
     4141 gggaggcgtg ctgaacccat accagctgac agaccagttc acctcctttg ccaagatggg
     4201 cacccagtct ggcttcctgt tttacgtgcc tgccccatat acatctaaga tcgatcccct
     4261 gaccggcttc gtggacccct tcgtgtggaa aaccatcaag aatcacgaga gccgcaagca
     4321 cttcctggag ggcttcgact ttctgcacta cgacgtgaaa accggcgact tcatcctgca
     4381 ctttaagatg aacagaaatc tgtccttcca gaggggcctg cccggcttta tgcctgcatg
     4441 ggatatcgtg ttcgagaaga acgagacaca gtttgacgcc aagggcaccc ctttcatcgc
     4501 cggcaagaga atcgtgccag tgatcgagaa tcacagattc accggcagat accgggacct
     4561 gtatcctgcc aacgagctga tcgccctgct ggaggagaag ggcatcgtgt tcagggatgg
     4621 ctccaacatc ctgccaaagc tgctggagaa tgacgattct cacgccatcg acaccatggt
     4681 ggccctgatc cgcagcgtgc tgcagatgcg gaactccaat gccgccacag gcgaggacta
     4741 tatcaacagc cccgtgcgcg atctgaatgg cgtgtgcttc gactcccggt ttcagaaccc
     4801 agagtggccc atggacgccg atgccaatgg cgcctaccac atcgccctga agggccagct
     4861 gctgctgaat cacctgaagg agagcaagga tctgaagctg cagaacggca tctccaatca
     4921 ggactggctg gcctacatcc aggagctgcg caacaaaagg ccggcggcca cgaaaaaggc
     4981 cggccaggca aaaaagaaaa agtagtgagc ttcggccatg ctagagtccg caaaaatcac
     5041 cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt
     5101 cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
     5161 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
     5221 accaaaatcc agtgacctcg ctgtcatgag acgaattctg acaggatata ttggcgggta
     5281 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     5341 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
     5401 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
     5461 tcatctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
     5521 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
     5581 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
     5641 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
     5701 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
     5761 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
     5821 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
     5881 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
     5941 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     6001 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     6061 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     6121 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     6181 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     6241 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     6301 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     6361 acgtgttcga gccgcccgcg cacctctcaa ccgtgcggct gcatgaaatc ctggccggtt
     6421 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     6481 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     6541 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     6601 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     6661 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     6721 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     6781 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     6841 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     6901 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     6961 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     7021 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     7081 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     7141 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     7201 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     7261 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     7321 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     7381 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     7441 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     7501 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     7561 gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac
     7621 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
     7681 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
     7741 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
     7801 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
     7861 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
     7921 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
     7981 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
     8041 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
     8101 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
     8161 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
     8221 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
     8281 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
     8341 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
     8401 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
     8461 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
     8521 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
     8581 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
     8641 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
     8701 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
     8761 ggtcgaaaag gactctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
     8821 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
     8881 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
     8941 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
     9001 gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt
     9061 cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca
     9121 gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg
     9181 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggtgacggt
     9241 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     9301 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
     9361 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     9421 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
     9481 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9541 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9601 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9661 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9721 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9781 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9841 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9901 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9961 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    10021 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    10081 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    10141 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10201 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10261 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtga
    10321 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
    10381 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
    10441 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
    10501 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
    10561 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
    10621 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
    10681 cgcctgagcg agtcgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
    10741 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
    10801 ttcttctaat acctggaatg ctgttttccc tgggatcgca gtggtgagta accatgcatc
    10861 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
    10921 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
    10981 caactctggc gcatcgggct tcccatacaa tcggtagatt gtcgcacctg attgcccgac
    11041 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
    11101 ccttgagcaa gacgtttccc gttgaatatg gctcataaca gaacttatta tttccttcct
    11161 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
    11221 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
    11281 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
    11341 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
    11401 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
    11461 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
    11521 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
    11581 ttgtggtgta aacataacga attcgtctca
//
