LOCUS       sequence-322048-       10762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10762)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 10762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-PBRM1
FEATURES             Location/Qualifiers
     source          1..10762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     817..841
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(945..947,948..950,951..1661)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(990..1011)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1251..1270)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1598..1619
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1677..1701
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6589..6613)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6621..6662
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6702..6721)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6746..6794
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6996..7424
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7083..7102)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7293..7314
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7433..7453)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7438..7767
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7618..7753
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7680..7699
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7815..7862
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7881..8279
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8437..8558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8474..8493)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8528..8547
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8607..8623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8607..8623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8620..8642)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8631..8647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8655..8661,8662..8679,8680..8685))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8700..8721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8838..8855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9009..9597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9089..9108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9768..10559,10560..10628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10391..10410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10629..10733)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
      841 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      901 cctccggact ctagaggatc cctaccggtg atatcctcga gaccatggtg agcaagggcg
      961 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1021 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1081 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttca
     1141 cctacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     1201 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1261 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1321 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1381 acaacagcca caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact
     1441 tcaagacccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1501 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     1561 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1621 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggctcgagc ccatcaacaa
     1681 gtttgtacaa aaaagcaggc ttcgaaggag atagaaccaa ttctctaagg aaatacttaa
     1741 ccatggtcga ctggatccgg tacctcatgg gttccaagag aagaagagct acctcccctt
     1801 ccagcagtgt cagcggggac tttgatgatg ggcaccattc tgtgtcaaca ccaggcccaa
     1861 gcaggaaaag gaggagactt tccaatcttc caactgtaga tcctattgcc gtgtgccatg
     1921 aactctataa taccatccga gactataagg atgaacaggg cagacttctc tgtgagctct
     1981 tcattagggc accaaagcga agaaatcaac cagactatta tgaagtggtt tctcagccca
     2041 ttgacttgat gaaaatccaa cagaaactaa aaatggaaga gtatgatgat gttaatttgc
     2101 tgactgctga cttccagctt ctttttaaca atgcaaagtc ctattataag ccagattctc
     2161 ctgaatataa agccgcttgc aaactctggg atttgtacct tcgaacaaga aatgagtttg
     2221 ttcagaaagg agaagcagat gacgaagatg atgatgaaga tgggcaagac aatcagggca
     2281 cagtgactga aggatcttct ccagcttact tgaaggagat cctggagcag cttcttgaag
     2341 ccatagttgt agctacaaat ccatcaggac gtctcattag cgaacttttt cagaaactgc
     2401 cttctaaagt gcaatatcca gattattatg caataattaa ggagcctata gatctcaaga
     2461 ccattgccca gaggatacag aatggaagct acaaaagtat tcatgcaatg gccaaagata
     2521 tagatctcct cgcaaaaaat gccaaaactt ataatgagcc tggctctcaa gtattcaagg
     2581 atgcaaattc aattaaaaaa atattttata tgaaaaaggc tgaaattgaa catcatgaaa
     2641 tggctaagtc aagtcttcga atgaggactc catccaactt ggctgcagcc agactgacag
     2701 gtccttcaca cagtaaaggc agccttggtg aagagagaaa tcccactagc aagtattacc
     2761 gtaataaaag agcagtacaa ggaggtcgtt tatcagcaat tacaatggca cttcaatatg
     2821 gctcagaaag tgaagaagat gctgctttag ctgctgcacg ctatgaagag ggagagtcag
     2881 aagcagaaag catcacttcc tttatggatg tttcaaatcc tttttatcag ctttatgaca
     2941 cagttaggag ttgtcggaat aaccaagggc agctaatagc tgaacctttt taccatttgc
     3001 cttcaaagaa aaaataccct gattattacc agcaaattaa aatgcccata tcactacaac
     3061 agatccgaac aaaactgaag aatcaagaat atgaaacttt agatcatttg gagtgtgatc
     3121 tgaatttaat gtttgaaaat gccaaacgct ataatgtgcc caattcagcc atctacaagc
     3181 gagttctaaa attgcagcaa gttatgcagg caaagaagaa agagcttgcc aggagagacg
     3241 atatcgagga cggagacagc atgatctctt cagccacctc tgatactggt agtgccaaaa
     3301 gaaaaaggaa cactcatgac agtgagatgt tgggtctcag gaggctatcc agtaaaaaga
     3361 acataagaaa gcagcgaatg aaaatcttat tcaatgttgt tcttgaagct cgagagccag
     3421 gttcaggcag aagactttgt gacctattta tggttaaacc atccaaaaag gactatcctg
     3481 attattataa aatcatcttg gagccaatgg acttgaaaat aattgagcat aacatccgca
     3541 atgacaaata tgctggtgaa gagggaatga tagaagacat gaagctgatg ttccggaatg
     3601 ccaggcacta taatgaggag ggctcccagg tttataatga tgcacatatc ctggagaagt
     3661 tactcaagga gaaaaggaaa gagctgggcc cactgcctga tgatgatgac atggcttctc
     3721 ccaaactcaa gctgagtagg aagagtggca tttctcctaa aaaatcaaaa tacatgactc
     3781 caatgcagca gaaactaaat gaggtctatg aagctgtaaa gaactatact gataagaggg
     3841 gtcgccgcct cagtgccata tttctgaggc ttccctctag atctgagttg cctgactact
     3901 atctgactat taaaaagccc atggacatgg aaaaaattcg aagtcacatg atggccaaca
     3961 agtaccaaga tattgactct atggttgagg actttgtcat gatgtttaat aatgcctgta
     4021 catacaatga gccggagtct ttgatctaca aagatgctct tgttctacac aaagtcctgc
     4081 ttgaaacacg cagagacctg gagggagatg aggactctca tgtcccaaat gtgactttgc
     4141 tgattcaaga gcttatccac aatctttttg tgtcagtcat gagtcatcag gatgatgagg
     4201 gaagatgcta cagcgattct ttagcagaaa ttcctgctgt ggatcccaac tttcctaaca
     4261 aaccacccct tacatttgac ataattagga agaatgttga aaataatcgc taccgtcggc
     4321 ttgatttatt tcaagagcat atgtttgaag tattggaacg agcaagaagg atgaatcgga
     4381 cagattcaga aatatatgaa gatgcagtag aacttcagca gttttttatt aaaattcgtg
     4441 atgaactctg caaaaatgga gagattcttc tttcaccggc actcagctat accacgaaac
     4501 atttgcataa tgatgtggag aaagagagaa aggaaaaatt gccaaaagaa atagaggaag
     4561 ataaactaaa acgagaagaa gaaaaaagag aagctgaaaa gagtgaagat tcctctggtg
     4621 ctgcaggcct ctcaggctta catcgcacat acagccagga ctgtagcttt aaaaacagca
     4681 tgtaccatgt tggagattac gtctatgtgg aacctgcaga ggccaaccta caaccacata
     4741 tcgtctgtat tgaaagactg tgggaggatt cagctggtga aaaatggttg tatggctgtt
     4801 ggttttaccg accaaatgaa acattccacc tggctacacg aaaatttcta gaaaaagaag
     4861 tttttaagag tgactattac aacaaagttc cagttagtaa aattctaggc aagtgtgtgg
     4921 tcatgtttgt caaggaatac tttaagttat gcccagaaaa cttccgagat gaggatgttt
     4981 ttgtctgtga atcacggtat tctgccaaaa ccaaatcttt taagaaaatt aaactgtgga
     5041 ccatgcccat cagctcagtc aggtttgtcc ctcgggatgt gcctctgcct gtggttcgcg
     5101 tggcctctgt atttgcaaat gcagataaag gtgatgatga gaagaataca gacaactcag
     5161 aggacagtcg agctgaagac aattttaact tggaaaagga aaaagaagat gtccctgtgg
     5221 aaatgtccaa tggtgaacca ggttgccact actttgagca gctccattac aatgacatgt
     5281 ggctgaaggt tggcgactgt gtcttcatca agtcccatgg cctggtgcgt cctcgtgtgg
     5341 gcagaattga aaaagtatgg gttcgagatg gagctgcata tttttatggc cccatcttca
     5401 ttcacccaga agaaacagag catgagccca caaaaatgtt ctacaaaaaa gaagtatttc
     5461 tgagtaatct ggaagaaacc tgccccatga catgtattct cggaaagtgt gctgtgttgt
     5521 cattcaagga cttcctctcc tgcaggccaa ctgaaatacc agaaaatgac attctgcttt
     5581 gtgagagccg ctacaatgag agcgacaagc agatgaagaa attcaaagga ttgaagaggt
     5641 tttcactctc tgctaaagtg gtagatgatg aaatttacta cttcagaaaa ccaattgttc
     5701 ctcagaagga gccatcacct ttgctggaaa agaagatcca gttgctagaa gctaaatttg
     5761 ccgagttaga aggtggagat gatgatattg aagagatggg agaagaagat agtgaggtca
     5821 ttgaacctcc ttctctacct cagcttcaga cccccctggc cagtgagctg gacctcatgc
     5881 cctacacacc cccacagtct accccaaagt ctgccaaagg cagtgcaaag aaggaaggct
     5941 ccaaacggaa aatcaacatg agtggctaca tcctgttcag cagtgagatg agggctgtga
     6001 ttaaggccca acacccagac tactctttcg gggagctcag ccgcctggtg gggacagaat
     6061 ggagaaatct tgagacagcc aagaaagcag aatatgaagg tgtgatgaac caaggagtgg
     6121 cccctatggt agggactcca gcaccaggtg gaagtccata tggacaacag gtgggagttt
     6181 tggggcctcc agggcagcag gcaccacctc catatcccgg cccacatcca gctggacccc
     6241 ctgtcataca gcagccaaca acacccatgt ttgtagctcc cccaccaaag acccagcggc
     6301 ttcttcactc agaggcctac ctgaaataca ttgaaggact cagtgcggag tccaacagca
     6361 ttagcaagtg ggatcagaca ctggcagctc gaagacgcga cgtccatttg tcgaaagaac
     6421 aggagagccg cctaccctct cactggctga aaagcaaagg ggcccacacc accatggcag
     6481 atgccctctg gcgccttcga gatttgatgc tccgggacac cctcaacatt cgccaagcat
     6541 acaacctaga aaatgttttg aattcgcggc cgcactcgag atatctagac ccagctttct
     6601 tgtacaaagt ggttgataac gggaagccta tccctaaccc tctcctcggt ctcgattcta
     6661 cgcgtaccgg ttagtaatga gtttaaacgg gggaggctaa ctgaaacacg gaaggagaca
     6721 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt
     6781 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     6841 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     6901 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     6961 agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     7021 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     7081 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     7141 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     7201 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     7261 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7321 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     7381 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     7441 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7501 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7561 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7621 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7681 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     7741 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     7801 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     7861 acaaggtgag gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg
     7921 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     7981 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     8041 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     8101 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     8161 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     8221 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag
     8281 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
     8341 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     8401 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     8461 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8521 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     8581 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8641 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8701 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8761 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8821 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8881 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8941 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9001 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9061 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9121 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9181 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9241 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9301 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9361 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9421 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9481 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9541 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9601 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9661 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9721 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9781 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9841 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9901 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9961 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10021 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10081 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10141 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10201 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10261 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10321 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10381 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10441 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10501 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10561 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10621 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10681 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10741 ccccgaaaag tgccacctga cg
//
