LOCUS       sequence-32205-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Lian X, Hsiao C, Wilson G, Zhu K, Hazeltine LB, Azarin SM, Raval KK,
            Zhang J, Kamp TJ, Palecek SP
  TITLE     Robust cardiomyocyte differentiation from human pluripotent stem 
            cells via temporal modulation of canonical Wnt signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 29.
  PUBMED    22645348
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 May 29."
COMMENT     Sequence Label: pXL001
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..651
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(104..123)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             652..672
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaggaattga tccttcgaac tagtgccacc atggctagat tagataaaag taaagtgatt
       61 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
      121 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
      181 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
      241 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
      301 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
      361 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
      421 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag
      481 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
      541 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
      601 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc gccaaaaaag
      661 aagagaaagg tcgacggcgg tggtgctttg tctcctcagc actctgctgt cactcaagga
      721 agtatcatca agaacaagga gggcatggat gctaagtcac taactgcctg gtcccggaca
      781 ctggtgacct tcaaggatgt atttgtggac ttcaccaggg aggagtggaa gctgctggac
      841 actgctcagc agatcgtgta cagaaatgtg atgctggaga actataagaa cctggtttcc
      901 ttgggt
//
