LOCUS       sequence-322051-        2880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RDF sequence for C-terminal fusion with PhiC31 recombinase, together
            with a 18-nt linker sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pUPD2 linker-RDF (GB2892)
FEATURES             Location/Qualifiers
     source          1..2880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             752..772
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      817..874
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(898..915)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1069..1657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1149..1168)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1840..1934)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1955..2614)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2529..2548
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2776..2794
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2798..2841)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2844..2865
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ttcgacttct agatctggta ctgctaagag atctatttgg gctggtgatg aagataataa
       61 gcctaagaag agagaaactt atgctgatga tactgttggt agatttcatt ctggttattc
      121 tgaaactaat gaaagaggta aggttgttcc tgttgctctt gataagtgga gaatttctac
      181 tggtgaacaa tctgttgctg atgctgttgc tcaacttttt ggtggtactc ctgttgaaaa
      241 tgaagaatct acttctgaaa attttattga tgtttttact gatagaccta aggttcctgt
      301 tattattgaa gctgatggta ttcattggga tatgaagctt tggcttaatg gtaagcttaa
      361 gcatcattgt gatggttttg attttgtttc tcatgctgat gaagaaatga ttggtcaacc
      421 ttgtggttgt cctaagcttt ttgatgaaag aaaggctgct gctaaggaat atgatgctcc
      481 taatcctgct attactgtta cttttactct tgctgatgat cctgaacttg gtagatttaa
      541 gtttcaaact ggttcttgga ctctttttaa ggttcttcat gaagctgaag atgatgttga
      601 aagagttggt aagggtggtg ctgttcttgc taatcttgaa cttgaacttg ttgaatatac
      661 tcctaagaga ggtcctatga gaaataagct tgtttcttat tataagccta ctattactgt
      721 tcttaagtct tataatgatg ctattgctga tccaaaaaaa aagaggaagg tgtaggcttt
      781 gagaccactc atcgcgacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc
      841 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc
      901 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      961 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1021 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
     1081 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1141 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1201 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1261 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1321 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1381 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1441 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1501 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1561 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1621 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1681 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1741 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1801 tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc
     1861 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1921 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcgtcgc
     1981 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     2041 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     2101 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     2161 tcacccaggg attggctgat acgaaaaaca tattctcaat aaacccttta gggaaatagg
     2221 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     2281 cgtcgtggta ttcactccag agtgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     2341 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     2401 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     2461 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     2521 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     2581 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     2641 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     2701 ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg
     2761 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2821 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagcgatg agtggtctcg
//
