LOCUS       sequence-322054-        2523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2523)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD2 VP16 (GB2717)
FEATURES             Location/Qualifiers
     source          1..2523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             167..400
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             401..421
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      466..523
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(547..564)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(718..1306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(798..817)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1489..1583)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1604..2263)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2178..2197
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2425..2443
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2447..2490)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2493..2514
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gtctcgttcg ggttccgcgt acagccgcgc gcgtacgaaa aacaattacg ggtctaccat
       61 cgagggcctg ctcgatctcc cggacgacga cgcccccgaa gaggcggggc tggcggctcc
      121 gcgcctgtcc tttctccccg cgggacacac gcgcagactg tcgacggccc ccccgaccga
      181 tgtcagcctg ggggacgagc tccacttaga cggcgaggac gtggcgatgg cgcatgccga
      241 cgcgctagac gatttcgatc tggacatgtt gggggacggg gattccccgg gtccgggatt
      301 taccccccac gactccgccc cctacggcgc tctggatatg gccgacttcg agtttgagca
      361 gatgtttacc gatgcccttg gaattgacga gtacggtggg ccaaagaaga agaggaaggt
      421 gtaggctttg agaccactca tcgcgactag tagcggccgc tgcagtccgg caaaaaaggg
      481 caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca
      541 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      601 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      661 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      721 ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      781 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      841 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      901 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      961 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1021 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1081 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1141 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1201 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1261 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1321 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1381 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1441 aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat
     1501 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     1561 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
     1621 actcgtcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
     1681 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     1741 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     1801 tggtgaaact cacccaggga ttggctgata cgaaaaacat attctcaata aaccctttag
     1861 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     1921 gccggaaatc gtcgtggtat tcactccaga gtgatgaaaa cgtttcagtt tgctcatgga
     1981 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     2041 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     2101 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     2161 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     2221 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     2281 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     2341 agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt
     2401 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
     2461 aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagcgatga
     2521 gtg
//
