LOCUS       sequence-322066-        2303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Copper dependent CUP2 binding DNA sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2303)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD2 CBS4 (GB2718)
FEATURES             Location/Qualifiers
     source          1..2303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..116
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(120..163)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    166..187
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      442..499
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(523..540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(694..1282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(774..793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1465..1559)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1580..2239)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2154..2173
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt
       61 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa
      121 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct
      181 tctagagcga tgagtggtct cgggagagtg gtcttgggag agcgatgcgt cttttccgat
      241 gaaccgttcc agcaaaaaag actaagcgat gcgtcttttc cgctgaaccg ttccagcaaa
      301 aaagactaag cgatgcgtct tttccgctga accgttccag caaaaaagac taggagagcg
      361 atgcgtcttt tccgctgaac cgttccagca aaaaagacta tccctgagac cactcatcgc
      421 gactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
      481 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
      541 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      601 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      661 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
      721 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      781 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      841 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      901 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      961 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1021 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1081 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1141 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1201 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1261 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1321 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1381 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1441 ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1501 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1561 aagcgagctc gatatcaaat tacgccccgc cctgccactc gtcgcagtac tgttgtaatt
     1621 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1681 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1741 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1801 ctgatacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1861 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1921 tccagagtga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1981 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     2041 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     2101 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     2161 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     2221 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     2281 atacgcccgg tagtgatctt att
//
