LOCUS       sequence-322071-        2741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UBQ10 promoter from A. thaliana for constitutive expression in plant
            systems..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Bernabe-Orts JM, Casas-Rodrigo I, Minguet EG, Landolfi V, 
            Garcia-Carpintero V, Gianoglio S, Vazquez-Vilar M, Granell A, Orzaez
            D
  TITLE     Assessment of Cas12a-mediated gene editing efficiency in plants.
  JOURNAL   Plant Biotechnol J. 2019 Apr 5. doi: 10.1111/pbi.13113.
  PUBMED    30950179
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2019 Apr 5. doi: 10.1111/pbi.13113."
COMMENT     Sequence Label: pUPD2 AtUBQ10 (GB2478)
FEATURES             Location/Qualifiers
     source          1..2741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     118..136
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(140..183)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    186..207
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      900..957
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(981..998)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1152..1740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1232..1251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1923..2017)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2038..2697)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2612..2631
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
       61 actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
      121 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
      181 ttctggaatt cgcggccgct tctagagcga tgagtggtct cgggagtcga cgagtcagta
      241 ataaacggcg tcaaagtggt tgcagccggc acacacgagt cgtgtttatc aactcaaagc
      301 acaaatactt ttcctcaacc taaaaataag gcaattagcc aaaaacaact ttgcgtgtaa
      361 acaacgctca atacacgtgt cattttatta ttagctattg cttcaccgcc ttagctttct
      421 cgtgacctag tcgtcctcgt cttttcttct tcttctataa aacaataccc aaagagctct
      481 tcttcttcac aattcagatt tcaatttctc aaaatcttaa aaactttctc tcaattctct
      541 ctaccgtgat caaggtaaat ttctgtgttc cttattctct caaaatcttc gattttgttt
      601 tcgttcgatc ccaatttcgt atatgttctt tggtttagat tctgttaatc ttagatcgaa
      661 gacgattttc tgggtttgat cgttagatat catcttaatt ctcgattagg gtttcataga
      721 tatcatccga tttgttcaaa taatttgagt tttgtcgaat aattactctt cgatttgtga
      781 tttctatcta gatctggtgt tagtttctag tttgtgcgat cgaatttgtc gattaatctg
      841 agtttttctg attaacagaa tgtgagacca ctcatcgcga ctagtagcgg ccgctgcagt
      901 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
      961 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1021 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1081 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1141 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1201 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1261 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1321 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1381 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1441 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1501 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1561 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1621 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1681 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1741 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1801 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1861 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
     1921 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     1981 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     2041 cgccccgccc tgccactcgt cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     2101 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     2161 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     2221 gtttaaatca aaactggtga aactcaccca gggattggct gatacgaaaa acatattctc
     2281 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     2341 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagtgatg aaaacgtttc
     2401 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     2461 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     2521 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     2581 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     2641 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     2701 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat a
//
