LOCUS       sequence-322077-        2279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B3 for signal peptide (transit peptide to chloroplast) from Ribulose
            bisphosphate carboxylase small chain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2279)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD2 NtSPrbcS (GB2213)
FEATURES             Location/Qualifiers
     source          1..2279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      215..272
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(296..313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(467..1055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(547..566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1238..1332)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1353..2012)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1927..1946
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2174..2192
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2196..2239)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2242..2263
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 atggcttcct cagttctttc ctctgcagca gttgccaccc gcagcaatgt tgctcaagct
       61 aacatggttg cacctttcac tggccttaag tcagctgcct cattccctgt ttcaaggaag
      121 caaaaccttg acatcacttc cattgccagc aacggcggaa gagtgcaatg cgcagcctga
      181 gaccactcat cgcgactagt agcggccgct gcagtccggc aaaaaagggc aaggtgtcac
      241 caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc
      301 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      361 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      421 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc
      481 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      541 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      601 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      661 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      721 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      781 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      841 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      901 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      961 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1021 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1081 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1141 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1201 tatatgagta aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc
     1261 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     1321 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcgtcgcag
     1381 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     1441 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     1501 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     1561 acccagggat tggctgatac gaaaaacata ttctcaataa accctttagg gaaataggcc
     1621 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     1681 tcgtggtatt cactccagag tgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     1741 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
     1801 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     1861 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     1921 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     1981 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     2041 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     2101 cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     2161 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc
     2221 tttcgctaag gatgatttct ggaattcgcg gccgcttcta gagcgatgag tggtctcga
//
