LOCUS       sequence-322080-        3923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Streptomyces phage PhiC31 integrase, adapted for C-terminal fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pUPD2 PhiC31 integrase (GB1561)
FEATURES             Location/Qualifiers
     source          1..3923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..1816
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     terminator      1860..1917
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1941..1958)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2112..2700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2192..2211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2883..2977)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2998..3657)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3572..3591
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3819..3837
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3841..3884)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3887..3908
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatggacacg tatgccggcg cttatgatag acaatcaaga gaaagagaga actcttctgc
       61 cgcctctcca gctacacaga gatctgccaa cgaggataaa gctgctgatt tacaaagaga
      121 ggtagagaga gatgggggta ggtttagatt tgtgggtcat ttctctgagg ctcctggtac
      181 atcagccttt ggtactgctg aacggcctga atttgaaaga atattaaatg agtgtcgggc
      241 cggtcgacta aatatgataa tagtttatga tgtatctagg ttcagtagac tcaaagttat
      301 ggatgcaatt ccgatcgtga gcgagctact tgcactcggt gttactatcg taagtacaca
      361 agaaggtgta ttcaggcagg gtaatgtgat ggaccttata caccttatta tgcgactcga
      421 cgctagccat aaagagtcat cactgaagag tgctaaaatc ttggacacca agaacctgca
      481 aagggagctt ggaggctatg taggaggaaa agctccgtat ggtttcgaac tcgtgagtga
      541 gacaaaagag attaccagaa atggtaggat ggtgaacgtg gttattaata agctagctca
      601 tagcacaacc cctctcacag ggccttttga gttcgaacct gacgttatta gatggtggtg
      661 gagagagatc aaaactcaca aacatcttcc atttaagcca ggtagccagg ctgctatcca
      721 ccctggatct atcactgggc tttgtaaaag gatggatgct gacgcagtgc caactcgagg
      781 ggaaacaatc ggtaagaaga ctgcatcttc cgcttgggat cccgctacag ttatgagaat
      841 ccttcgggat ccgcgtattg ctggattcgc cgcagaagtc atttataaga aaaaacctga
      901 tggtactcct actacaaaaa tcgaaggtta taggatccaa agggatccta ttactttgcg
      961 tccagttgaa ttagactgtg gaccgatcat cgaacctgcc gagtggtacg agttgcaggc
     1021 ctggcttgat ggtagagggc gtggaaaggg tttatctcgg ggacaggcaa ttttgtcagc
     1081 aatggataag ctttattgtg agtgtggggc tgttatgaca tcaaaacgtg gagaagagtc
     1141 aatcaaggat tcttacaggt gcagacgtcg taaagtggtg gacccttctg ctccaggaca
     1201 gcatgagggt acatgcaacg tttctatggc tgctcttgat aaatttgtgg ccgaacgtat
     1261 tttcaacaag attagacacg ctgaagggga tgaggaaact ttagctcttc tttgggaggc
     1321 tgcccggcgt tttggtaagc taacagaggc accagaaaaa agtggcgaaa gagctaattt
     1381 ggtagccgaa agagcagacg ccttgaatgc attggaagaa ctttacgaag atagggctgc
     1441 cggagcatat gatggaccag ttggtcggaa gcatttcagg aagcaacaag ctgcactaac
     1501 tttgagacag caaggcgcag aggagcgact cgcagagctt gaggccgccg aggccccaaa
     1561 gcttccactc gatcaatggt ttcctgagga tgccgacgct gatcctacag gtccaaagag
     1621 ctggtgggga agagctagcg tagacgataa gagagtgttt gtgggtctat ttgttgacaa
     1681 gattgtagtt actaagtcca ctacgggtcg aggacagggc actcccatcg aaaagagagc
     1741 cagtattacc tgggcaaagc ctccaaccga tgatgatgaa gacgatgcac aagatggtac
     1801 cgaggatgtg gcagcaggtt cgtgagacca ctcatcgcga ctagtagcgg ccgctgcagt
     1861 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
     1921 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1981 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2041 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2101 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2161 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2221 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2281 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2341 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2401 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2461 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2521 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2581 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2641 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2701 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2761 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2821 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
     2881 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     2941 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     3001 cgccccgccc tgccactcgt cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     3061 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     3121 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     3181 gtttaaatca aaactggtga aactcaccca gggattggct gatacgaaaa acatattctc
     3241 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     3301 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagtgatg aaaacgtttc
     3361 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     3421 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     3481 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     3541 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     3601 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     3661 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     3721 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg
     3781 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     3841 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
     3901 ttctagagcg atgagtggtc tcg
//
