LOCUS       sequence-322082-        2355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ethanol inducible operator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2355)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD2 AlcA (GB1549)
FEATURES             Location/Qualifiers
     source          1..2355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      292..349
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(373..390)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(544..1132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(624..643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1315..1409)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1430..2089)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2004..2023
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2251..2269
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2273..2316)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2319..2340
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagcgggat agttccgacc taggattgga tgcatgcgga accgcacgag ggcggggcgg
       61 aaattgacac accactcctc tccacgcacc gttcaagagg tacgcgtata gagccgtata
      121 gagcagagat ggagcacttt ctggtactgt ccgcacggga tgtccgcacg gagagccaca
      181 aacgagcggg gccccgtacg tgctctccta ccccaggatc gcatccccgc atagctgaac
      241 atctatataa tccctgagac cactcatcgc gactagtagc ggccgctgca gtccggcaaa
      301 aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt
      361 tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      421 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      481 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      541 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      601 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      661 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      721 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      781 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      841 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      901 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      961 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1021 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1081 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1141 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1201 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1261 taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc
     1321 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1381 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     1441 cctgccactc gtcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     1501 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     1561 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     1621 caaaactggt gaaactcacc cagggattgg ctgatacgaa aaacatattc tcaataaacc
     1681 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     1741 gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt tcagtttgct
     1801 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     1861 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     1921 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     1981 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     2041 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     2101 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     2161 ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa
     2221 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga
     2281 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag
     2341 cgatgagtgg tctcg
//
