LOCUS       sequence-322085-        2181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hepatitis Delta Virus ribozyme signal for the assembly of gRNA 
            expression cassettes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Bernabe-Orts JM, Casas-Rodrigo I, Minguet EG, Landolfi V, 
            Garcia-Carpintero V, Gianoglio S, Vazquez-Vilar M, Granell A, Orzaez
            D
  TITLE     Assessment of Cas12a-mediated gene editing efficiency in plants.
  JOURNAL   Plant Biotechnol J. 2019 Apr 5. doi: 10.1111/pbi.13113.
  PUBMED    30950179
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2019 Apr 5. doi: 10.1111/pbi.13113."
COMMENT     Sequence Label: pUPD2 HDV (GB1444)
FEATURES             Location/Qualifiers
     source          1..2181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           1..68
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     terminator      118..175
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(199..216)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(370..958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(450..469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1141..1235)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1256..1915)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1830..1849
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2077..2095
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2099..2142)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2145..2166
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggccggcatg gtcccagcct cctcgctggc gccggctggg caacatgctt cggcatggcg
       61 aatgggactt ttttttcgct tgagaccact catcgcgact agtagcggcc gctgcagtcc
      121 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
      181 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      241 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      301 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      361 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      421 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      481 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      541 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      601 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      661 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      721 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      781 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      841 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      901 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      961 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1021 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1081 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg
     1141 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1201 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     1261 ccccgccctg ccactcgtcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     1321 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     1381 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     1441 ttaaatcaaa actggtgaaa ctcacccagg gattggctga tacgaaaaac atattctcaa
     1501 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     1561 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa aacgtttcag
     1621 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     1681 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     1741 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     1801 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     1861 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     1921 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     1981 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
     2041 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     2101 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt
     2161 ctagagcgat gagtggtctc g
//
