LOCUS       sequence-322088-        4293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part 3/3 for assembly of site-specific recombination based 
            Registers. Composed of the 5'UTR sequence of 35S promoter, coding 
            sequence of Luc and NOS terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pUPD2 Assembly Register 3/3 Part - 5'UTR:Luc:T...
FEATURES             Location/Qualifiers
     source          1..4293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     370..389
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(639..682)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    685..706
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             758..2410
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(824..842)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      2437..2689
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      2951..3008
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(3032..3049)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3203..3791)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3283..3302)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3974..4068)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(4089..4293,1..455))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ctcacccagg gattggctga tacgaaaaac atattctcaa taaacccttt agggaaatag
       61 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
      121 tcgtcgtggt attcactcca gagtgatgaa aacgtttcag tttgctcatg gaaaacggtg
      181 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
      241 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
      301 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
      361 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
      421 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
      481 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
      541 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat
      601 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
      661 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagcgat gagtggtctc
      721 gacctaaaca acattacaat ttactattct agtcgaaatg gaagacgcca aaaacataaa
      781 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
      841 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
      901 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
      961 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
     1021 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
     1081 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
     1141 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa
     1201 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
     1261 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag
     1321 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
     1381 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
     1441 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
     1501 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     1561 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
     1621 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     1681 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
     1741 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
     1801 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     1861 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
     1921 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
     1981 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     2041 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
     2101 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
     2161 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     2221 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     2281 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     2341 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
     2401 cgccgtgtaa tgagcttgga atggatcttc gatcccgatc gttcaaacat ttggcaataa
     2461 agtttcttaa gattgaatcc tgttgccggt cttgcgacga ttatcatata atttctgttg
     2521 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     2581 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     2641 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattgcca
     2701 agctaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     2761 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2821 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2881 ataaatgctt caataatggg accgactcgc gcttgagacc actcatcgcg actagtagcg
     2941 gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac
     3001 cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg
     3061 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3121 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3181 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa
     3241 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3301 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3361 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3421 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3481 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3541 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3601 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3661 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3721 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3781 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3841 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3901 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3961 gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     4021 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
     4081 atatcaaatt acgccccgcc ctgccactcg tcgcagtact gttgtaattc attaagcatt
     4141 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     4201 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     4261 atattggcca cgtttaaatc aaaactggtg aaa
//
