LOCUS       sequence-322105-        9508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for negative control of knockdown expressing a 
            non-target miR against Renilla luciferase and expression of a Ly6G 
            surface marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   van der Donk LEH, van der Spek J, van Duivenvoorde T, Ten Brink MS, 
            Geijtenbeek TBH, Kuijl CP, van Heijst JWJ, Ates LS
  TITLE     An optimized retroviral toolbox for overexpression and genetic 
            perturbation of primary lymphocytes.
  JOURNAL   Biol Open. 2022 Feb 15;11(2):bio059032. doi: 10.1242/bio.059032. 
            Epub 2022 Mar 1.
  PUBMED    35229875
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2022-02-15"; volume: "11(2):bio059032. doi"; pages: "
            10.1242/bio.059032. Epub 2022 Mar 1"
COMMENT     Sequence Label: pLenti-EF1a-Ly6G-miR30
FEATURES             Location/Qualifiers
     source          1..9508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1066..1299
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1484..1528
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1826..1943
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1993..3175
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2224..3166
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3123..3143
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3209..3218
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     ncRNA           3655..3776
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3804..3818
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3846..3972
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    4004..4592
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4057..4077)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4475..4486)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4664..4897
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4923..4940)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4929..5153
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5199..5627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5286..5305)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5496..5517
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5636..5656)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5641..5970
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5821..5956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5883..5902
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6018..6065
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6084..6458
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6588..6709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6625..6644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6679..6698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6758..6774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6758..6774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6771..6793)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6782..6798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6806..6812,6813..6830,6831..6836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6851..6872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6989..7006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7160..7748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7240..7259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7919..8710,8711..8779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8542..8561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8780..8884)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8959..8978)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9128..9507
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
      961 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1021 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1081 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1141 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1201 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1261 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1321 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1381 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1441 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1501 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1561 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1621 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     1681 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     1741 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     1801 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     1861 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     1921 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     1981 taattaacgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     2041 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     2101 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2161 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2221 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     2281 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     2341 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     2401 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     2461 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     2521 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     2581 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     2641 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     2701 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     2761 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     2821 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     2881 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     2941 aggcacctcg attagttctc gatcgagctt ttggagtacg tcgtctttag gttgggggga
     3001 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     3061 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     3121 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaaggat
     3181 ccggtcgtgc ggccgcacgg aaacgcgtgc caccatggac acctgtcata tcgccaagag
     3241 ctgcgtgctg atcctgctgg tggttctgct gtgtgccgag cgagcacagg gactggagtg
     3301 ctacaactgt atcggcgtgc cacctgagac aagctgcaac accaccacct gtcctttcag
     3361 cgacggcttc tgtgtggccc tggaaatcga agtgatcgtg gacagccacc gcagcaaagt
     3421 gaagtccaac ctgtgcctgc ctatctgccc caccacactg gacaacaccg agatcacagg
     3481 caacgccgtg aacgtgaaaa cctactgctg caaagaggac ctctgcaacg ccgctgttcc
     3541 aacaggcgga agctcttgga caatggctgg cgtgctgctg ttcagcctgg tgtctgttct
     3601 gctgcagacc ttcctgtgag catgccgcga ccctgcaggt ttctgaagct ttgtttgaat
     3661 gaggcttcag tactttacag aatcgttgcc tgcacatctt ggaaacactt gctgggatta
     3721 cttcttcagg ttaacccaac agaaggctcg agaaggtata ttgctgttga cagtgagcga
     3781 aagacaatga gtacacagca atagtgaagc cacagatgta ttgctgtgta ctcattgtct
     3841 tctgcctact gcctcggaat tcaaggggct actttaggag caattatctt gtttactaaa
     3901 actgaatacc ttgctatctc tttgatacat ttttacaaag ctgaattaaa atggtataaa
     3961 ttaaatcact ttatcgatac ggaaacgcgc gcgttaagtc gacaatcaac ctctggatta
     4021 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     4081 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4141 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4201 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4261 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4321 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4381 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     4441 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4501 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     4561 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     4621 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     4681 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     4741 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     4801 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     4861 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     4921 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4981 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5041 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5101 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     5161 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     5221 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5281 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5341 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5401 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5461 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5521 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5581 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     5641 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5701 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5761 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5821 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5881 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     5941 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     6001 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     6061 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     6121 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     6181 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     6241 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     6301 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     6361 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     6421 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     6481 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6541 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     6601 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6661 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     6721 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6781 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6841 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6901 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6961 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7021 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7081 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7141 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7201 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7261 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7321 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7381 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7441 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7501 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7561 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7621 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7681 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7741 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7801 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7861 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7921 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7981 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8041 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8101 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8161 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8221 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8281 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8341 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8401 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8461 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8521 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8581 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8641 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8701 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8761 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8821 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8881 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     8941 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     9001 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     9061 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     9121 tcgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     9181 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     9241 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     9301 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     9361 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     9421 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     9481 tacgtattag tcatcgctat taccatgg
//
