LOCUS       sequence-322130-        7218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dominant-negative construct to inhibit Notch signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Chen X, Emerson MM
  TITLE     Notch signaling represses cone photoreceptor formation through the 
            regulation of retinal progenitor cell states.
  JOURNAL   Sci Rep. 2021 Jul 15;11(1):14525. doi: 10.1038/s41598-021-93692-w.
  PUBMED    34267251
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-07-15"; volume: "11(1):14525. doi"; pages: " 
            10.1038/s41598-021-93692-w"
COMMENT     Sequence Label: CAG-RbpjEnR
FEATURES             Location/Qualifiers
     source          1..7218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4114..4143
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4236..4255)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4301..4356
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4355..4374)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4717..4733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4717..4733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4730..4752)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4741..4757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4765..4771,4772..4789,4790..4795))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4810..4831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4890..5085
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4936..5071
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5091..5225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5128..5147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5182..5201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5293..5310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5464..6052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5544..5563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6223..7014,7015..7083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6846..6865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7084..7188)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gcgcgcgccr ggsggggggg gggggggggg gggggggggg
      541 ggggcgrggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggrggg ctcgggggrg gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcac catggcgcct
     1741 gttgtgacag ggaagtttgg tgagcggcct ccacccaaac gactcactag ggaagctatg
     1801 cgaaattatt taaaagaacg aggggatcaa acagtgctca ttcttcatgc aaaagttgca
     1861 cagaagtctt acggaaatga aaaacgattt ttttgccctc ctccttgtgt gtatcttatg
     1921 ggcagtggtt ggaagaaaaa aaaagagcaa atggaacgag atggatgttc tgaacaagag
     1981 tctcaaccct gtgcgtttat tggaatagga aacagtgacc aagaaatgca gcagctgaac
     2041 ttggaaggga agaactactg cacagccaaa acattgtaca tatctgattc agacaagaga
     2101 aaacatttca tgttgtctgt aaagatgttc tatggcaaca gcgatgacat tggtgtgttc
     2161 ctcagcaagc ggataaaggt catctccaaa ccctccaaaa agaagcagtc actgaagaat
     2221 gctgacttgt gcattgcttc aggaacgaag gtggcactgt tcaatcgcct tcggtcccag
     2281 acagttagta ccaggtacct gcatgtagaa ggagggaatt tccacgccag ttcacaacag
     2341 tggggagcat tttacatcca tctcttggac gacgacgagt cggaaggaga ggagttcaca
     2401 gttagagatg gctacatcca ttacgggcag actgtcaagc ttgtgtgctc agtgactggc
     2461 atggcactcc caagattgat aattaggaaa gttgataagc agacggcatt actggatgca
     2521 gacgaccctg tatcacaact ccacaaatgt gcattttacc ttaaggatac agaaagaatg
     2581 tacttgtgcc tttctcaaga aagaataatc caatttcagg ccactccatg tccaaaagaa
     2641 caaaataagg aaatgataaa cgatggagct tcctggacaa tcattagcac agacaaggca
     2701 gaatacacgt tctatgaggg aatgggcccc gtccttgccc cagtcacccc tgtgcctgtc
     2761 gtagaaagtc ttcagttgaa tggcggcggg gacgtagcaa tgcttgaact tacaggacaa
     2821 aactttactc caaatttacg agtgtggttt ggggatgtag aagccgaaac aatgtacaga
     2881 tgtggagaga gcatgctctg tgtggtccca gacatttctg cattccggga aggttggaga
     2941 tgggtccgcc agccagtcca ggttccagtg actttggtcc gaaatgacgg ggtcatttac
     3001 tccaccagcc ttaccttcac ctacacacca gagccagggc cgaggccaca ctgcagcgct
     3061 gcaggagcga ttctcagagc caactccagc caagtgccct ccaatgagtc aaacacaaac
     3121 agcgagggga attacacaaa tgccagcaca aattctacca gtgtcacatc gtccacagca
     3181 accgtggtgt ccgtggaatt tcgcgatgcc ctggaggatc gctgcagccc acagtcagcg
     3241 cccagcccca ttaccctaca aatgcagcat cttcaccacc agcaacagca gcagcagcaa
     3301 cagcagcagc aaatgcagca cctccaccag ctgcagcaac tgcagcagtt gcaccaacag
     3361 caactggccg ccggtgtctt ccaccatccg gcaatggcct tcgatgccgc tgcagccgcc
     3421 gctgctgcag ctgctgctgc ggccgcccac gctcatgctg ctgcactgca gcagcgcctc
     3481 agtggcagtg gatcgcccgc atcctgctcc acgcccgcct cgtccacgcc gctgaccatc
     3541 aaggaggagg aaagcgactc cgtgatcggt gacatgagtt tccacaatca gacgcacacc
     3601 accaacgagg aggaggaggc ggaggaggat gacgacattg atgtggatgt ggatgatacg
     3661 tcggcgggcg gacgcctgcc accacccgcc caccagcagc agtcgacggc caagccctcg
     3721 ctggcctttt ccatctccaa catcctgagc gatcgtttcg gagatgtcca gaagcctggc
     3781 aagtcgattg agaaccaggc cagcatattc cgccccttcg aggcgaatcg ctcccagact
     3841 gccacgccct ccgcctttac aagagtggat ctgctggagt ttagccggca acagcaagct
     3901 gccgccgcag ccgctactgc ggccatgatg ctggaacggg ccaacttcct taactgcttc
     3961 aatccggctg cctatcccag gatacacgag gaaatcgtgc agagtcgcct gcgcaggagt
     4021 gcagccaatg ccgtcatccc gccgcccatg agctccaaga tgagcgatgc caatccagag
     4081 aaatctgctc tgggatctat gcagcccaag cttgagcaga agctgatctc cgaggaggac
     4141 ctgtaagaat tcggccgcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     4201 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     4261 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     4321 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     4381 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     4441 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     4501 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     4561 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     4621 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     4681 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4741 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4801 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4861 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     4921 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4981 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     5041 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
     5101 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5161 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5221 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5281 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5341 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5401 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5461 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5521 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5581 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5641 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5701 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5761 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5821 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5881 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5941 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6001 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6061 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6121 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6181 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6241 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6301 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6361 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6421 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6481 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6541 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6601 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6661 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6721 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6781 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6841 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6901 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6961 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7021 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7081 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7141 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7201 aaaagtgcca cctgggtc
//
